Vanillin inhibits PqsR-mediated virulence in <i>Pseudomonas aeruginosa</i>

Mok, Nicholas; Chan, Shepherd Yuen; Liu, Sylvia Yang; Chua, Song Lin

doi:10.1039/d0fo00046a

Cited by 47 publications

(41 citation statements)

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“…Thus, Paeonol could inhibit the major lactones (C4-HSL, 3-oxo-C12-HSLs, and PQS) that regulated the expression of virulence factors produced by P. aeruginosa . These data corroborated with the previous literature where, virulence phenotypes, QS-related genes and biofilm formation of P. aeruginosa was descreaced to varying levels by plant-derived compounds and phenolic compounds ( Rudrappa and Bais, 2008 ; Vandeputte et al, 2010 ; Mok et al, 2020 ; Rather et al, 2021 ). The reason may be attributed to the fact that P. aeruginosa has a complex QS regulating network, including three main QS circuits, and las , the master regulator that induces the expression of both rhl and pqs ( Deziel et al, 2004 ).…”

Section: Discussionsupporting

confidence: 92%

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

et al. 2021

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With the prevalence of multidrug-resistant bacteria and clinical -acquired pathogenic infections, the development of quorum-sensing (QS) interfering agents is one of the most potential strategies to combat bacterial infections and antibiotic resistance. Chinese herbal medicines constitute a valuable bank of resources for the identification of QS inhibitors. Accordingly, in this research, some compounds were tested for QS inhibition using indicator strains. Paeonol is a phenolic compound, which can effectively reduce the production of violacein without affecting its growth in Chromobacterium violaceum ATCC 12472, indicating its excellent anti-QS activity. This study assessed the anti-biofilm activity of paeonol against Gram-negative pathogens and investigated the effect of paeonol on QS-regulated virulence factors in Pseudomonas aeruginosa. A Caenorhabditis elegans infection model was used to explore the anti-infection ability of paeonol in vivo. Paeonol exhibited an effective anti-biofilm activity against Gram-negative bacteria. The ability of paeonol to interfere with the AHL-mediated quorum sensing systems of P. aeruginosa was determined, found that it could attenuate biofilm formation, and synthesis of pyocyanin, protease, elastase, motility, and AHL signaling molecule in a concentration- and time-dependent manner. Moreover, paeonol could significantly downregulate the transcription level of the QS-related genes of P. aeruginosa including lasI/R, rhlI/R, pqs/mvfR, as well as mediated its virulence factors, lasA, lasB, rhlA, rhlC, phzA, phzM, phzH, and phzS. In vivo studies revealed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans, showing a moderate protective effect on C. elegans. Collectively, these findings suggest that paeonol attenuates bacterial virulence and infection of P. aeruginosa and that further research elucidating the anti-QS mechanism of this compound in vivo is warranted.

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Section: Discussionsupporting

confidence: 92%

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

et al. 2021

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“…Reports on vanillin suggest that it can inhibit short-chain homoserine lactones and long-chain acyl-homoserine lactones in Aeromonas hydrophila (Ponnusamy et al 2009). Recently, the in vitro analysis in P. aeruginosa and in silico docking studies revealed that vanillin binds to the active site of PqsR (PQS-binding response regulator) and inhibits pqs expression which is associated with pyocyanin (quorum sensing molecule) and the virulence thereafter (Mok et al 2020).…”

Section: Anti-quorum Sensing Activitymentioning

confidence: 99%

Vanillin: a review on the therapeutic prospects of a popular flavouring molecule

Arya

Rookes

Cahill

et al. 2021

ADV TRADIT MED (ADTM)

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“…A furanone from the algae Delisea pulchra, which is a competitor of QS signal and prevents the activation of regulatory proteins in some bacteria, was the first compound reported to inhibit QS (Hentzer et al 2002). Anti-QS compounds were also detected in plants and studied for many applications (Quecan et al 2019;Mok et al 2020;Kumar & Arthanareeswaran 2019). Several natural compounds that interact with the QS system have also been identified using the in silico approach (Ahmad et al 2017;Satari et al 2021).…”

