Variability of Polygalacturonase and Protein Isoelectric Focusing Patterns in Botrytis cinerea Isolates

Magro, P.; Lenna, P. Di; Marciano, P.; Pallavicini, C.

doi:10.1099/00221287-120-1-105

Cited by 8 publications

(13 citation statements)

References 6 publications

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“…The PG isozymes present significant differences in substrate specificity, kinetic parameters and optimum pH [56,57]. The polygalacturonases isozymes produced by the phytopathogenic fungus Botrytis cinerea have been isolated by using different fermentation conditions [58][59][60][61][62][63][64].…”

Section: Microbial Pectinasesmentioning

confidence: 99%

Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes

Pedrolli¹,

Monteiro²,

Gomes³

et al. 2009

TOBIOTJ

287

123

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Pectinases are a big group of enzymes that break down pectic polysaccharides of plant tissues into simpler molecules like galacturonic acids. It has long been used to increase yields and clarity of fruit juices. Since pectic substances are a very complex macromolecule group, various pectinolytic enzymes are required to degrade it completely. These enzymes present differences in their cleavage mode and specificity being basically classified into two main groups that act on pectin "smooth" regions or on pectin "hairy" regions. Pectinases are one of the most widely distributed enzymes in bacteria, fungi and plants. This review describes the pectinolytic enzymes and their substrates, the microbial pectinase production and characterization, and the industrial application of these enzymes.

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Section: Microbial Pectinasesmentioning

confidence: 99%

Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes

Pedrolli¹,

Monteiro²,

Gomes³

et al. 2009

TOBIOTJ

287

123

View full text Add to dashboard Cite

show abstract

“…Isolate PP, by contrast, showed the presence of a PG peak with PI 8.8 in extracts of both tissues, this difference probably reflecting the variability of the pathogen (cf. Fielding, 1981) particularly as all three isolates originated from vines in a single Italian region (Magro et al, 1980). With Monilinia Zaxa grown in vitro, by contrast, Willetts et al (1977) noted differences in pectin lyase patterns only among Australian isolates, and Fielding (1981) reported little variation in the major PG in extracts of tissue infected by M. laxa.…”

Section: Resultsmentioning

confidence: 96%

“…2 . 1 .15) by the fungus (Zalewska-Sobczak & Urbanek, 1975 ;Drawert & Krefft, 1978;Magro et al, 1980;Fielding, 1981). The fungus is recognized as having considerable natural phenotypic variability (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Multiple Forms of Polygalacturonase in Apple and Carrot Tissue Infected by Isolates of Botrytis cinerea

Lenna

Fielding

1983

Microbiology

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Isoelectric focusing of extracts of carrot root and apple fruit parenchyma infected by each of three isolates of Botrytis cinerea showed the presence of forms of endopolygalacturonase (PG) with isoelectric points (PI) of about 4.5 (a minor peak) and 8-3-8-8. One isolate gave the hitherto unreported peak of pI 8.8 in both tissues, whereas the other two isolates gave a peak of 8.3. The molecular weights of the PGs of pI 8-3-8.8 were estimated to be about 30000. The variation in PI probably reflects the variability of B. cinerea. No evidence of specific enzymic adaptation to host tissues was shown by the ability of PGs to cause changes in permeability of carrot and apple parenchyma.

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“…2000). Polygalacturonases (PGs) in particular are the first cell wall‐degrading enzymes produced by B. cinerea (Cabanne and Doneche 2002), and PG activity may be composed of many isoenzymes (Magro et al. 1980; Leone et al.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Polygalacturonase Enzyme Production in Isolates of Botrytis cinerea Sensitive to Different Fungicides Used in Vineyards

Özer

Köycü

2010

Journal of Phytopathology

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Reduction of mycelial growth and spore germination of Botrytis cinerea, the causal agent of grey mould, are common criteria to determine the sensitivity of this fungus to fungicides. In this article, the influence of nine different fungicides on polygalacturonase (PG) activity and mycelial dry weight (MDW) was analysed on culture filtrates from B. cinerea, obtained from grapes. All fungicides except triadimenol and tebuconazole inhibited MDW of isolates <50%. Cyprodinil + fludioxonil, myclobutanil and imazalil inhibited PG activity more than 50%. Fenhexamid had a lower inhibitory effect (<50%) on PG activity. Procymidone and pyrimethanil induced both PG activity and isoenzyme banding profile of isolates sensitive to these fungicides. This study provides a new additional tool for determining sensitivity to fungicides and monitoring the effect of fungicide resistance management policies.

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Variability of Polygalacturonase and Protein Isoelectric Focusing Patterns in Botrytis cinerea Isolates

Cited by 8 publications

References 6 publications

Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes

Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes

Multiple Forms of Polygalacturonase in Apple and Carrot Tissue Infected by Isolates of Botrytis cinerea

Analysis of Polygalacturonase Enzyme Production in Isolates of Botrytis cinerea Sensitive to Different Fungicides Used in Vineyards

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