Vascular toxicity of ultra-small TiO2 nanoparticles and single walled carbon nanotubes in vitro and in vivo

Bayat, Narges; Lopes, Viviana R.; Schölermann, Julia; Jensen, Lasse D.; Cristóbal, Susana

doi:10.1016/j.biomaterials.2015.05.044

Cited by 62 publications

(33 citation statements)

References 64 publications

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“…The specific surface area of Cu NPs and Cu MPs was measured as 14.7 and 0.16 m 2 /g, respectively using the Brunauer-Emmett-Teller (BET) method. Suspension stability and surface charge reflect the interaction of NPs with physiological milieus because of their large surface area/volume ratio [32, 33]. The zeta potential of the Cu NPs was 25.5 ± 0.8 mV for pH 1.5, 1.32 ± 1.2 mV for pH 6.8, and -6.2 ± 0.2 mV for pH 7.8.…”

Section: Resultsmentioning

confidence: 99%

Comparative toxicity and biodistribution assessments in rats following subchronic oral exposure to copper nanoparticles and microparticles

Lee

Park

et al. 2016

Part Fibre Toxicol

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BackgroundCopper nanoparticles (Cu NPs) have great potential in electronics and biomedical fields because of their efficient thermodynamic and anti-microbial properties. However, their potential toxic effects and kinetic data following repeated exposure are still unclear.MethodsWe evaluated the physicochemical properties of Cu NPs (25 nm) and copper microparticles (Cu MPs, 14–25 μm). Comparative in vivo toxicity of Cu NPs and Cu MPs was evaluated by conducting a 28-day repeated oral dose study at equivalent dose levels of 0, 100, 200, and 400 mg/kg/day (vehicle, 1 % hydroxypropyl methylcellulose). We determined Cu levels in the blood, tissues, urine, and feces by using inductively coupled plasma mass spectrometry.ResultsThe solubility of Cu NPs and Cu MPs was 84.5 and 17.2 %, respectively, in an acidic milieu; however, they scarcely dissolved in vehicle or intestinal milieus. The specific surface area of Cu NPs and Cu MPs was determined to be 14.7 and 0.16 m2/g, respectively. Cu NPs exhibited a dose-dependent increase of Cu content in the blood and tested organs, with particularly high levels of Cu in the liver, kidney, and spleen. Only for liver and kidney increased Cu levels were found in Cu MPs-treated rats. Cu NPs caused a dose-related increase in Cu levels in urine, whereas Cu MPs did not affect the urine Cu levels. Extremely high levels of Cu were detected in the feces of Cu MPs-treated rats, whereas much lower levels were detected in the feces of Cu NPs-treated rats. A comparative in vivo toxicity study showed that Cu NPs caused damages to red blood cells, thymus, spleen, liver, and kidney at ≥200 mg/kg/days, but Cu MPs did not cause any adverse effects even at the highest dose.ConclusionsOverall, the in vivo repeated dose toxicity study of Cu NPs and Cu MPs demonstrated that large surface area and high solubility in physiological milieus could directly influence the toxicological responses and biodistribution of Cu particles when administered orally. Under these experimental conditions, the no-observed-adverse-effect levels of Cu NPs and Cu MPs were determined to be 100 and ≥400 mg/kg/day, respectively.Electronic supplementary materialThe online version of this article (doi:10.1186/s12989-016-0169-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Comparative toxicity and biodistribution assessments in rats following subchronic oral exposure to copper nanoparticles and microparticles

Lee

Park

et al. 2016

Part Fibre Toxicol

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“…Exposure to SWCNTs resulted in mortality, pericardial oedema, and increased levels of myosin 1C (Myo1C), which were associated with glomerular development in zebrafish embryos in vivo , and cytotoxicity and oxidative damage in vitro in a human dermal microvascular endothelial cell line (HMEC‐1). Moreover, decreased NO release and anti‐angiogenic effects were observed in both models …”

