Viral Etiology and Clinical Profiles of Children with Severe Acute Respiratory Infections in China

Abstract

BackgroundNo comprehensive analysis is available on the viral etiology and clinical characterization among children with severe acute respiratory infection (SARI) in China during 2009 H1N1 pandemic and post-pandemic period.MethodsCohort of 370 hospitalized children (1 to 72 months) with SARI from May 2008 to March 2010 was enrolled in this study. Nasopharyngeal aspirate (NPA) specimens were tested by a commercial assay for 18 respiratory viral targets. The viral distribution and its association with clinical character were statistically analyzed.ResultsViral pathogen was detected in 350 (94.29%) of children with SARI. Overall, the most popular viruses were: enterovirus/rhinovirus (EV/RV) (54.05%), respiratory syncytial virus (RSV) (51.08%), human bocavirus (BoCA) (33.78%), human parainfluenzaviruse type 3 (PIV3) (15.41%), and adenovirus (ADV) (12.97%). Pandemic H1N1 was the dominant influenza virus (IFV) but was only detected in 20 (5.41%) of children. Moreover, detection rate of RSV and human metapneumovirus (hMPV) among suburb participants were significantly higher than that of urban area (P<0.05). Incidence of VSARI among suburb participants was also significant higher, especially among those of 24 to 59 months group (P<0.05).ConclusionPiconaviruses (EV/RV) and paramyxoviruses are the most popular viral pathogens among children with SARI in this study. RSV and hMPV significantly increase the risk of SARI, especially in children younger than 24 months. Higher incidence of VSARI and more susceptibilities to RSV and hMPV infections were found in suburban patients.

Etiology, seasonality, and clinical characteristics of respiratory viruses in children with respiratory tract infections in Eastern India (Bhubaneswar, Odisha)

“…RSV‐B was detected mainly in the late winter and spring months (9.52%) and in the rainy season (5.6%), in agreement with other studies [Bharaj et al, ; Seo et al, ]. Zhang et al [] reported that hMPV follows a similar seasonality pattern to RSV‐B. In our study, hMPV was detected at the highest rate during the spring (9.52%), PIV‐3 infection was mostly detected during the summer (8.51%) and Inf‐A infection peaked during the autumn season (4.08%).…”

Enteroviruses and Rhinoviruses: Molecular Epidemiology of the Most Influenza-Like Illness Associated Viruses in Senegal

“…In China, Zhang and others (2013) reported 54% of child SARI cases harbored HRV/HEV. 42 However, particularly for RV infections, it is reported that the infection rate is highest during the early years and decreases with age, probably due to the immunity induced by cumulative exposure to different serotypes. 43 However, the presence of a virus in the nasopharynx of a child does not necessarily mean that it is the etiological agent of the ILI; indeed, it may only represent a coincidental upper airway infection, an asymptomatic carrier state, or prolonged shedding of a pathogen that caused a previous infection.…”

Genetic characterization of human bocavirus among children with severe acute respiratory infection in China

“…HBoV co-infection with other respiratory viruses, including HRSV, HRV/EV, HAdV, HMPV, HPIV1e4, influenza A/B virus and HCoVs (-OC43, -229E, -NL63, -HKU1), was also screened as described previously. 20,21 Sequencing and phylogenetic analysis of HBoV1 genome Four samples of HBoV1 infection only in this study were used for complete genomes sequencing by next-generation sequencing. Samples were pretreated as previously, 22 and the amplified DNA was used as a template for Illumina Hiseq 2500 sequencing, paired-end reads (2 Â 125 bp reads) were assembled into contigs by CLC genomic workbench.…”