Versatility of Fungal and Bacterial Isolates for Biological Control of Damping-Off Disease Caused byRhizoctonia solaniandPythiumspp.

Harris, A.R.; Adkins, Paul G.

doi:10.1006/bcon.1999.0694

Cited by 35 publications

(9 citation statements)

References 16 publications

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“…Furthermore, the use of endophytic Bacillus strains as biological control candidates has the advantage that they form endospores that can easily be formulated and stored (Ednar et al , 2002). Because of these advantages, the application of endophytic Bacillus strains for biological control of plant diseases caused by soilborne pathogens has been explored (Pleban et al , 1995; Asaka & Shoda, 1996; Raupach & Kloepper, 1998; Chen & Wu, 1999; Harris & Adkins, 1999; Szczech & Shoda, 2004; Ongena et al , 2005) and a number of wild‐type B. subtilis strains have been collected (Podile, 1994; Pleban et al , 1995; Wulff et al , 2002; Szczech & Shoda, 2004; Okigbo, 2005; Ongena et al , 2005; Kapley et al , 2007).…”

Section: Introductionmentioning

confidence: 99%

Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

Gai

et al. 2008

FEMS Microbiology Ecology

145

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Forty-five bacterial isolates were collected from surface-sterilized leaves of mulberry (Morus alba L.). By screening their antagonistic activities against Ralstonia solanacearum in vitro, four isolates showed a remarkable inhibitory effect. The evaluation of the antagonistic strains against bacterial wilt of mulberry indicated that the strain Lu144 effectively reduced disease incidence. In the greenhouse, Lu144 displayed effective biological control against bacterial wilt of mulberry when it was applied to sterile or nonsterile soil before the infection by the pathogen. Based on bacteriological properties and 16S rRNA gene sequencing, Lu144 was identified as a strain of Bacillus subtilis. The endophytic population and infection process of Lu144 in mulberry seedlings was explored following recovery of the green fluorescent protein (GFP)-labeled Lu144 and examination of the labeled strain by confocal laser scanning microscopy. Interestingly, the infection of GFP-labeled Lu144 cells into the mulberry seedlings occurred through the cracks formed at the lateral root junctions and the zone of differentiation and elongation, and the cells were able to develop and transfer in mulberry and mainly in the intercellular spaces of different tissues. The population of the GFP-labeled Lu144 inoculant was larger and more stable in leaves than that in roots and stems.

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Section: Introductionmentioning

confidence: 99%

Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

Gai

et al. 2008

FEMS Microbiology Ecology

145

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“…Among the alternatives, biological control through the use of natural antagonistic microorganisms has been extensively studied and some yeast, fungal and bacterial strains have been shown to be effective against various post-harvest pathogens (Wisniewski and Wilson 1992;Pusey et al 1993). Several strains belonging to the genus Bacillus and particularly to B. subtilis and the closely related B. amyloliquefaciens species were reported effective for the biocontrol of multiple plant diseases caused by soilborne (Asaka and Shoda 1996;Chen and Wu 1999;Harris and Adkins 1999) or post-harvest pathogens (Ferreira et al 1991;Sholberg et al 1995;Mari et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple

et al. 2004

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Aim: Test of Bacillus subtilis strain GA1 for its potential to control grey mould disease of apple caused by Botrytis cinerea. Methods and Results: GA1 was first tested for its ability to antagonize in vitro the growth of a wide variety of plant pathogenic fungi responsible for diseases of economical importance. The potential of strain GA1 to reduce post-harvest infection caused by B. cinerea was tested on apples by treating artificially wounded fruits with endospore suspensions. Strain GA1 was very effective at reducing disease incidence during the first 5 days following pathogen inoculation and a 80% protection level was maintained over the next 10 days. Treatment of fruits with an extract of GA1 culture supernatant also exerted a strong preventive effect on the development of grey mould. Further analysis of this extract revealed that strain GA1 produces a wide variety of antifungal lipopeptide isomers from the iturin, fengycin and surfactin families. A strong evidence for the involvement of such compounds in disease reduction arose from the recovery of fengycins from protected fruit sites colonized by bacterial cells. Conclusions:The results presented here demonstrate that, despite unfavourable pH, B. subtilis endospores inoculated on apple pulp can readily germinate allowing significant cell populations to establish and efficient in vivo synthesis of lipopeptides which could be related to grey mould reduction. Significance and Impact of the Study: This work enables for the first time to correlate the strong protective effect of a particular B. subtilis strain against grey mould with in situ production of fengycins in infected sites of apple fruits.

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“…To overcome the above problems, a non-hazardous alternative such as biological control has been extensively studied, and various microorganisms have been reported in the literature to suppress the phytopathogenic fungi [1,6,15,30,31,48].…”

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confidence: 99%

Production of Biosurfactant Lipopeptides Iturin A, Fengycin and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides

Kim¹,

Ryu²,

Kim³

et al. 2010

Journal of Microbiology and Biotechnology

320

127

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Versatility of Fungal and Bacterial Isolates for Biological Control of Damping-Off Disease Caused byRhizoctonia solaniandPythiumspp.

Cited by 35 publications

References 16 publications

Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple

Production of Biosurfactant Lipopeptides Iturin A, Fengycin and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides

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