Vigna mungo, V. radiata and V. unguiculata plants sampled in different agronomical–ecological–climatic regions of India are nodulated by Bradyrhizobium yuanmingense

Appunu, C.; N’Zoué, Angèle; Moulin, Lionel; Géraldine, Depret; Laguerre, Gisèle

doi:10.1016/j.syapm.2009.05.005

Cited by 39 publications

(27 citation statements)

References 33 publications

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“…and are crossnodulated by Bradyrhizobium spp. and have been shown to share common bradyrhizobial genotypes (Zhang et al 2008;Appunu et al 2009) as seen for IGS profiles I-V in our study. IGS III was predominant in both legumes and was distributed in all the treatments applied to cowpea and green gram.…”

supporting

confidence: 73%

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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Limited information is available on reduced cowpea (Vigna unguiculata L. Walp.) and green gram (Vigna radiata L.Wilczek.) yields in Kenya. Declining soil fertility and absence or presence of ineffective indigenous rhizobia in soils are assumptions that have been formulated but still require to be demonstrated. In this study, soils were collected from legume growing areas of Western (Bungoma), Nyanza (Bondo), Eastern (Isiolo), Central (Meru) and Coast (Kilifi) provinces in Kenya to assess indigenous rhizobia in soils nodulating cowpea and green gram under greenhouse conditions. Our results showed that highest nodule fresh weights of 4.63 and 3.32 g plant À1 for cowpea and green gram were observed in one soil from Isiolo and another from Kilifi, respectively, suggesting the presence of significant infective indigenous strains in both soils. On the other hand, the lowest nodule fresh weights of 2.17 and 0.72 g plant À1 were observed in one soil from Bungoma for cowpea and green gram, respectively. Symbiotic nitrogen (N) fixation by cowpea and green gram was highest in Kilifi soil with values of 98% and 97%, respectively. A second greenhouse experiment was undertaken to evaluate the performance of commercial rhizobial inoculants with both legumes in Chonyi soil (also from Coast province) containing significant indigenous rhizobia [>13.5 Â 10 3 Colony Forming Units (CFU) g À1]. Rhizobial inoculation did not significantly (P < 0.05) affect nodulation, biomass yield and shoot N content in cowpea and green gram compared with controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 16S-23S rDNA intergenic spacer (IGS) region analysis of nodules revealed six groups of which only IGS Group IV corresponded with those from commercial inoculants applied, indicating a lower competitiveness of inoculated strains. In cowpea, IGS III was dominant in nodules of plants inoculated with Biofix and Rizoliq commercial inoculants, and the uninoculated control treatment (63.2, 60 and 52.9%, respectively). Similarly, in green gram, IGS Group III was dominant in nodules of plants inoculated with Biofix 704 and Rizoliq commercial inoculants, and the uninoculated control treatment (75, 73.7 and 61.1%, respectively). Our results suggest that the systematic inoculation of both legumes with current available commercial inoculants to improve biomass yields is not necessary in these regions of Kenya. Also, according to our study, it would make sense to promote the utilization of indigenous strains performing well with both legumes.

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supporting

confidence: 73%

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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“…The ITS and maintenance genes are currently used as markers for molecular systematics and for estimations of phylogenetic relationships among bradyrhizobia [24][25][26][27][28] , with the 16S rRNA gene having few polymorphisms within the Bradyrhizobium genus 25,27,28 [29][30][31] . In the present study, B. yuanmingense strains were isolated from the root nodule of soya bean from Yangon Region and Shan State.…”

Section: Discussionmentioning

confidence: 99%

“…There were some reports on the isolation and classification of B. yuanmingense strains that nodulated mung bean and soya bean 30,31 and B. yuanmingense strain that did not nodulate soya bean but nodulated legume species of the genus Lespedeza 32 . In this study, five B. yuanmingense strains; MAS28 from Yangon Region and MAS13, MAS34, MAS46, and MAS47 from Shan State were isolated.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic diversity of indigenous soya bean bradyrhizobia from different agro-climatic regions in Myanmar

Soe¹,

Yamakawa²,

Hashimoto³

et al. 2013

ScienceAsia

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Soya bean (Glycine max Merr.) is the most important legume crop in the world and an important cash crop in Myanmar. In the present study, we characterized 43 isolates of indigenous soya bean rhizobia from Myanmar based on the sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer region. The sequence analysis confirmed that all isolates were categorized and identified as the genus Bradyrhizobium and they were conspecific with B. japonicum, B. elkanii, B. yuanmingense, and Bradyrhizobium sp. The highest genetic diversity of Bradyrhizobium strains was observed in the Shan State soya bean growing area. B. elkanii was distributed throughout the cultivation areas and was recorded as the dominant soya bean-nodulating strain in Myanmar. The present study revealed that there were two Rj-genotypes as Rj4-and non-Rj-genotypes among the collected soya bean cultivars from Myanmar. These findings indicate a high compatibility between Rj4-genotypes soya beans and nodulation type B strains in Shan State and Yangon region. In Mandalay region, the compatibility between non-Rj-genotype soya beans and nodulation type A strains was clearly investigated. This is the first report describing B. yuanmingense from nodules of soya bean and the geographical distribution of indigenous bradyrhizobia of soya bean from different regions in Myanmar.

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“…According to the results in this study, B. yuanmingense is the most abundant endosymbiont isolated from cowpea nodules. This species represents 99 % of the strains isolated from cowpea in India (Appunu et al 2009), 94 % in Japan (Sarr et al 2009), 40 % in China (Zhang et al 2011), and35 % in Botswana (Steenkamp et al 2008). It is also widely distributed in Asia, Africa, and Latin America (Sarr et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Symbiotic and Agronomic Characterization of Bradyrhizobial Strains Nodulating Cowpea in Northern Peru

Valdez

Soriano

Prado

et al. 2016

Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction

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Eighty bacterial strains were isolated from root nodules of cowpea (Vigna unguiculata) plants agriculturally grown in Northern Peru. After isolation, fifty of them reinfected cowpea and eight showed better symbiotic performance than control plants treated with nitrate both under greenhouse and field. Repetitive extragenic palindromic polymerase chain reaction (REP-PCR) clustered the strains into 3 REP-PCR groups. The partial sequence of the 16S rRNA gene from a representative strain of each REP-PCR pattern showed that the strains were closely

show abstract

Vigna mungo, V. radiata and V. unguiculata plants sampled in different agronomical–ecological–climatic regions of India are nodulated by Bradyrhizobium yuanmingense

Cited by 39 publications

References 33 publications

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Phylogenetic diversity of indigenous soya bean bradyrhizobia from different agro-climatic regions in Myanmar

Symbiotic and Agronomic Characterization of Bradyrhizobial Strains Nodulating Cowpea in Northern Peru

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