Validation of radioimmunoassay for estradiol-17/3 by isotope dilution-mass spectrometry and by a test of radiochemical purity

Xing, Shu; Cekan, S.Z.; Diczfalusy, Ulf; Falk, Olle; Gustafsson, Sven A.; Åkerlöf, Eva; Björkhem, Ingemar

doi:10.1016/0009-8981(83)90135-3

Cited by 32 publications

(12 citation statements)

References 26 publications

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“…The changing degree of overestimation by the rapid method in the present study is in accordance with our earlier observations that the specificity of a rapid assay decreases with decreasing plasma levels (13,14). The common factor causing overestimations in the assays of plasma and urine samples seems, therefore, to be a relatively large concentration of crossreacting factors when the concentration of progesterone is low.…”

Section: Discussionsupporting

confidence: 92%

Reliability of Progesterone Measurements in Urine

Beksaç¹,

Cekan²

1985

Clinical Chemistry and Laboratory Medicine

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A radioimmunoassay (RIA) of progesterone in urine is described. After the addition of labelled progesterone, morning urine was extracted with -hexane and the residue was either directly subjected to RIA, or chromatographed on celite prior to RIA. The progesterone from celite chromatography was radiochemically pure. RIA after chromatography was therefore considered valid. The non-chromatographed procedure resulted in overestimations, the degree of which was inversely proportional to progesterone content. The results obtained by the two procedures were well correlated (r = % 0.88 and 0.93, for 2 different groups of samples). Zuverlässigkeit von Progesteronbestimmungen im HarnZusammenfassung: Ein Radioimmunassay zur Bestimmung von Progesteron im Harn wird beschrieben. Morgenharn wurde mit -Hexan extrahiert und der Rückstand entweder direkt oder nach Chromatographie an Celit dem Radioimmunassay unterzogen. Ein im Wanderungsbereich von Progesteron durchgeführter Test auf radiochemische Reinheit zeigte, daß radiochemisch reines Progesteron gemessen wurde. Der Radioimmunassay nach Chromatographie wurde deshalb als gültig angesehen. Ohne vorangehende Chromatographie wurden zu hohe Werte erhalten, deren Abweichung vom Zielwert umgekehrt proportional zum Progesterongehalt war. Beide Verfahren korrelierten gut miteinander (r = 0,88 und 0,93 für zwei Gruppen von Proben).or chemijuminescence assay of a progesterone metabolite -pregnanediol glucuronide (2, 3). These assays require, however, special reagents (a labelled glucuronide and an antiserum to the glucuronide) which are not generally available. In addition, the purification of the glucuronides, both labelled and non-labelled, is laborious and difficült. From this point of view, a RIA progesterone in urine would be much more covenient.RIA of progesterone in urine has been described earlier (4-7). In nöne of the publications, however, was a valid procedure presented, i.e. none of the procedures was shown to produce accurate results. The aim of the present investigation was to develop, for progesterone in urine, a RIA procedure validated by the test of radiochemical purity (8).

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Section: Discussionsupporting

confidence: 92%

Reliability of Progesterone Measurements in Urine

Beksaç¹,

Cekan²

1985

Clinical Chemistry and Laboratory Medicine

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“…Estradiol 17-_ (E2) was determined by the radio-immune assay method according to Xing et al (1983) using kit Immunchem provided by Diagnostic products corporation, Los Angeles. Total testosterone (T) was determined by radio immuno assay method according to Abraham (1977) using kit (Coat-A-count) provided by Orion Diagnostic Spectria , Fin land.…”

Section: Methodsmentioning

confidence: 99%

Toxicological Effect of Butataf Herbicide on Some Physiological Aspects and the Reproductive Performance of Nile Tblapia Oreochromis Niloticus

Shalaby

Mousa²,

Tag³

2007

Egypt. J. of Aquatic Biolo. and Fish.

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Adel.M. Shaiabyefa/. high levels in fish groups exposed to the high concentrations of the herbicide, also, glucose and total plasma lipids were increased significantly in all treated groups. Meanwhile the total plasma protein was strongly decreased and the effects were dose-dependent, as well as the fecundity, gonado-somatic index and hepato-somatic index. Estradiol and testosterone hormones showed severe changes in females and males specially those exposed to the high concentrations of butataf herbicide. Also, T3 and T4 recorded the same trend.

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“…Determination of testicular 17beta-estradiol was performed according to the method of Xing et al (1983) using kits purchased from "IMMUNOTECH".…”

Section: Hormonal Assaymentioning

confidence: 99%

Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes of Rabbit Bucks

Saleh¹,

Sawiress²,

Tony³

et al. 2015

JAS

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As optimization of farm animals reproductive performance is a main objective, the present study was, undertaken to investigate the possible protective effect of vitamin C, vitamin E and olive pomace against dizocilpine (MK-801) induced oxidative stress and its resultant alterations on antioxidant status, spermiogram, hormonal, enzyme markers and histomorphology of testes of rabbit bucks during first and second month of the experiment. For this purpose thirty six male New Zealand White rabbits bucks were equally and randomly divided into six groups (6 in each) namely control injected with 1.0 ml sterile saline; second group was injected by dizocilpine at a dose 0.1 mg/kg. intraperitoneally (i.p.) for 5 consecutive days, the third group was subjected to dizocilpine and supplemented with "vitamin C" (1 g/L of drinking water) 1 g contain 100 I.U. vit C, while the forth group was subjected to dizocilpine and supplemented with "vitamin E" (50 ml/100 L of drinking water,1ml contain 20 I.U. vit E,) and those of the fifth group were supplemented with "vitamin C" plus "vitamin E". All five groups were fed the same basal diet, while the sixth group was subjected to dizocilpine and fed diet which contains olive cake meal (10%). Results in first month revealed (1) significant decrease in spermiogram, antioxidative parameters, testicular estradiol, testosterone, enzyme markers and clear pathological changes in testes of dizocilpine group; (2) Significant improvement in the measured parameters of groups subjected to dizocilpine and supplemented with "vitamin E", "vitamin C" and olive pomace; (3) significant increase of all measured parameters in the "vitamin C" plus "vitamin E" supplemented group. On the other hand, results at second month showed no difference between all groups in these parameters. Conclusively, it was obvious that the supplementation with vitamin C or/and vitamin E and olive pomace to male rabbits exposed to oxidative stress was associated with improved spermiogram, anti-oxidative parameters, hormonal and testicular enzymatic activities.

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Validation of radioimmunoassay for estradiol-17/3 by isotope dilution-mass spectrometry and by a test of radiochemical purity

Cited by 32 publications

References 26 publications

Reliability of Progesterone Measurements in Urine

Reliability of Progesterone Measurements in Urine

Toxicological Effect of Butataf Herbicide on Some Physiological Aspects and the Reproductive Performance of Nile Tblapia Oreochromis Niloticus

Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes of Rabbit Bucks

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