Validation of an electrospray ionization LC/MS/MS method for quantitative analysis of vincristine in human plasma samples

An HPLC method for the pharmacokinetic study of vincristine sulfate-loaded PLGA–PEG nanoparticle formulations after injection to rats

“…There have been several methods described previously to analyze vincristine in plasma after the administration of VCR injection, from HPLC-UV [1,2,[7][8][9], HPLC combined with electrochemical detection method [10], to LC-MS [11,12], then to LC-MS/MS [13][14][15][16][17][18][19][20]. However, these assay methods are comparatively complex; generally the mobile phases consist of several components and the pH values are required to be adjusted.…”

An LC‐MS/MS method for simultaneous determination of vincristine and verapamil in rat plasma after oral administration of a dual agent formulation

“…For the determination of vincristine in plasma, radioimmunoassay (Nelson, 1982), HPLC (Koopmans et al, 2001;Embree et al, 1997;Bloemhof et al, 1991) and liquid chromatographicmass spectrometric methods (Dennison et al, 2008;Lee et al, 2007;Skolnik et al, 2006;Corona et al, 2008;Guilhaumou et al, 2010) have been reported. Some of these methods used a large volume of plasma sample (0.3-4 mL) or needed longer chromatographic run time (8 min), while others employed complicated extraction processes (on-line extraction step after precipitation).…”

Mass spectrometry in the pharmacokinetic studies of anticancer natural products

“…MS introduction increases specificity (Ramírez et al, ), clarifies metabolites structure (Puozzo et al, ; de Graeve et al, ), and makes possible the simultaneous quantification of metabolites (Zorza et al, ; Dennison et al, ). Most of published LC‐MS/MS methods are based on positive Electrospray ionization of vincristine (Skolnik et al, ; Lee et al, ; Dennison et al, ; Damen et al, ; Guilhaumou et al, ), while only two reported the use of APCI method (Schmidt et al, ; Corona et al, ). The presence of basic amines in its structure ensures good ionization efficiency, with quantitation limits down to 0.05 ng/mL samples when operating in high temperature ESI conditions (Lee et al, ; Dennison et al, ).…”

“…Early LC-MS methods developed for vincristine required 2/1.5 mL of plasma samples (Ramrez et al, 1997), while recently published methods lowered plasma samples volumes down to 0.1 mL (Corona et al, 2008;Damen et al, 2009c). The proposed extraction procedures were based on traditional offline SPE (Skolnik et al, 2006;Lee et al, 2007b;Guilhaumou et al, 2010) or LLE (Dennison et al, 2008); the best performance in term of recovery % (90-95%) were ensured by a PP followed by an on-line SPE purification (Corona et al, 2008). In all the above mentioned methods, the internal standard chosen was the semi-synthetic drug vinorelbine and chromatographic separation were performed in presence of ammonium acetate both in acidic or basic conditions.…”

“…PK in paediatric patients has been extensively evaluated in the recent years (Groninger et al, 2002;Schmidt et al, 2006;Damen et al, 2009c;Guilhaumou et al, 2010) due to the large interest of vinca alkaloids in the paediatric leukemias treatment. In some papers, the very low amount of plasma (30 mL) necessary to vincristine analysis was due to an increased ionization efficiency obtained with ESI sources (Damen et al, 2009c;Guilhaumou et al, 2010). In other papers, the minimal sample size necessary to perform the analysis by LC-MS was around 200 mL of plasma (Guilhaumou et al, 2010).…”