Verteilung von [<sup>3</sup>H]Thiopyronin in Hefezellen (Saccharomyces cerevisiae) / Distribution of [<sup>3</sup>H]Thiopyronine in Yeast Cells (Saccharomyces cerevisiae)

The treatment of yeast with thiopyronine (TP) caused a degradation of polyribosomes, even if the cells were not illuminated. In contrast, no differences in the polyribosome profiles of illuminated and unilluminated cells could be seen. Likewise, in in vitro experiments, there was no degradation of polyribosomes caused by dark effect or photodynamic action. Cell-free protein synthesis was inhibited up to 75 per cent by the photodynamic effect when the complete system was treated, but only up to 40 per cent when the polyribosomes or enzyme fraction were treated. Since the enzyme fraction contained aminoacyltRNA-synthetases and tRNA, it was necessary to investigate separately the effect of TP on the enzyme and the tTNA. It was shown that the aminoacyl-tRNA-synthetase and not tRNA was effected by the photodynamic action in its biological activity.

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Verteilung von [³H]Thiopyronin in Hefezellen (Saccharomyces cerevisiae) / Distribution of [³H]Thiopyronine in Yeast Cells (Saccharomyces cerevisiae)

Abstract: After incubation of Saccharomyces cells in [3H] thiopyro nine solution the photodynamically high effective dye was detected only to about 1% in the nucleus of the cell. 99% of the dye is distributed in the cytoplasm.

Cited by 6 publications

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Photodynamic Agents as Tools for Cell Biology

Photodynamic Agents as Tools for Cell Biology

Photodynamischer Effekt bei Hefen. Kalorimetrische Untersuchungen

Photodynamic Action of Thiopyronine on Polyribosomes and Cell-free Protein Synthesis in Yeast

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Verteilung von [3H]Thiopyronin in Hefezellen (Saccharomyces cerevisiae) / Distribution of [3H]Thiopyronine in Yeast Cells (Saccharomyces cerevisiae)

Abstract: After incubation of Saccharomyces cells in [3H] thiopyro­ nine solution the photodynamically high effective dye was detected only to about 1% in the nucleus of the cell. 99% of the dye is distributed in the cytoplasm.

Cited by 6 publications

References 0 publications

Photodynamic Agents as Tools for Cell Biology

Photodynamic Agents as Tools for Cell Biology

Photodynamischer Effekt bei Hefen. Kalorimetrische Untersuchungen

Photodynamic Action of Thiopyronine on Polyribosomes and Cell-free Protein Synthesis in Yeast

Contact Info

Product

Resources

About

Verteilung von [³H]Thiopyronin in Hefezellen (Saccharomyces cerevisiae) / Distribution of [³H]Thiopyronine in Yeast Cells (Saccharomyces cerevisiae)

Abstract: After incubation of Saccharomyces cells in [3H] thiopyro nine solution the photodynamically high effective dye was detected only to about 1% in the nucleus of the cell. 99% of the dye is distributed in the cytoplasm.