Validation of a Small Animal Model for Soft Tissue Filler Characterization

Abstract

The rat subcutis model demonstrated the ability to differentiate between HA fillers with different residence times. The caliper-based rat-subcutis method demonstrated consistent volumetric analysis and correlated with human residence times of HA fillers. These quantitative results validate the rat subcutis model as an expedited preclinical model for HA fillers.

Effects of crosslinked dextran in hydroxylpropyl methylcellulose on soft tissue augmentation in rats

“…DiHMs (pH 5 and 7) were evaluated to determine their efficacy and safety in rats before clinical evaluation and commercialization. Previous experiments indicated that intradermal injections of soft tissue fillers in a rat model result in epidermal injury . Similar to the current study, subcutaneous injection of soft tissue fillers in a rat model to evaluate local effects after implantation is frequently used because of better efficiency and affordability …”

“…Previous experiments indicated that intradermal injections of soft tissue fillers in a rat model result in epidermal injury. 24 Similar to the current study, subcutaneous injection of soft tissue fillers in a rat model to evaluate local effects after implantation is frequently used because of better efficiency and affordability. 24 The crosslinked dextran used in this study was similar to previous dextranomer-based implants using a rat model with the exception of an HPMC suspension.…”

“…24 Similar to the current study, subcutaneous injection of soft tissue fillers in a rat model to evaluate local effects after implantation is frequently used because of better efficiency and affordability. 24 The crosslinked dextran used in this study was similar to previous dextranomer-based implants using a rat model with the exception of an HPMC suspension. 14,25 In addition, sequential changes in gross surface appearance, sizes, collagen formation, and histological analysis at regular intervals up to 24-month PI were well-established in this study.…”

Comparison of 3 Techniques of Fat Grafting and Cell-Supplemented Lipotransfer in Athymic Rats

“…Volume retention of the grafts measured with a digital caliper allowed for continuous monitoring of graft volume over the course of the study rather than obtaining a single end-point value. 22 This approach allowed for the measurement of external factors such as skin thickness, which can increase as animals age. This is likely the reason why overall volume retention was high.…”

Long-lasting and bioactive hyaluronic acid-hydroxyapatite composite hydrogels for injectable dermal fillers: Physical properties and in vivo durability

“…For the evaluation of volumetric maintenance, MRI techniques are an efficient method for measuring filler volume. 43,44 All of the fillers were easily detected and the three-dimensional structures were visualized by MRI owing to their high levels of hydration. 45 Pure HAc showed the brightest images because its main component is water.…”