Genetic Analysis of Tilapia (Oreochromis niloticus) Nirwana Strain Cultured in Aceh Besar and Wanayasa by Using Random Amplified Polymorphic DNA Method

Yustiati, Ayi; Hutagalung, Reifolnanda Mangappu; Andriani, Yuli; Lili, Walim; Bangkit, Ibnu

doi:10.9734/ajbgmb/2020/v4i430111

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…In contrast, low levels of population polymorphism are thought to be related to a constraint on gene flow by environmental factors or limited population size, leading to interpopulation, thereby reducing the population's genetic diversity. [21].…”

Section: A Rapd (Random Amplified Polymorphic Dna) Amplificationmentioning

confidence: 99%

Genetic Variability Analysis of Phaius spp Orchid based on RAPD Markers

Hartati¹,

Samanhudi²

2022

International Journal on Advanced Science, Engineering and Information Technology

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Phaius spp live in tropical forests with high humidity due to deforestation, which has resulted in the extinction of many endemic orchid species, especially orchids Phaius spp, so it needs to be conserved through a plant breeding program. There are not many research reports on the genetic diversity of orchids Phaius spp, even though the study of genetic diversity plays an important role in plant breeding programs and genetic conversation. This study aims to determine the genetic diversity of Phaius spp. This research was carried out using molecular markers RAPD (Random Amplified Polymorphic DNA). DNA from Phaius spp was extracted and then amplified by PCR using 6 RAPD primers. The results of this study found that four species of orchids of the genus Phaius spp (Phaius tankervillae (1); Phaius montanus (2); Phaius collasus (3); and Phaius amboinensis (4)) amplified using 6 RAPD primers to produce 37 DNA bands with a size of 250-1600 bp, and produce 100% polymorphic bands The genetic similarity coefficient of the 4 orchid species ranges from 0.19-0.53 and the genetic variability ranges from 47%-81%.

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Section: A Rapd (Random Amplified Polymorphic Dna) Amplificationmentioning

confidence: 99%

Genetic Variability Analysis of Phaius spp Orchid based on RAPD Markers

Hartati¹,

Samanhudi²

2022

International Journal on Advanced Science, Engineering and Information Technology

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“…. This is reinforced by [13] that the presence of genetic variations or the appearance of polymorphic bands could give rise to new genetic structures in subsequent individuals.…”

Section: Dna Isolationmentioning

confidence: 99%

Genetic Relationship of Four Strains of Guppy (Poeciliareticulata Peters, 1859) Using RAPD-PCR Method

Nuradha

Yustiati

Handaka

et al. 2021

AJBGMB

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This study aims to determine the genetic relationship between four strains of guppy, albino full platinum (AFP), albino german yellow (AGY), top sword (TS) and guppy yellow cobra (GYC) using the RAPD-PCR method. This study used explorative method without experimental design and analyzed by descriptive qualitative and quantitative. The obtained genetic relationship data could be used as data reference for hybridization between strains of guppy fish that have been researched. The research was conducted in October 2020-April 2021. The three fish samples (AFP, TS and GYC) obtained from fish breeder in Cilengkrang-Bandung and AGY sample obtained from fish breeder in Tanggerang-Banten. Based on the results of amplification using OPA-03 primer (AGTCAGCCAC), four strains of guppy fish showed 30 DNA bands that included polymorphic and monomorphic bands. The AFP strains had 19 monomorphic bands, AGY had 21 DNA bands (20 monomorphic bands and one polymorphic bands), TS had 19 DNA bands (17 monomorphic bands and two polymorphic bands) and GYC had 15 DNA bands (14 monomorphic bands and one polymorphic band). Phylogenetic tree analyzed by NTSys program. It is shown between AFP and AGY strains had 95% relationship index, then between TS and GYC strains had 82% relationship index and between AFP-AGY and TS-GYC had 50% relationship index.

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“…Thus, if between these three strains are crossed, it will produce offspring with a new variation. According to [25], the presence of polymorphic band in fish will produce a new genetic structure in fish species.…”

Section: Genetic Relationship Analysismentioning

confidence: 99%

Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR

Hafidah

Yustiati

Mulyani

et al. 2021

AJBGMB

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This research aims to determine genetic diversity of four strains guppy, respectively are japan blue double sword (JBD), japan blue tiger double sword (JBTD), blue moscow (BM), and panda guppy (PG) with RAPD-PCR method. The obtained genetic diversity data is used as guide reference for hybridization between four strains. The research was conducted in September 2020 to April 2021 with explorative methods and in qualitative and quantitative descriptive analysis. The research was carried out in biotechnology Laboratory, Fishery and Marine Sciences Faculty and Central Laboratory,Padjadjaran University, Indonesia.Strains of JBD, JBTD, BM obtained fromCilengkrangSubdistrict, Bandung and PG strain obtained from Parung market, Bogor. Primary OPA-03 (AGTCAGCCAC) is used for standard parameters to interpret genetic diversity among four strains of guppy. Based on results, amplification with OPA-03 primary visualize 25 bands that include five polymorphic bands and 20 monomorphic bands. The phylogenetic tree result show that there are two relationship groups. The first group are JBD, JBTD, and BM with similarity index in the range of 80-89%The first group consist two sub groups of relationship. The first sub group are JBD and JBTD with similarity index of 89%. The second sub group is BM with similarity index of 80%. The second group isPG with similarity index of 65.5%.

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Genetic Analysis of Tilapia (Oreochromis niloticus) Nirwana Strain Cultured in Aceh Besar and Wanayasa by Using Random Amplified Polymorphic DNA Method

Cited by 4 publications

References 6 publications

Genetic Variability Analysis of Phaius spp Orchid based on RAPD Markers

Genetic Variability Analysis of Phaius spp Orchid based on RAPD Markers

Genetic Relationship of Four Strains of Guppy (Poeciliareticulata Peters, 1859) Using RAPD-PCR Method

Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR

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