Background This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes Ia and Ib, which differ from genotype I. Several hypotheses on the origin of the different genotypes in Siberia are discussed. Results We obtained varying length of the restriction fragments for genotypes I. Additionally using restrictase Hae III were received fragments was named genotype Ia, and genotype Ib. There are 2.57 ± 0.55% (20 out of 779) samples of genotype Ib which does not differ significantly from 1% (χ2 = 2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73%). The maximum viral load was observed in animals with the II and IV viral genotypes (1000–1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype Ib (from 700 to 900 viral particles per 1000 healthy cells). Conclusions The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (Ib) was found, which can be both a neoplasm and a relict strain.

Data on the viral load distribution by different leaves parts (upper, middle, lower leaves) in cucumber plants grown in protected ground are presented. Studies were conducted at the time of the visible infection manifestation. It was revealed that accumulation of virions of the cucumber green mottle mosaic virus (CGMMV), with the onset of manifestation, prevailed in the middle and lower leaves, which is probably due to the "slow" redistribution of the virus along the external phloem. This pattern is confirmed by a high correlation coefficient (r = 0,99). It was also found that the viral load indicator has high variability (C v = 63,77%). A similar coefficient of variation may indicate the fact that under constant environmental and nutritional conditions, which are provided under the conditions of protected ground, the individual susceptibility of cucumber plants to the phytopathogen affects the number of viral particles in the plant.

The aim of the study was to assess the possibilities of noninvasive diagnosis of liver fibrosis (FIB-4 and APRI indices) in patients with CHC and abdominal obesity.Materials and methods. 52 men with CHC were examined. Genotype 1 was determined in 24 patients, genotype 3 in 19 patients and genotype 2 in 9 patients. According to the severity of fibrosis, patients with CHC were divided: without fibrosis (F0) - 12 patients, with weak fibrosis (F1) - 17 patients, with moderate fibrosis (F2) - 10 patients, with severe fibrosis (F3) - 8 patients, cirrhosis of the liver (F4) was detected in 5 patients. According to a liver biopsy, steatosis was found in 18 patients with CHC. Abdominal obesity was found in 34 patients with CHC. Non-invasive diagnosis of liver fibrosis was assessed using routine FIB-4 and APRI indices. The interval of values of FIB-4 and APRI, not related to the criteria for assessing the stage of fibrosis F3 and F4, we have conventionally designated as the «gray zone». The presence of insulin resistance was evaluated at HOMA-IR> 2.Results. Key values of the FIB-4 index in patients with CHC and abdominal obesity were found significantly more often than when calculating the APRI index. Insulin resistance in patients with CHC and abdominal obesity was statistically significantly more frequent than in patients with CHC and without abdominal obesity. At stages F3-F4 in patients with CHC, abdominal obesity and insulin resistance, APRI values were recorded more often in the «gray zone»than FIB-4 values.Conclusion. The FIB-4, APRI, HOMA-IR indices can be used in patients with CHC and abdominal obesity during the follow-up and dynamic monitoring of patients with CHC to highlight risk groups. FIB-4 was significantly more informative for determining the stage of fibrosis than APRI in patients with CHC and abdominal obesity with insulin resistance (HOMA-IR> 2).

The article deals with some issues of geometric modeling of ship hull structures in specialized CAD system. Stiffened shells and platings should be idealized as a set of elementary plate panels for the purpose of structural design using local strength and buckling requirements. In the process of geometric modeling and creating the database for calculation, a special searching algorithm for closed loops of every panel should be used. This algorithm is to have good performance and versatility. In this paper, the authors suggest an original algorithm used in CADS-Hull software developed in SMTU. It is based on a regular field of points generation within the large contour of the considered structure. A series of rays is built from every point to find intersections. It is shown that this algorithm is quite good for structures (expansions, decks, bulkheads, etc.) with non-orthogonal boundaries. Some tasks for logical operations with found panels are also discussed. One of them is the clipping of a panel or plate polygon by boundaries of a considered structure (expansion contour, hull lines). The authors developed a generic method of polygons clipping. It is based on a rotation of clipping convex polygons together with the clipped polygons. All faces of the latter that are in the negative half-plane are removed. Some problems of collecting data for every found panel are discussed. An original algorithm of smaller and larger size definition for irregular and triangular panels is also given in this paper.

During the study, the phaseolin types in 24 bean varieties from the Novosibirsk SAU collection were determined: 20 varieties are of T-type, 2 varieties - S-type. Two varieties showed a type of phaseolin previously undetermined by polymerase chain reaction (PCR) method. It was conditionally designated as N-type by us. Statistical analysis of the relationship of phaseolin type with economic and valuable traits showed a reliable relationship with the mass of 1000 seeds and the number of seeds from the plant. A number of other traits showed evidence of differences uncertainty between phaseolin types. Further research with a large number of varieties is therefore required. In the early stages of the breeding process, however, the phaseolin type can be used in the selection of forms with valuable breeding traits.