Introduction: The objective was to validate a virtual reality simulation ultrasound model as a tool for training in the use of transvaginal ultrasonography in gynaecology and early pregnancy. Methods: Three separate groups consisting of novice and intermediate level residents as well as expert ultrasound examiners were recruited to the study. All were asked to answer a questionnaire regarding demographic data and ultrasound experience. They subsequently completed two modules: basic gynaecology and early pregnancy, followed by corresponding assessments using a high-fidelity simulator (Scantrainer; Medaphor TM , Cardiff, UK). Finally, the expert group completed an additional questionnaire about various elements of the simulator using a 5-point Likert scale. Results: Each group consisted of eight participants. Overall, the participants agreed that simulation played a role in training (Novices: 75% (n = 6); Intermediates: 100% (n = 8); Experts: 75% (n = 6)). For the degree of realism of the target objects in the gynaecology and early pregnancy module environments compared to a real-patient environment, the simulator was rated satisfactory or very satisfactory by 88% (n = 7) and 75% (n = 6) of experts, respectively. All experts rated the overall usefulness of the content of the simulator for learning fundamental ultrasound technical skills compared to current training methods to be at least satisfactory. When reviewing the assessment scores, experts scored higher than non-experts in gynaecology (P = 0.002) and early pregnancy modules (P = 0.03). Discussion: Face, content and construct validity were demonstrated by the virtual reality ultrasound simulator, suggesting it may be an effective method for training ultrasound skills in gynaecology and early pregnancy to non-expert residents.