Background This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes Ia and Ib, which differ from genotype I. Several hypotheses on the origin of the different genotypes in Siberia are discussed. Results We obtained varying length of the restriction fragments for genotypes I. Additionally using restrictase Hae III were received fragments was named genotype Ia, and genotype Ib. There are 2.57 ± 0.55% (20 out of 779) samples of genotype Ib which does not differ significantly from 1% (χ2 = 2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73%). The maximum viral load was observed in animals with the II and IV viral genotypes (1000–1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype Ib (from 700 to 900 viral particles per 1000 healthy cells). Conclusions The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (Ib) was found, which can be both a neoplasm and a relict strain.

Data on the viral load distribution by different leaves parts (upper, middle, lower leaves) in cucumber plants grown in protected ground are presented. Studies were conducted at the time of the visible infection manifestation. It was revealed that accumulation of virions of the cucumber green mottle mosaic virus (CGMMV), with the onset of manifestation, prevailed in the middle and lower leaves, which is probably due to the "slow" redistribution of the virus along the external phloem. This pattern is confirmed by a high correlation coefficient (r = 0,99). It was also found that the viral load indicator has high variability (C v = 63,77%). A similar coefficient of variation may indicate the fact that under constant environmental and nutritional conditions, which are provided under the conditions of protected ground, the individual susceptibility of cucumber plants to the phytopathogen affects the number of viral particles in the plant.

This publication presents the results of the analysis of the dynamics of changes in the largefruited indicators of the breeding group of minipigs of the ICG SB RAS. The analysis showed that the four large-copious indicators are divided into two pairs. The first pair is made up of sample values of the characteristic: average and maximum. These indicators are characterized by stability throughout the studied period. The second pair includes the sample minimum values and standard deviations of the trait. These two indicators are dynamic: the sample minimum values are characterized by a decrease, and the sample standard deviations are characterized by a uniform increase, described by linear regression equations. It is shown that the dynamic characteristics are related to each other. It is determined that in this complex, the leader is the minimum value, and the follower is the standard deviation. This is explained by the fact that an increase in the standard deviation is associated with a decrease in the minimum value and the stability of the maximum in the studied period of time. The result of this process is the growth of the genetic potential in the breeding group, which is responsible for the high weight of the newborn individual. However, due to the small size of sows in comparison with commercial breeds (60-70 kg), this potential cannot be realized. Nevertheless, its redundancy ensures the stabilization of the maximum and average values of the trait - the mass of a newborn individual in minipigs of the ICG SB RAS. A possible way to increase the realization of the potential of large-copious breeding group is to reduce the multiple fertility of sows, which is quite solvable, but hardly advisable. Thus there is natural selection directed against individuals with a low birth weight in the herd. Natural and artificial selection for live weight of piglets at birth of 700 g or more, both help to stabilize the average value of the trait at the level optimal for the broodstock.

This study is the biodiversity and properties of bovine leukemia virus in Western Siberia. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes Ia and Ib, which differ from genotype I. Restrictase Hae III sections the nucleotide sequence of the env gene intofragments with lengths of 316-27-95-5 bp (genotype I), 31-285-27-95-5 bp (genotype Ia), and 31-85-200-27-100 bp (genotype Ib). There are 2.57±0.55% (20 out of 779) samples of genotype Ib which does not differ significantly from 1% (χ2=2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73 %). This paper explores the effect of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The maximum viral load was observed in animals with the II and IV viral genotypes (1000 – 1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype Ib (from 700 to 900 viral particles per 1000 healthy cells). Several hypotheses on the origin of the different genotypes in Siberia are discussed. The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (Ib) was found, which can be both a neoplasm and a relict strain.

This study is the biodiversity and properties of bovine leukemia virus in Western Siberia. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes Ia and Ib, which differ from genotype I. Restrictase Hae III sections the nucleotide sequence of the env gene intofragments with lengths of 316-27-95-5 bp (genotype I), 31-285-27-95-5 bp (genotype Ia), and 31-85-200-27-100 bp (genotype Ib). There are 2.57±0.55% (20 out of 779) samples of genotype Ib which does not differ significantly from 1% (χ2=2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73 %). This paper explores the effect of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The maximum viral load was observed in animals with the II and IV viral genotypes (1000 – 1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype Ib (from 700 to 900 viral particles per 1000 healthy cells). Several hypotheses on the origin of the different genotypes in Siberia are discussed. The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (Ib) was found, which can be both a neoplasm and a relict strain.