Veränderungen der Aktivität einiger Enzyme im Weizenblatt in Abhängigkeit von der temperaturlabilen Verträglichkeit für <i>Puccinia graminis tritici</i><sup>1)</sup>

Frič, F.; Fuchs, W. H.

doi:10.1111/j.1439-0434.1970.tb02457.x

Cited by 44 publications

(19 citation statements)

References 29 publications

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“…Peroxidase (G-POX; EC 1.11.1.7) activity was determined spectrophotometrically (Jenway 6400, Krackeler Scientific, London, UK) based on the oxidation of guaiacol in the presence of H2O2 at 440 nm (Frič & Fuchs 1970). The assay mixture contained 0.1 M Na-acetate buffer (pH 5.2), 5 mM guaiacol, 8 mM H2O2, and 0.1 mL of supernatant.…”

Section: Enzymes Activitiesmentioning

confidence: 99%

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

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Abstract:The seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.

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Section: Enzymes Activitiesmentioning

confidence: 99%

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

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“…The specific enzyme activity was expressed as the enzyme unit per mg of soluble protein (U mg −1 SP). Guaiacol peroxidase (GPX) activity was measured spectrophotometrically based on the oxidation of guaiacol in the presence of H2O2 at 440 nm according to the method of Frič & Fuchs (1970). Specific GPX activity were expressed as enzymatic activity unit min…”

Section: Determination Of Antioxidant Enzymes Activity In Rootsmentioning

confidence: 99%

Effect of silicon application on Sorghum bicolor exposed to toxic concentration of zinc

et al. 2012

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The aim of this study was to determine the effect of exogenously applied Si on the growth and physiological parameters of sorghum [Sorghum bicolor (L.) Moench] cultivated in hydroponics with elevated zinc concentrations (75 µM and 150 µM Zn). Increased concentrations of Zn inhibited root growth and biomass production of roots and shoots. Application of Si individually showed a positive effect on root growth but negatively affected production of fresh and dry biomass of roots and shoots. On the other hand, silicon in combination with Zn significantly reduced the inhibitory effect of Zn on root growth but did not positively affect biomass production of roots and shoots. Accumulation of Zn in plant tissues increased with increasing Zn concentration in nutrient solution, but application of Si in combination with Zn did not significantly influence Zn accumulation in roots. Completely opposite results were found in Si accumulation in roots treated with Si in combination with Zn. Interaction of these ions resulted in considerable increase of Si accumulation in roots which almost doubled in comparison to individal Si treatment. Impact of Zn on the activity of some antioxidant enzymes was equivocal and differences were observed also between two Zn concentrations. Individual application of Si resulted in significant increase in the activity of all studied antioxidant enzymes but Si in combination with Zn mostly negatively affected their activity except the activity of catalase (CAT) which was the highest in roots grown in solution containing both Si and Zn ions. Comparing all obtained data we can assume that Si applied in combination with Zn did not significantly alleviate Zn toxicity in young sorghum except the growth of primary seminal root and further experiments are required for better understanding of their interaction.

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“…Abbreviations: BSA, bovine serum albumin; CAD, ei in/ "' 'l^hydrogenase; DTT, dithiothreitol; Pgt, Pucci p. tritici, PO, peroxidases; TMPD, N,N,N', nnamylia gram-N'-tetrahenylenediamine; Tris, 2-amino-2-hydroxymethylmany. Fuchs, 1970;Daly, Ludden & Seevers, 1971;Seevers, Daly & Gatedral, 1971 ;Johnson & Gunningham, 1972;Thorpe & Hall, 1984). Generally, resistant plants exhibited higher pet-oxidase activities during infection than did susceptible ones.…”

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confidence: 99%

Peroxidase isoenzyme patterns of resistant and susceptible wheat leaves following stem rust infection

1989

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Near-isogenic lines of the wheat {Triticutn aestivum L.) cultivar Prelude, carrying eitber tbe sr5 gene for susceptibility or tbe Sr5 allele for resistance to stem rust {Puccinia gratttinis Pers. f.sp. tritici Erics. & E. Henn.) ' •^' ere inoculated witb race 32 of tbe fungus wbicb possesses tbe P5 gene for-avirulence. Enzyme extracts prepared 'It different times after inoculation were separated by non-denaturing gel electropboresis and tbe gels were stained '"'-peroxidase acti\-ity using different substrates. Botb anodic and cathodic gels were run.In anodic separations, resistant and susceptible plants initially botb exbibited decreases in tbe activity of two 'soenzymes of low-molecular mass, increases in two enzymes of intermediate molecular mass, and induction of two new bigb molecular mass isoperoxidases. In susceptible plants, tbese changes proceeded until 3 days after 'noculation, when a general decrease in all isoenzymes was observed until 8 days after inoculation. In contrast, in plants, tbe increases continued up to 7 days after inoculation. In addition, one isoperoxidase appeared 2-3 days after inoculation only in tbe incompatible interaction.lie separations revealed tbat l:)asic isoperoxidases increased after inoculation as well. In tbe group of small 8S1C isoenzymes, two new bands appeared following inoculation. Only slight differences were seen between tbe **o isolines.Injecting an elicitor isolated from germinated urediniospores into tbe intercellular spaces of wbeat lea\-es induces Epical symptoms of tbe resistant reaction in botb isolines. Correspondingly, tbe isoperoxidase pattern of tbê compatible interaction, including tbe resistance related isoenzyme, was induced in botb isolines. I he results are compared witb previously reported cbanges in enzyme activities of tbe lignin biosyntbetic Pathway, including tbe total activity of peroxidase. Tbe relationship of different isoenzymes to tbe lignification sponse as a decisive mechanism of the hypersensitive resistant reaction is discussed.

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Veränderungen der Aktivität einiger Enzyme im Weizenblatt in Abhängigkeit von der temperaturlabilen Verträglichkeit für Puccinia graminis tritici¹⁾

Cited by 44 publications

References 29 publications

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Effect of silicon application on Sorghum bicolor exposed to toxic concentration of zinc

Peroxidase isoenzyme patterns of resistant and susceptible wheat leaves following stem rust infection

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Veränderungen der Aktivität einiger Enzyme im Weizenblatt in Abhängigkeit von der temperaturlabilen Verträglichkeit für Puccinia graminis tritici1)

Cited by 44 publications

References 29 publications

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Effect of silicon application on Sorghum bicolor exposed to toxic concentration of zinc

Peroxidase isoenzyme patterns of resistant and susceptible wheat leaves following stem rust infection

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Veränderungen der Aktivität einiger Enzyme im Weizenblatt in Abhängigkeit von der temperaturlabilen Verträglichkeit für Puccinia graminis tritici¹⁾