Viral Load Distribution of Cucumber Green Mottle Mosaic Virus in Leaves

Abstract

Data on the viral load distribution by different leaves parts (upper, middle, lower leaves) in cucumber plants grown in protected ground are presented. Studies were conducted at the time of the visible infection manifestation. It was revealed that accumulation of virions of the cucumber green mottle mosaic virus (CGMMV), with the onset of manifestation, prevailed in the middle and lower leaves, which is probably due to the "slow" redistribution of the virus along the external phloem. This pattern is confirmed by a high correlation coefficient (r = 0,99). It was also found that the viral load indicator has high variability (C v = 63,77%). A similar coefficient of variation may indicate the fact that under constant environmental and nutritional conditions, which are provided under the conditions of protected ground, the individual susceptibility of cucumber plants to the phytopathogen affects the number of viral particles in the plant.

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“…In a similar context, Spiegel et al [27] have described the uneven distribution of the tobacco streak virus (TSV) in the shoot and root systems of infected strawberry plantlets and that up to 30% of the progeny plants tested negative by ELISA. Plotnikov et al [28] have shown that there is a correlation between the viral load of the cucumber green mottle mosaic virus (CGMMV) and the part of the cucumber plant (r = 0.99). Moreover, the same authors have found that high values of CGMMV concentration in the cucumber leaves of greenhouse plants were observed on the middle (46%) and lower (36%) leaves.…”