Vereinfachtes Verfahren zur Gewinnung von humanem Albumin und γ‐Globulin aus Blutplasma mittels Alkoholfällung

The Plasmin Inhibitors of Human Plasma

“…In fact its solubility serves as a criterion for the ade quate performance of the described instructions. Table II summarizes yields and activities of this fraction in com parison with plasma, Cohn's plasma fractions IV, IV4,IV4 and plasma fraction IV prepared by the method of Nitschmann [5]. From the table it can be seen that the "slow" inhibitor was purified 5.9 folds with a recovery of 73.8% whereas the "immediate" inhibitor was purified 3.6 folds with a recovery of 45% (averages obtained from 36 experiments).…”

“…From the table it can be seen that the "slow" inhibitor was purified 5.9 folds with a recovery of 73.8% whereas the "immediate" inhibitor was purified 3.6 folds with a recovery of 45% (averages obtained from 36 experiments). [5], using 95% ethanol.…”

A Simplified Method for the Preparation of Immune-Serum Globulin

“…Although the method described here em ploys the same five variables: ethanol, pro tein concentrations, ionic strength, pH and temperature, as those described previously by others [Cohn et al, 1946;Deutsch et al, 1946;Oncley et al, 1949;Nitschmann et al, 1954;Kistler and Nitschmann, 1962], it dif fers greatly from the classical methods by the immediate separation of the most solu ble protein fraction containing albumin and cq-acid glycoprotein from the rest of the pro teins in the first step. The resulting 42% P precipitate has proved to be an equally ade quate starting material for the recovery of ISG, but its composition is cruder when compared with Cohn fraction II + III or precipitate A by Kistler and Nitschmann's procedure.…”

“…Although the original proce dure has been modified [Deutsch et al, 1946;Nitschmann et al, 1954;Kistler and Nitschmann, 1962;Krijnen et al, 1970; 1 Contribution No. 479 from the American Red Cross Blood Services Laboratories, Bethesda, Md.…”

Side Products of Routine Plasma Fractionation

“…The method of Haupt et al [12] is derived from a frac tionation scheme essentially based on am monium sulfate/Rivanol precipitations. Our starting material is more extensively used as the fractionation scheme of Nitschmann et al [21] and its variations are in current use in many fractionation departments. As com pared to the technique of Haupt et al [12], our method is simpler as it requires neither gel filtration nor preparative electrophoresis.…”