Vegetative compatibility groups and sexual reproduction among Spanish Monilinia fructicola isolates obtained from peach and nectarine orchards, but not Monilinia laxa

Cal, A. De; Egüen, Beatriz; Melgarejo, P.

doi:10.1016/j.funbio.2014.03.007

Cited by 20 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetic variability may be associated with environmental pressure factors such as the use of fungicides and climatic conditions. Likewise, sexual reproduction is also associated with variability between isolates and can result in adaptability to new environments, hosts or resistance to fungicides (Bosshard et al, 2006;De Cal et al, 2014). Correct species identification and knowledge of genetic diversity will contribute to the understanding of progression and distribution of brown rot and management control.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

et al. 2016

View full text Add to dashboard Cite

Fungi of the Monilinia genus occur worldwide and affect a wide range of economically important stone fruits. Several Monilinia species are responsible for brown rot. Although this disease is common in Brazil, Monilinia sp. genetic variability in Brazilian orchards has generally been poorly characterized. The present study represents the first report on the genetic diversity of Monilinia sp. from Brazilian orchards. The genetic structure of the Brazilian population was also compared to isolates from other countries, together with some morphological characteristics and aggressiveness. Sixty‐one isolates belonging to the Monilinia genus were obtained from different orchards in Brazilian states. Ten Monilinia fructicola isolates from the United States and one isolate from a fruit imported into Brazil were also evaluated. Phylogenetic analysis of the ITS1‐5.8S‐ITS2 region (internal transcribed spacer) clustered most Brazilian and American isolates with M. fructicola authentic strains from Q‐Bank. Two isolates (one from an imported fruit) clustered as Monilinia laxa. The results revealed M. fructicola as the prevalent species associated with brown rot in Brazilian orchards. To evaluate the intraspecific diversity of M. fructicola and M. laxa, multigene sequence analysis was performed using ITS1‐5.8S‐ITS2 and TEF1 (elongation factor 1). Whilst TEF1 is the most phylogenetically informative gene for intraspecific studies of M. fructicola, RPB2 (RNA polymerase II gene) displayed low variation in intraspecific analysis, but was an informative locus for assigning isolates to M. fructicola or M. laxa species. The amova suggests that Brazilian isolates from the States of the main producing regions belong to a single genetic population, which is genetically distinct from the US (Californian) population of M. fructicola.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

et al. 2016

View full text Add to dashboard Cite

show abstract

“…M. fructicola ascospores have not yet been found in any European peach orchards (EFSA 2011), although we have previously reported that the M. fructicola population in the Sudanell orchard (Lleida, Spain) would potentially have a teleomorphic stage (Villarino et al 2012). In another recent work of our group we have identified six vegetative compatibility groups (VCGs) in our study population and we were able to produce apothecia of our M. fructicola population in the laboratory, whereas no different VCGs or apothecia were described for M. laxa (De Cal et al 2014). Finally, the potential of M. fructicola to rapidly develop resistance to fungicides (Michailides et al 1987) led us also to consider the resistance as another factor involved on the species displacement.…”

Section: Introductionmentioning

confidence: 66%

Sensitivity of Monilinia fructicola from Spanish peach orchards to thiophanate-methyl, iprodione, and cyproconazole: fitness analysis and competitiveness

Egüen¹,

Melgarejo²,

Cal³

2014

Eur J Plant Pathol

Self Cite

View full text Add to dashboard Cite

Benzimidazoles, dicarboximides and demethylation inhibitors are the main group of fungicides used to control brown rot in Spain. The causal agents of brown rot in peach (Prunus persica (L.) Batsch) orchards in Spain are Monilinia laxa M. fructigena, and M. fructicola. The dynamics of fungicide sensitivity and fitness of M. fructicola population, the most recent species causing brown rot in the Ebro Valley (Lleida, Spain), were characterized by determining their resistance, fitness, and in vitro competitiveness to thiophanate-methyl (TM), iprodione (I), and cyproconazole (CPZ) in field isolates recovered over the 2006-2010 period. We found that: (a) more than 95 % of the M. fructicola isolates are high TM-resistant, (b) more than 50 % of the M. fructicola isolates are Iresistant and these frequencies of occurrence did not change during our 5-year survey, and (c) a few CPZresistant isolates have been also detected in population since 2008. We identified five different fungicideresistant (R) and/or fungicide-sensitive (S) phenotypes and found that our study population contains multiple fungicide-resistant isolates. Moreover, these fungicideresistant isolates display high parasitic fitness on fruit and flowers and high competitiveness. These findings suggest that the TM, I, and CPZ resistance of M. fructicola isolates could be contributing as another factor on changing the frequency of occurrence of the three Monilinia species in the Ebro Valley.

show abstract

“…Then, M. fructicola was detected for the first time in peach orchards in the Ebro valley, Lerida, Spain [5], and spread, displacing M. laxa, a supposedly indigenous pathogen [56], and reaching the same level of diffusion [56]. Nowadays, in Spain, both Monilinia species (M. laxa (Aderhold and Ruhland) and M. fructicola (G. Winter) Honey) coexist in the field [4,47].…”

Section: Monilinia Sppmentioning

confidence: 99%

“…Egüen et al [18] suggested that fungicide resistance of the M. fructicola population is co-acting with other factors such as an adaptation in the pathogen, changing the frequency of occurrence of the three Monilinia species in Spain. The displacement of M. laxa by M. fructicola in Spain has also been attributed to its conjugational potency related to sexual exchange and ability to produce ascopores from pseudosclerotial mummified fruits and their gradual process of a sexual propagation [56]. M. laxa is not known to produce apothecia [56], while M. fructicola does, from which ascopores can easily be disseminated in the spring for possible infection in the fruiting season [58].…”

Section: Monilinia Sppmentioning

confidence: 99%

Peach Brown Rot: Still in Search of an Ideal Management Option

2018

View full text Add to dashboard Cite

Abstract:The peach is one of the most important global tree crops within the economically important Rosaceae family. The crop is threatened by numerous pests and diseases, especially fungal pathogens, in the field, in transit, and in the store. More than 50% of the global post-harvest loss has been ascribed to brown rot disease, especially in peach late-ripening varieties. In recent years, the disease has been so manifest in the orchards that some stone fruits were abandoned before harvest. In Spain, particularly, the disease has been associated with well over 60% of fruit loss after harvest. The most common management options available for the control of this disease involve agronomical, chemical, biological, and physical approaches. However, the effects of biochemical fungicides (biological and conventional fungicides), on the environment, human health, and strain fungicide resistance, tend to revise these control strategies. This review aims to comprehensively compile the information currently available on the species of the fungus Monilinia, which causes brown rot in peach, and the available options to control the disease. The breeding for brown rot-resistant varieties remains an ideal management option for brown rot disease control, considering the uniqueness of its sustainability in the chain of crop production.

show abstract

Vegetative compatibility groups and sexual reproduction among Spanish Monilinia fructicola isolates obtained from peach and nectarine orchards, but not Monilinia laxa

Cited by 20 publications

References 33 publications

Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

Characterization of Monilinia species associated with brown rot in stone fruit in Brazil

Sensitivity of Monilinia fructicola from Spanish peach orchards to thiophanate-methyl, iprodione, and cyproconazole: fitness analysis and competitiveness

Peach Brown Rot: Still in Search of an Ideal Management Option

Contact Info

Product

Resources

About