Variasi Genetik Hasil Persilangan Nila Best Dengan Red Nifi Dan Nirwana Menggunakan Penanda Rapd

Iskandariah, Iskandariah; Kusmini, Irin Iriana; Arifin, Otong Zenal; Gustiano, Rudhy

doi:10.15578/jra.6.3.2011.363-371

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…RAPD fingerprinting of six populations of giant freshwater prawn M. rosenbergii was reported with polymorphisms of DNA fragment ranging from 29%-76% (Imron et al, 2009), nilem fish Osteochilus hasselti from six populations with level polymorphism of 40%-68% (Mulyasari et al, 2010). While, the low level of polymorphism (18.2%-42.4%) was detected by RAPD marker in tilapia produced from cross-breeding between strains of tilapia (Iskandariah et al, 2011).…”

Section: (Andi Parenrengi)mentioning

confidence: 94%

“…In contrast to isozymes, RAPD provides a more arbitrary sample of the genome and generates essentially unlimited numbers of loci for use in genetic analysis (Fritsch & Rieseberg, 1996). Genetic differentiation based on RAPD analysis in various fish species has been noted in many studies (Bielawski & Pumo, 1997;Coccone et al, 1997;Koh et al, 1999;Liu et al, 1999;Imron et al, 2009;Mulyasari et al, 2010, Moria et al, 2010Imron et al, 2010;Iskandariah et al, 2011;Lante et al, 2011;Nugroho et al, 2011). But so far, no published data on genetic variation of the populations of flying fish H. oxycephalus from Makassar Strait, Indonesia.…”

Section: Introductionmentioning

confidence: 99%

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GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT

Parenrengi

Tenriulo

Ali

2016

Indonesian Aquaculture Journal

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Flying fish, Hirundichthy oxycephalus is one of economically important marine species to Indonesia, particularly in Makassar Strait and Flores Sea. However, there is a limited published data on genetic variation in molecular marker level of this species. Random Amplified Polymorphic DNA (RAPD) was employed in this study to determine the genetic variability of three populations of flying fish collected from Takalar, Pare-Pare, and Majene in Makassar Strait. Genomic DNA was isolated from preserved muscle tissue using phenol-chloroform technique. Two selected arbitrary primers (CA-01 and P-40) were performed to generate RAPD finger printing of flying fish populations. The two primers generated a total of 81 fragments (loci) and 50 polymorphic fragments with size ranging from 125 to 1,250 bp. There were no significant differences in number of fragment and number of polymorphic fragment among populations. The high polymorphism (63.5±7.4%) was obtained from Takalar population followed by Pare-Pare (58.3±19.6%) and Majene population (57.7±0.8%). Similarity index of individuals was 0.60±0.17 for Takalar, 0.63±0.17 for Majene and 0.75±0.21 for Pare-Pare population. Seven fragments were identified as species-specific markers of H. oxycephalus. The UPGMA cluster analysis showed that the Takalar population was genetically closer to Pare-Pare population (D= 0.0812) than to Majene population (D= 0.1873).

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Section: (Andi Parenrengi)mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT

Parenrengi

Tenriulo

Ali

2016

Indonesian Aquaculture Journal

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“…In contrast to isozymes, RAPD provides a more arbitrary sample of the genome and generates essentially unlimited numbers of loci for use in genetic analysis (Fritsch and Rieseberg 1996). Genetic differentiation based on RAPD analysis in various fish species has been noted in many studies (Bielawski and Pumo 1997;Coccone et al 1997;Koh et al 1999;Liu et al 1999;Imron et al 2009;Imron et al 2010;Moria et al 2010;Mulyasari et al 2010;Iskandariah et al 2011;Lante et al 2011;Nugroho et al 2011).…”

Section: Introductionmentioning

confidence: 93%

Genetic variations of Cheilopogon nigricans in the Makassar Strait, Indonesia

Indrayani

Findra

Jufri

et al. 2021

Indo Pac J Ocean Life

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Abstract. Indrayani I, Findra MN, Jufri A, Hidayat H, Pariakan A. 2021. Genetic variations of Cheilopogon nigricans in the Makassar Strait, Indonesia. Indo Pac J Ocean Life 5: 22-28. This study reports DNA Barcoding results (sequencing of cox 1 mitochondrial gene fragments) of four Makassar Strait flying fish species belonging to the Exocoetidae family. Sampling was collected from around the Makassar Strait waters in West Sulawesi. This research was carried out by molecular identification using DNA barcoding of the cytochrome oxidase 1 (COI) gene, the Wizard Promega CO1 primer kit. The molecular identification results showed that the collected fish had 100% and 99.10% genetic similarities with the species Cheilopogon nigricans from the South China Sea. The genetic variations of flying fish in the Makassar Strait are in the same group as flying fish originating from the South China Sea.

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“…The activity of crossing between species of fish that is still in one species results in genetic traits passed down from the parent to the offspring experiencing degradation or decline in genetic quality [11][12][13][14]. To prevent the decline of dominant genes where the dominant genes will be reduced, it is necessary to do hybridization efforts that are able to produce new individuals that have similarities with the parent, in terms of the tilapia (Oreochromis niloticus) nirwana strain, the body shape is relatively wider with a shorter head length, so this makes it has a thicker meat structure compared to other tilapia [15,16].…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Tilapia (Oreochromis niloticus) Nirwana Strain Cultured in Aceh Besar and Wanayasa by Using Random Amplified Polymorphic DNA Method

Yustiati

Hutagalung

Andriani

et al. 2020

AJBGMB

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This research was carried out from June to November 2017. This research aims to analyze genetic relationship of tilapia (Oreochromis niloticus) nirwana strain which is cultured in Aceh Besar (Aceh Province) and in Wanayasa (West Java Province) with RAPD-PCR technique. The DNA Isolation Activity of tilapia nirwana strain was carried out in The Biotechnology Laboratory, Building No.4, FPIK UNPAD, Indonesia and the PCR process was carried out in The Microbiology and Biotechnology Laboratory, Building No.3 FPIK UNPAD, Indonesia. The purpose of this research is to determine the level of genetic relationship of tilapia nirwana strain cultured in Aceh Besar and Wanayasa and to find out whether tilapia nirwana strain in Aceh Besar can be used as a genetic stock. The results showed that genetic relationship level of tilapia nirwana strain cultured in Aceh Besar with those cultured in Wanayasa is 67%. The existence of environmental differences results in genetic diversity between Aceh Besar and Wanayasa tilapia nirwana strain. Factors that causing grouping movement are external factors such as: Geographical isolation and habitat fragmentation as well as internal factors such as mutation, natural selection, genetic drift, and gene flow. Tilapia nirwana strain that is cultured in Aceh Besar produce polymorphic bands, so it can be used as a new genetic stock to stabilize the tilapia nirwana strain production.

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Variasi Genetik Hasil Persilangan Nila Best Dengan Red Nifi Dan Nirwana Menggunakan Penanda Rapd

Cited by 3 publications

References 3 publications

GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT

GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT

Genetic variations of Cheilopogon nigricans in the Makassar Strait, Indonesia

Genetic Analysis of Tilapia (Oreochromis niloticus) Nirwana Strain Cultured in Aceh Besar and Wanayasa by Using Random Amplified Polymorphic DNA Method

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