Section: Bio‐toxicitymentioning

confidence: 87%

Bio‐effect of nanoparticles in the cardiovascular system

Hong

Zhang

2016

J Biomedical Materials Res

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Nanoparticles (NPs; < 100 nm) are increasingly being applied in various fields due to their unique physicochemical properties. The increase in human exposure to NPs has raised concerns regarding their health and safety profiles. The potential correlation between NP exposure and several cardiovascular (CV) events has been demonstrated. The aim of this review is to provide a comprehensive evaluation of the current knowledge regarding the bio-toxic impacts of titanium oxide, silver, silica, carbon black, carbon nanotube, and zinc oxide NPs exposure on the CV system in terms of in vivo and in vitro experiments, which is not fully understood presently. Moreover, the potential toxic mechanisms of NPs in the CV system that are still being questioned are elaborately discussed, and the underlying capacity of NPs used in medicine for CV events are summarized. It will be an important instrument to extrapolate relevant data for human CV risk evaluation and management. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2881-2897, 2016.

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“…Studies of SWCNTs predominantly show an association between exposure and increased levels of DNA strand breaks in cultured cells (Kisin et al 2007, 2011; Pacurari et al 2008; Yang et al 2009; Lindberg et al 2009, 2013; Migliore et al 2010; Cicchetti et al 2011; Di Giorgio et al 2011; Pelka et al 2013; Alarifi et al 2014; Kim & Yu 2014; Vesterdal et al 2014a), whereas a few studies have shown null effect (Jacobsen et al 2008; Darne et al 2014; Bayat et al 2015). Although these studies clearly show that exposure to CNTs is related with increased levels of DNA strand breaks, an assessment of relevant fiber characteristics responsible for this effect remains inconclusive.…”

Section: Genotoxicitymentioning

confidence: 99%

Evaluating the mechanistic evidence and key data gaps in assessing the potential carcinogenicity of carbon nanotubes and nanofibers in humans

Kuempel

Jaurand

Møller

et al. 2016

Critical Reviews in Toxicology

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In an evaluation of carbon nanotubes (CNTs) for the IARC Monograph 111, the Mechanisms Subgroup was tasked with assessing the strength of evidence on the potential carcinogenicity of CNTs in humans. The mechanistic evidence was considered to be not strong enough to alter the evaluations based on the animal data. In this paper, we provide an extended, in-depth examination of the in vivo and in vitro experimental studies according to current hypotheses on the carcinogenicity of inhaled particles and fibers. We cite additional studies of CNTs that were not available at the time of the IARC meeting in October 2014, and extend our evaluation to include carbon nanofibers (CNFs). Finally, we identify key data gaps and suggest research needs to reduce uncertainty. The focus of this review is on the cancer risk to workers exposed to airborne CNT or CNF during the production and use of these materials. The findings of this review, in general, affirm those of the original evaluation on the inadequate or limited evidence of carcinogenicity for most types of CNTs and CNFs at this time, and possible carcinogenicity of one type of CNT (MWCNT-7). The key evidence gaps to be filled by research include: investigation of possible associations between in vitro and early-stage in vivo events that may be predictive of lung cancer or mesothelioma, and systematic analysis of dose–response relationships across materials, including evaluation of the influence of physico-chemical properties and experimental factors on the observation of nonmalignant and malignant endpoints.

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Vascular toxicity of ultra-small TiO2 nanoparticles and single walled carbon nanotubes in vitro and in vivo

Cited by 62 publications

References 64 publications

Comparative toxicity and biodistribution assessments in rats following subchronic oral exposure to copper nanoparticles and microparticles

Comparative toxicity and biodistribution assessments in rats following subchronic oral exposure to copper nanoparticles and microparticles

Bio‐effect of nanoparticles in the cardiovascular system

Evaluating the mechanistic evidence and key data gaps in assessing the potential carcinogenicity of carbon nanotubes and nanofibers in humans

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