Section: Resultsmentioning

confidence: 99%

Screening of some Indonesian medicinal plant extracts for anti quorum sensing activity to prevent Aeromonas hydrophila infection in Oreochromis niloticus

et al. 2021

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Abstract. Pangastuti A, Sari SLA, Budiharjo A, Fitri ST, Sayekti P, Putri SR. 2021. Screening of some Indonesian medicinal plant extracts for anti quorum sensing activity to prevent Aeromonas hydrophila infection in Oreochromis niloticus. Biodiversitas 22: 3517-3522. Aeromonas hydrophila disease is a threat to Indonesia's aquaculture and fishing industries because of its results in mass deaths. Efforts to treat and prevent A. hydrophila infection in fish have so far been limited to the use of antibiotics. The use of these antibiotics can result in bacterial resistance as well as issues related to fish quality and environment. Another option is to target the bacterial quorum sensing (QS) system, a bacterial intercellular communication system that uses signal molecules, regulates the expression of A. hydrophila virulence factors such as exoprotease enzymes, elastase, and biofilms. The expression of A. hydrophila virulence factor can be inhibited by using QS inhibitor compounds, preventing infection from starting. The purpose of this study was to screen some Indonesian medicinal plant extracts for anti-QS activity and then use the selected extract to prevent A. hydrophila infection in Tilapia fish (Oreochromis niloticus). With the administration of extract, QS inhibition was observed in vitro based on the production of violacein in Chromobacterium violaceum; enzyme exoprotease, elastase, and the formation biofilm in A. hydrophila. Tilapia fishes were fed with extract supplementation and then infected with A. hydrophila in an in vivo infection challenge test. These test fishes were divided into six treatment groups given a different concentration of extract in feed (Healthy fish with a concentration of 0 g/100 g of feed as control; infected fish with a concentration of 0 g/100 g of feed; infected fish with a concentration of 0.2 g/100 g of feed; infected fish with a concentration 0.4 g/100 g of feed; infected fish with a concentration of 0.6 g/100 g of feed; infected fish with a concentration of 0.8 g/100 g of feed). Fish were reared for 2 weeks with the frequency of feeding 3 times a day. The variables observed included fish survival rate, behavior (swimming, agility, movement, and frequency of operculum opening), and morphology of the test fish. Data analysis of virulence factors production, fish survival rate, fish appetite, and frequency of operculum opening was carried out using the ANOVA variant test, continued with a 5% DMRT test. Others were analyzed descriptively. Of all the extracts studied, 4 extracts showed inhibition of the production of the violaceum pigment in C. violaceum. Muntingia calabura leaves extract was chosen for further testing due to its ease of availability and safety. The extract was significantly decreased the production of A. hydrophila caseinolytic and elastolytic protease, but had no effect on biofilm formation. In vivo challenge test with A. hydrophila showed better survival rate and fish condition in fish groups that were supplemented with M. calabura extract.

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Vanillin inhibits PqsR-mediated virulence in Pseudomonas aeruginosa

Abstract:
Vanillin, a natural phenolic aldehyde from vanilla bean, has been reported to inhibit pqs quorum sensing in Pseudomonas aeruginosa, with potential applications in combinatorial antimicrobial therapy against biofilm infections.

Cited by 47 publications

References 58 publications

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

Vanillin: a review on the therapeutic prospects of a popular flavouring molecule

Screening of some Indonesian medicinal plant extracts for anti quorum sensing activity to prevent Aeromonas hydrophila infection in Oreochromis niloticus

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Vanillin inhibits PqsR-mediated virulence in Pseudomonas aeruginosa

Abstract: Vanillin, a natural phenolic aldehyde from vanilla bean, has been reported to inhibit pqs quorum sensing in Pseudomonas aeruginosa, with potential applications in combinatorial antimicrobial therapy against biofilm infections.

Cited by 47 publications

References 58 publications

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

Vanillin: a review on the therapeutic prospects of a popular flavouring molecule

Screening of some Indonesian medicinal plant extracts for anti quorum sensing activity to prevent Aeromonas hydrophila infection in Oreochromis niloticus

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Abstract:
Vanillin, a natural phenolic aldehyde from vanilla bean, has been reported to inhibit pqs quorum sensing in Pseudomonas aeruginosa, with potential applications in combinatorial antimicrobial therapy against biofilm infections.