Variation in Protein Expression Depending on the Severity of Sleep Apnoea-Hypopnoea Syndrome

Gámez, Bernabé Jurado; Moreno, José Luis Gómez-Chaparro; Calero, María Muñoz; Ruiz-Laguna, Julia; Cabrera, Luís Muñoz; Povedano, Andrés Cosano; López-Barea, Juán

doi:10.1016/s1579-2129(10)70070-7

Cited by 3 publications

(7 citation statements)

References 27 publications

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“…Protein expression changes were anticipated in patients with OSA based on our previous study using 2D‐PAGE (Jurado‐Gamez et al. , 2010).…”

Section: Discussionmentioning

confidence: 99%

“…A prospective observational study with a consecutive sample and a control group was carried out in a University Hospital Sleep Unit. Part of the study methodology has been described previously (Jurado‐Gamez et al. , 2010).…”

Section: Methodsmentioning

confidence: 99%

“…To minimize any variance in findings that may be accounted for gender as a confounder, the present study included only men referred to the Sleep Unit, particularly considering that OSA is more frequent in men. Individuals aged between 30 and 65 years, with clinical symptoms and signs compatible with clinical OSA suspicion, were included in this study, and underwent a complete overnight polysomnographic evaluation, as described previously (Jurado‐Gamez et al. , 2010).…”

Section: Methodsmentioning

confidence: 99%

“…, 2009). Indeed, we have previously identified three proteins that showed increased expression in the serum of patients with OSA, while seven other proteins showed reduced levels when compared with control subjects, thereby suggesting that proteomic approaches are worthy of further exploratory efforts (Jurado‐Gamez et al. , 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Among such techniques, the combination of bidimensional electrophoresis [2D-polyacrylamide gel electrophoresis (PAGE)] and mass spectrometry has been extensively used, and has yielded useful insights into various disease processes (Gray et al, 2008;Lee et al, 2009). Indeed, we have previously identified three proteins that showed increased expression in the serum of patients with OSA, while seven other proteins showed reduced levels when compared with control subjects, thereby suggesting that proteomic approaches are worthy of further exploratory efforts (Jurado-Gamez et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Serum proteomic changes in adults with obstructive sleep apnoea

Jurado-Gámez

Gomez-Chaparro

Muñoz-Calero

et al. 2011

Journal of Sleep Research

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SUMMARYTo examine whether differentially expressed proteins are present in the serum of patients with obstructive sleep apnoea (OSA), iTRAQ techniques (isobaric tags for relative and absolute quantification) were employed in a prospective study. Individuals were assigned to either a non-OSA control group (apnoea-hypopnoea index, AHI <5) or an OSA group (AHI ‡5). Blood samples were collected, aliquoted and frozen at )80°C. Protein digestion and tagging with iTRAQ4plex â and mass spectrometry analysis was then performed (MALDI TOF ⁄ TOF). Ten male subjects were included in the control group (age = 45 ± 9.7 years) and 30 male patients in the OSA group (age = 45 ± 10.7 years), the latter being then subdivided into three severity groups. A total of 103 proteins were identified with differential levels between patients with OSA and controls. Of these, 11 proteins were underexpressed and 19 were overexpressed in patients with OSA. C4BPA and thrombospondin were underexpressed in all three OSA severity groups. Among the overexpressed proteins, 13 were overexpressed in the mild OSA group, seven in the moderate group and five in the severe group. Analysis of interactions between the identified proteins revealed that protein alterations in OSA are primarily associated with derangements in lipid and vascular metabolic pathways. This study provides initial evidence that differential protein expression occurs in adults with OSA, and that such proteins change according to disease severity, and appear to primarily involve lipid and vascular metabolic pathways.

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“…Protein expression changes were anticipated in patients with OSA based on our previous study using 2D‐PAGE (Jurado‐Gamez et al. , 2010).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Serum proteomic changes in adults with obstructive sleep apnoea

Jurado-Gámez

Gomez-Chaparro

Muñoz-Calero

et al. 2011

Journal of Sleep Research

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Overview of proteomics studies in obstructive sleep apnea

et al. 2015

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Obstructive sleep apnea (OSA) is an underdiagnosed common public health concern causing deleterious effects on metabolic and cardiovascular health. Although much has been learned regarding the pathophysiology and consequences of OSA in the past decades, the molecular mechanisms associated with such processes remain poorly defined. The advanced high-throughput proteomics-based technologies have become a fundamental approach for identifying novel disease mediators as potential diagnostic and therapeutic targets for many diseases, including OSA. Here, we briefly review OSA pathophysiology and the technological advances in proteomics and the first results of its application to address critical issues in the OSA field.

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