Variation of antioxidants and secondary metabolites in nitrogen-deficient barley plants

Kováčik, Jozef; Klejdus, Bořivoj; Babula, Petr; Jarošová, Markéta

doi:10.1016/j.jplph.2013.08.004

Cited by 50 publications

(17 citation statements)

References 30 publications

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“…Chilling-tolerant maize ( Hodges et al, 1997 ; Taka, 2004 ), cucumber ( Kang and Saltveit, 2002 ), and rice ( Huang and Guo, 2005 ) cultivars had higher antioxidation activities than the sensitive cultivars. Likewise, the enhanced activities of antioxidants protected the plants from oxidative stress caused by the deprivation/deficiency of N ( Tewari et al, 2007 ; Kováčik et al, 2014 ), P ( Tewari et al, 2004 , 2007 ), or K ( Tewari et al, 2007 ; Hafsi et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Seed Priming Alters the Production and Detoxification of Reactive Oxygen Intermediates in Rice Seedlings Grown under Sub-optimal Temperature and Nutrient Supply

et al. 2016

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The production and detoxification of reactive oxygen intermediates (ROIs) play an important role in the plant response to nutrient and environmental stresses. The present study demonstrated the behavior of growth, ROIs-production and their detoxification in primed and non-primed rice seedlings under chilling stress (18°C) and nitrogen-(N), phosphorus-(P), or potassium-(K) deprivation. The results revealed that chilling stress as well as deprivation of any mineral nutrient severely hampered the seedling growth of rice, however, seed priming treatments (particularly selenium- or salicylic acid-priming), were effective in enhancing the rice growth under stress conditions. The N-deprivation caused the maximum reduction in shoot growth, while the root growth was only decreased by P- or K-deprivation. Although, N-deprivation enhanced the root length of rice, the root fresh weight was unaffected. Rate of lipid peroxidation as well as the production of ROIs, was generally increased under stress conditions; the K-deprived seedlings recorded significantly lower production of ROIs than N- or P-deprived seedlings. The responses of enzymatic and non-enzymatic antioxidants in rice seedlings to chilling stress were variable with nutrient management regime. All the seed priming were found to trigger or at least maintain the antioxidant defense system of rice seedlings. Notably, the levels of ROIs were significantly reduced by seed priming treatments, which were concomitant with the activities of ROIs-producing enzymes (monoamine oxidase and xanthine oxidase), under all studied conditions. Based on these findings, we put forward the hypothesis that along with role of ROIs-scavenging enzymes, the greater tolerance of primed rice seedlings can also be due to the reduced activity of ROIs-producing enzymes.

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Section: Introductionmentioning

confidence: 99%

Seed Priming Alters the Production and Detoxification of Reactive Oxygen Intermediates in Rice Seedlings Grown under Sub-optimal Temperature and Nutrient Supply

et al. 2016

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“…This is probably explained by the fact that 2–3 days of LN treatment, as was the case in the present study, is adequate to stimulate the synthesis of phenolic compounds in lettuce. This increased production of phenolic compounds probably occurs via a mechanism that enables plants to resist the oxidative stress resulting from nitrogen deficiency …”

Section: Discussionmentioning

confidence: 99%

“…This increased production of phenolic compounds probably occurs via a mechanism that enables plants to resist the oxidative stress resulting from nitrogen deficiency. 31,32 Phenolic compounds have also been proved to counteract the harmful effects of excess ROS in the human body, which is closely linked to oxidative stress and eventually causes persistent or reversible cellular damage. 3,33 In the present study, the viability of Caco-2 cells decreased in a dose-dependent manner after treatment with H 2 O 2 for 6 h (Fig.…”

Section: Discussionmentioning

confidence: 99%

Reduced nitrogen supply enhances the cellular antioxidant potential of phenolic extracts through alteration of the phenolic composition in lettuce (Lactuca sativa L.)

