Validation of the AMPFlSTR® SGM Plus™ system for use in forensic casework

Cotton, E.A; Allsop, R.F; Guest, Jennifer; Frazier, R.; Koumi, P.; Callow, I.P; Seager, Anthea; Sparkes, Rebecca

doi:10.1016/s0379-0738(00)00182-1

Cited by 169 publications

(88 citation statements)

References 10 publications

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“…However, we presume that they were due to degradation in this study, because complete loss of amplification in the PCR of the present five systems (Table 2) was found in only four DNA samples extracted under the similar conditions. Imbalance of STR allele in the amplification using degraded DNA is also reported by others 5,26) . Allele drop in the amplification of STRs from highly degraded DNA has been reported by many researchers 2,5,18,22,23,25) .…”

Section: Discussionsupporting

confidence: 71%

“…Imbalance of STR allele in the amplification using degraded DNA is also reported by others 5,26) . Allele drop in the amplification of STRs from highly degraded DNA has been reported by many researchers 2,5,18,22,23,25) . It can be speculated that, when allelic imbalance was observed, the size of amplifiable template DNA might be close to the PCR product size.…”

Section: Discussionsupporting

confidence: 71%

“…Because analysis of short tandem repeat (STR) polymorphisms has high discrimination power and the products databases 4,5,24,25) . As for single nucleotide polymorphisms (SNPs), the HLA-DQ␣ Amplitype typing system 3,26) and the AmpliType PM PCR amplification and typing kit are both commercially available and have been validated.…”

Section: Introductionmentioning

confidence: 99%

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INFLUENCE OF TEMPLATE DNA DEGRADATION ON THE GENOTYPING OF SNPs AND STR POLYMORPHISMS FROM FORENSIC MATERIALS BY PCR

Utsuno

Minaguchi

2004

Bull. Tokyo Dent. Coll.

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Detection of single nucleotide polymorphisms (SNPs) and short tandem repeat (STR) polymorphisms by PCR is widely used to analyze degraded DNAs in forensic science. The success of DNA analysis from human remains largely depends on the quality of the template DNA. We examined two SNPs (HLA-DQA1 and ABO) and two STR polymorphisms (VWA and CD4) by SSCP gel or denaturing gel electrophoresis, using two kinds of degraded DNA samples (165 teeth and blood stains contaminated with saliva) derived from the same person and investigated the influence of template DNA degradation on genotyping. As the degradation of DNA proceeds, unbalanced amplification of alleles occurred in the analysis of both SNPs and STRs, followed by allele drop, and further by loss of amplification. Non-target allelic products of STRs were amplified from highly degraded DNA samples; however, false allelic products of SNPs were not amplified from them. Amplification efficiency increased in proportion to the decrease of PCR target size, but reduction of the PCR target sizes also increased the chances of amplifying contaminating DNA, especially in highly degraded DNA specimens. The present results will help investigators to evaluate the genotyping of highly degraded DNA samples in forensic casework.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 71%

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INFLUENCE OF TEMPLATE DNA DEGRADATION ON THE GENOTYPING OF SNPs AND STR POLYMORPHISMS FROM FORENSIC MATERIALS BY PCR

Utsuno

Minaguchi

2004

Bull. Tokyo Dent. Coll.

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“…However, as this method requires a large amount of material and has low quality results, several cases could not be solved, especially when only little biological material samples were collected in a crime scene investigation. The introduction of the polymerase chain reaction (PCR) technique, which makes possible the amplification of small DNA samples, widened the scopes in Forensic Genetics [8]. In addition, newer DNA tools, including mitochondrial DNA and SNP (single nucleotide polymorphism replacements, insertions or deletions that occur at single positions in the human genome), might be used when STR typing fails to yield a result or when only a partial profile is obtained due to the size and conditions of the sample [9].…”

