Vascularization of iNSC spheroid in a 3D spheroid‐on‐a‐chip platform enhances neural maturation

Shin, Nari; Kim, Youngtaek; Ko, Jihoon; Choi, Soon Won; Hyung, Sujin; Lee, SeungEun; Park, Kyoung Sun; Song, Ji-Young; Jeon, Noo Li; Kang, Kyung‐Sun

doi:10.1002/bit.27978

Cited by 27 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shin et al developed a platform to model the 3D vascular networks of the brain-on-a-chip. 51 Induced neural stem cells (iNSC) were formed into spheroids and cultured with a vascular network. This model of iNSC spheroids and perfused blood vessels was formed in a shape where the vascular network was wrapped around the iNSC spheroid and performed perfusion, and the brain neural stem cells were in direct contact with the blood vessels in vivo .…”

Section: D Multicellular Interaction In Tumour Microenvironmentmentioning

confidence: 99%

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Kim

Cho

2022

Analyst

View full text Add to dashboard Cite

show abstract

Section: D Multicellular Interaction In Tumour Microenvironmentmentioning

confidence: 99%

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Kim

Cho

2022

Analyst

View full text Add to dashboard Cite

show abstract

“…The first method consists of co-culturing neuronal spheroids with perfusable blood vessels. The vascularized neuronal spheres could efficiently enhance proliferation, differentiation, and reduce apoptosis [ 99 ]. The second method is comprised of the separate generation of vessel organoids and brain organoids followed by a co-culture of two types of organoids.…”

Section: Future Perspectivesmentioning

confidence: 99%

Human Brain Organoid: A Versatile Tool for Modeling Neurodegeneration Diseases and for Drug Screening

Seong

et al. 2022

Stem Cells International

View full text Add to dashboard Cite

Clinical trials serve as the fundamental prerequisite for clinical therapy of human disease, which is primarily based on biomedical studies in animal models. Undoubtedly, animal models have made a significant contribution to gaining insight into the developmental and pathophysiological understanding of human diseases. However, none of the existing animal models could efficiently simulate the development of human organs and systems due to a lack of spatial information; the discrepancy in genetic, anatomic, and physiological basis between animals and humans limits detailed investigation. Therefore, the translational efficiency of the research outcomes in clinical applications was significantly weakened, especially for some complex, chronic, and intractable diseases. For example, the clinical trials for human fragile X syndrome (FXS) solely based on animal models have failed such as mGluR5 antagonists. To mimic the development of human organs more faithfully and efficiently translate in vitro biomedical studies to clinical trials, extensive attention to organoids derived from stem cells contributes to a deeper understanding of this research. The organoids are a miniaturized version of an organ generated in vitro, partially recapitulating key features of human organ development. As such, the organoids open a novel avenue for in vitro models of human disease, advantageous over the existing animal models. The invention of organoids has brought an innovative breakthrough in regeneration medicine. The organoid-derived human tissues or organs could potentially function as invaluable platforms for biomedical studies, pathological investigation of human diseases, and drug screening. Importantly, the study of regeneration medicine and the development of therapeutic strategies for human diseases could be conducted in a dish, facilitating in vitro analysis and experimentation. Thus far, the pilot breakthrough has been made in the generation of numerous types of organoids representing different human organs. Most of these human organoids have been employed for in vitro biomedical study and drug screening. However, the efficiency and quality of the organoids in recapitulating the development of human organs have been hindered by engineering and conceptual challenges. The efficiency and quality of the organoids are essential for downstream applications. In this article, we highlight the application in the modeling of human neurodegenerative diseases (NDDs) such as FXS, Alzheimer’s disease (AD), Parkinson’s disease (PD), and autistic spectrum disorders (ASD), and organoid-based drug screening. Additionally, challenges and weaknesses especially for limits of the brain organoid models in modeling late onset NDDs such as AD and PD., and future perspectives regarding human brain organoids are addressed.

show abstract

“…In fact, vessel organoids were successfully obtained from induced Pluripotent Stem (iPS) cells, with an appropriate organization of endothelial cells, pericytes, and basement membrane, which were efficiently connected after grafting in mice and perfused [ 115 ]. Remarkably, a 3D model of neural stem cell spheroids could also be efficiently colonized by endothelial vessels, and its proper perfusion was illustrated by the diffusion of microbeads within the whole network [ 116 ]. Interestingly, the presence of vessels improved the maturation of neural stem cells and alleviated their apoptosis rate, thus reinforcing the concept of angiocriny.…”

Section: Future Is Coming Giving a New Dimension To The Tradementioning

confidence: 99%

A Bloody Conspiracy— Blood Vessels and Immune Cells in the Tumor Microenvironment

Terrassoux

Claux

Bacari

et al. 2022

Cancers

View full text Add to dashboard Cite

Cancer progression occurs in concomitance with a profound remodeling of the cellular microenvironment. Far from being a mere passive event, the re-orchestration of interactions between the various cell types surrounding tumors highly contributes to the progression of the latter. Tumors notably recruit and stimulate the sprouting of new blood vessels through a process called neo-angiogenesis. Beyond helping the tumor cope with an increased metabolic demand associated with rapid growth, this also controls the metastatic dissemination of cancer cells and the infiltration of immune cells in the tumor microenvironment. To decipher this critical interplay for the clinical progression of tumors, the research community has developed several valuable models in the last decades. This review offers an overview of the various instrumental solutions currently available, including microfluidic chips, co-culture models, and the recent rise of organoids. We highlight the advantages of each technique and the specific questions they can address to better understand the tumor immuno-angiogenic ecosystem. Finally, we discuss this development field’s fundamental and applied perspectives.

show abstract

Vascularization of iNSC spheroid in a 3D spheroid‐on‐a‐chip platform enhances neural maturation

Cited by 27 publications

References 43 publications

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Human Brain Organoid: A Versatile Tool for Modeling Neurodegeneration Diseases and for Drug Screening

A Bloody Conspiracy— Blood Vessels and Immune Cells in the Tumor Microenvironment

Contact Info

Product

Resources

About