Zhou

Liang

Zhang³

et al. 2019

J Sci Food Agric

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BACKGROUND Nitrogen availability is an important environmental factor that determines the production of phenolic compounds in vegetables, but the relationship between low nitrogen‐induced alterations of phenolic compounds in vegetable crops and the cellular antioxidant activities of these compounds remains unclear. This study investigated the effect of reduced nitrogen supply (0.05 mmol L−1 nitrate) on phenolic metabolism in lettuce and the protective role of phenolic extracts against H2O2‐induced oxidative stress in Caco‐2 cells by determining cell damage, reactive oxygen species (ROS) content and antioxidant enzyme activities. RESULTS Reduced nitrogen supply significantly improved the accumulation of phenolic compounds in lettuce, which was partially correlated with the upregulation of genes related to the phenolic synthesis pathway. Phenolic extracts from lettuce cultivated in low‐nitrogen medium exhibited a better protective effect against H2O2‐induced oxidative damage in Caco‐2 cells than those from lettuce cultivated with adequate nitrogen. These extracts act by increasing the activities of antioxidant enzymes and, subsequently, by inhibiting ROS overproduction, which leads to a decrease in mitochondrial membrane and DNA damage. The results of HPLC and correlation analyses implied that the improvement in the protective capacity of lettuce extracts after low‐nitrogen treatment may be related, not only to the increased content of phenolic compounds, but also to the increased percentage contribution of chlorogenic acid and quercetin derivatives to the total phenolic content. CONCLUSION Reduction in nitrogen supply can be a powerful strategy to modify phenolic metabolism and composition in lettuce and, consequently, to improve their antioxidant capacity. © 2019 Society of Chemical Industry

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“…Pathogen-induced expression of the nitrate transporter NRT2.6 was also correlated with ROS accumulation ( Dechorgnat et al, 2012 ). Concentrations of antioxidant molecules such as glutathione (GSH) were altered (decreased in shoots and increased in roots) in A. thaliana and barley plants exposed to N deficiency ( Kandlbinder et al, 2004 ; Kovacik et al, 2014 ).…”

Section: Nitric Oxide and N Metabolismmentioning

confidence: 99%

Cross-Regulation between N Metabolism and Nitric Oxide (NO) Signaling during Plant Immunity

Thalineau¹,

Truong²,

Berger³

et al. 2016

Front. Plant Sci.

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Plants are sessile organisms that have evolved a complex immune system which helps them cope with pathogen attacks. However, the capacity of a plant to mobilize different defense responses is strongly affected by its physiological status. Nitrogen (N) is a major nutrient that can play an important role in plant immunity by increasing or decreasing plant resistance to pathogens. Although no general rule can be drawn about the effect of N availability and quality on the fate of plant/pathogen interactions, plants’ capacity to acquire, assimilate, allocate N, and maintain amino acid homeostasis appears to partly mediate the effects of N on plant defense. Nitric oxide (NO), one of the products of N metabolism, plays an important role in plant immunity signaling. NO is generated in part through Nitrate Reductase (NR), a key enzyme involved in nitrate assimilation, and its production depends on levels of nitrate/nitrite, NR substrate/product, as well as on L-arginine and polyamine levels. Cross-regulation between NO signaling and N supply/metabolism has been evidenced. NO production can be affected by N supply, and conversely NO appears to regulate nitrate transport and assimilation. Based on this knowledge, we hypothesized that N availability partly controls plant resistance to pathogens by controlling NO homeostasis. Using the Medicago truncatula/Aphanomyces euteiches pathosystem, we showed that NO homeostasis is important for resistance to this oomycete and that N availability impacts NO homeostasis by affecting S-nitrosothiol (SNO) levels and S-nitrosoglutathione reductase activity in roots. These results could therefore explain the increased resistance we noted in N-deprived as compared to N-replete M. truncatula seedlings. They open onto new perspectives for the studies of N/plant defense interactions.

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Variation of antioxidants and secondary metabolites in nitrogen-deficient barley plants

Cited by 50 publications

References 30 publications

Seed Priming Alters the Production and Detoxification of Reactive Oxygen Intermediates in Rice Seedlings Grown under Sub-optimal Temperature and Nutrient Supply

Seed Priming Alters the Production and Detoxification of Reactive Oxygen Intermediates in Rice Seedlings Grown under Sub-optimal Temperature and Nutrient Supply

Reduced nitrogen supply enhances the cellular antioxidant potential of phenolic extracts through alteration of the phenolic composition in lettuce (Lactuca sativa L.)

Cross-Regulation between N Metabolism and Nitric Oxide (NO) Signaling during Plant Immunity

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