Section: Literature Review Backgroundmentioning

confidence: 99%

“…However, this documentation may be unavailable or incomplete. At present, with the application of biomolecular resources for human identification, it is possible to identify a person using small amounts of deteriorated biological material, conditions that are relatively frequent in forensic analysis [8]. This fact could be demonstrated after the South Asian tsunami disaster on December 26th 2004, when the most varied techniques were applied for identification of thousands of victims, such as forensic pathology, forensic dentistry, DNA profiling and fingerprinting.…”

Section: Dna and Forensic Dentistrymentioning

confidence: 99%

Use of DNA Technology in Forensic Dentistry

Shambulingappa¹,

Soheyl²,

Gupta³

et al. 2012

J Forensic Res

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The discovery of DNA plays vital role in Human identification is one of the major fields of study and research in forensic science. This discovery was the basis for the development of techniques that allow characterizing each person's individuality based on the DNA sequence. Individual identification of ancient human remains the most fundamental requisites for studies of paleo-population genetics, including kinship among ancient people, intra-and inter population structures in ancient times, and the origin of human populations. However, knowledge of these subjects has been based mainly on circumstantial archaeological evidence for kinship and intra population structure and on genetic studies of modern human populations. Tooth and several biological materials such as bone tissue, hair bulb, saliva, blood and other body tissues may be employed for isolation of DNA and accomplishment of laboratory tests for human identification. Here we describe individual identification of ancient humans by using short-nucleotide tandem repeats and mitochondrial DNA (mtDNA) extracted from a tooth as genetic markers. The application of this approach to kinship analysis shows clearly the presence or absence of kinship among the ancient remains examined.

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Short tandem repeat polymorphism linkage to the androgen receptor gene in prostate carcinoma

Riley

Krieger

2001

Cancer

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BACKGROUND Due to high polymorphism, common sequences, and ubiquitous presence, short tandem repeats (STRs) may enhance genomic typing to determine prostate carcinoma (CaP) predisposition. The human phosphoglycerate kinase (PGK1) gene is located within Xq11‐Xq13, a region implicated in familial prostate carcinoma, androgen insensitivity, perineal hypospadias, and other genitourinary abnormalities. The PGK1 STR is the most polymorphic site described in the Xq11‐Xq13 interval and was investigated for its ability to detect differences comparing a heterogeneous CaP population versus controls. METHODS We compared PGK1 STR allele sizes in 103 localized CaP patients with 299 control subjects to evaluate the STR's ability to detect potential CaP predisposing genetic factors. Allele sizes were measured with an automated DNA sequencer after polymerase chain reaction (PCR) based copying of the PGK1 STR region. Allele sizes were compared using chi square and Mann‐Whitney U tests. RESULTS Among 402 subjects, there were 10 distinct allele sizes consisting of five common and five relatively rare alleles. The PGK1 STR, 12 allele (12 tetrameric repeats) was more common among patients with CaP (p=0.03). Allele 13 was more common in CaP patients > 60 years old than among younger patients (p< 0.005). CONCLUSIONS Our findings suggest that STRs in the Xq11‐Xq13 region and other regions may provide a means to rapidly scan genetic loci in large populations of CaP patients and controls. Within limitations, STRs offer the advantage of relatively uniform protocols that could potentially provide a means to comprehensively scan genomes at known predisposing loci. Cancer 2001;92:2603–8. © 2001 American Cancer Society.

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Validation of the AMPFlSTR® SGM Plus™ system for use in forensic casework

Cited by 169 publications

References 10 publications

INFLUENCE OF TEMPLATE DNA DEGRADATION ON THE GENOTYPING OF SNPs AND STR POLYMORPHISMS FROM FORENSIC MATERIALS BY PCR

INFLUENCE OF TEMPLATE DNA DEGRADATION ON THE GENOTYPING OF SNPs AND STR POLYMORPHISMS FROM FORENSIC MATERIALS BY PCR

Use of DNA Technology in Forensic Dentistry

Short tandem repeat polymorphism linkage to the androgen receptor gene in prostate carcinoma

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