Vector transmission of konjac mosaic virus to calla lily (<i>Zantedeschia</i> spp.) by aphids

Liao, Min‐Ting; Mou, De‐Fen; Chang, Yuan-Chi; Tsai, Chang‐Her

doi:10.1111/aab.12628

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…KoMV isolates in Zantedeschia are reported to be a significant problem in Taiwan, ever since the plant was introduced in the 1990's from New Zealand and the Netherlands, suggesting the virus has been present within commercial Zantedeschia for many years (Liao et al. , 2020). This finding suggests that KoMV is more widely distributed than currently reported.…”

Section: Figurementioning

confidence: 99%

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First report of Konjac mosaic virus in Zantedeschia aethiopica from the United Kingdom

Chisnall

Frew

Hogan

et al. 2022

New Disease Reports

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Konjac mosaic virus, potyvirus, Zantedeschia aethiopica F I G U R E 1 Symptoms on Zantedeschia aethiopica observed in the nursery in Hampshire In June 2019 a sample of lily (Zantedeschia aethiopica) was submitted to Fera Science Ltd from a horticultural retail nursery in Hampshire, England. The sample was sent in following a routine inspection, where the appearance of an unknown disease was discovered. Symptoms manifested as yellow streaks on the leaves (Figs 1-3). In a batch of 60,000 plants, approximately 50% showed symptoms.

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Section: Figurementioning

confidence: 99%

“…KoMV is known to be transmitted by aphids (Liao et al. , 2020) so aphid transmission from other local plants is possible. Alternatively, low levels of the virus may have been present in the parent material, having built up over years of continuous propagation.…”

Section: Figurementioning

confidence: 99%

First report of Konjac mosaic virus in Zantedeschia aethiopica from the United Kingdom

Chisnall

Frew

Hogan

et al. 2022

New Disease Reports

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Potyvirus Infecting Ornamental Plants Grown in the Neotropical Region

Alexandre

Duarte

Chaves

et al. 2021

Virus Diseases of Ornamental Plants

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First Report of Konjac Mosaic Virus in Spuriopimpinella brachycarpa in Korea

et al. 2023

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Spuriopimpinella brachycarpa Nakai (Common name, Chamnamul; family Apiaceae) is a plant whose leaves are consumed as a vegetable and used as a folk medicine in Korea (Kim et al., 2020). In February 2020, seven samples of S. brachycarpa leaf showing virus symptoms including yellowing, vein chlorosis, chlorotic lesions, and severe mottling were collected from a greenhouse in Busan, South Korea, to diagnose the potential disease (Fig. S1a, b). The disease incidence rate in the greenhouse was >10% (2,970 m2). To identify the causal virus, we analyzed leaf dip preparation and thin sections of the symptomatic leaves by transmission electron microscopy. Filamentous virus particles and pinwheel structures were observed, indicating the presence of a potyvirus (Fig. S1c, d). To confirm these results, the symptomatic leaf samples were further analyzed by reverse-transcription polymerase chain reaction (RT-PCR) using potyvirus universal primers (Table S2) and direct sequencing of the PCR products. All samples were positive for konjac mosaic virus (KoMV). To exclude the possibility of infection by multiple viruses, we performed high-throughput sequencing (HTS) on an Illumina NovaSeq 6000 system (Macrogen Inc., Seoul, South Korea). There were two contigs (9,267 and 2,851 nt) mapping to KoMV sequences. A large contig (9,267 nt; 705,967 mapped reads; mean read coverage of 11,351.4x) showed about 80% identity (93% coverage) with KoMV-F (GenBank accession no. NC_007913) isolated from Amorphophallus konjac in Japan (Nishiguchi et al., 2006). To isolate KoMV from S. brachycarpa, we mechanically inoculated leaf extracts from symptomatic samples onto Chenopodium quinoa as an assay host via three single-lesion passages, followed by propagation in Nicotiana benthamiana. In a bioassay of the KoMV isolate (KoMV-BS), we mechanically inoculated sap from infected N. benthamiana onto 31 indicator plants including Cryptotaenia japonica (Apiaceae), which is similar to S. brachycarpa (Table S3). KoMV-BS systemically induced vein chlorosis and/or leaf mottling in four Nicotiana species and C. japonica, and chlorotic local lesions in upper leaves of C. quinoa; no symptoms were observed in 25 other indicator plants. These results were confirmed by RT-PCR. Next, we obtained the complete genome sequence of KoMV-BS using HTS and 5′ and 3′ rapid amplification of cDNA ends, with newly designed primers (Table S2). The assembled full-length KoMV-BS genome sequence was 9,392 nt in length, excluding the poly(A) tail, and encoded a polyprotein composed of 3,060 amino acids. The sequence was deposited in GenBank (accession no. OR001914). BLAST analysis showed 84~88% and 90~98% identities at CP nucleotide and amino acid levels, respectively with the reported KoMV isolates, confirming the virus to be an isolate of KoMV (synonym; Japanese hornwort mosaic virus, zantedeschia mosaic virus) (Adams et al., 2005; Nishiguchi et al., 2006). KoMV infection was first reported in A. konjac from Japan (Shimoyama et al. 1992) and has been spread worldwide as one of the major causal agents of viral diseases in calla lily (Liao et al., 2020). To the best of our knowledge, this is the first report of KoMV infection in S. brachycarpa. To date, cucumber mosaic virus and tobacco mosaic virus have been reported to infect S. brachycarpa in Korea (Yoon et al., 2016; 2017). Our findings will be helpful for developing virus-management strategies to prevent yield and quality loss in S. brachycarpa.

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Vector transmission of konjac mosaic virus to calla lily (Zantedeschia spp.) by aphids

Cited by 3 publications

References 31 publications

First report of Konjac mosaic virus in Zantedeschia aethiopica from the United Kingdom

First report of Konjac mosaic virus in Zantedeschia aethiopica from the United Kingdom

Potyvirus Infecting Ornamental Plants Grown in the Neotropical Region

First Report of Konjac Mosaic Virus in Spuriopimpinella brachycarpa in Korea

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