Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent

Campos, Rafael K.; Andrade, Kétyllen Reis; Ferreira, Paulo César Peregrino; Bonjardim, Cláudio Antônio; Scola, Bernard La; Kroon, Erna Geessien; Abrahão, Jônatas Santos

doi:10.1016/j.jcv.2012.08.009

Cited by 20 publications

(13 citation statements)

References 19 publications

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“…For future work, we believe that the combination of liquid culture of amoebae in microplates with one or more blind enrichment steps and the detection of amoebal lysis by an automated flow cytometer will be the best strategy for optimal high-throughput analysis. Conversely, the use of ethanol to decontaminate samples without killing viruses, especially mimiviruses, should be a suitable alternative to the use of antibiotics in situations where cultures need to be performed on non-axenic protozoa [22,23]. A unique proposed alternative was published in a report related to the isolation of Megavirus chilensis [24].…”

Section: Discussionmentioning

confidence: 99%

A Decade of Improvements in Mimiviridae and Marseilleviridae Isolation from Amoeba

et al. 2013

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Since the isolation of the first giant virus, the Mimivirus, by T.J. Rowbotham in a cooling tower in Bradford, UK, and after its characterisation by our group in 2003, we have continued to develop novel strategies to isolate additional strains. By first focusing on cooling towers using our original time-consuming procedure, we were able to isolate a new lineage of giant virus called Marseillevirus and a new Mimivirus strain called Mamavirus. In the following years, we have accumulated the world's largest unique collection of giant viruses by improving the use of antibiotic combinations to avoid bacterial contamination of amoeba, developing strategies of preliminary screening of samples by molecular methods, and using a high-throughput isolation method developed by our group. Based on the inoculation of nearly 7,000 samples, our collection currently contains 43 strains of Mimiviridae (14 in lineage A, 6 in lineage B, and 23 in lineage C) and 17 strains of Marseilleviridae isolated from various environments, including 3 of human origin. This study details the procedures used to build this collection and paves the way for the high-throughput isolation of new isolates to improve the record of giant virus distribution in the environment and the determination of their pangenome.

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Section: Discussionmentioning

confidence: 99%

A Decade of Improvements in Mimiviridae and Marseilleviridae Isolation from Amoeba

et al. 2013

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“…This technician habitually made an annual check of his serologies to the microbes he manipulated. Mimivirus is known for its extreme resistance to degradation by physical and chemical agents [70], and amoebae can also protect them from degradation [71,72]. The technician came for medical consultation with one of us (D.R.)…”

Section: Evidence Of Human Infection or Colonization With A Giant Virusmentioning

confidence: 99%

Giant Viruses of Amoebae as Potential Human Pathogens

2013

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Giant viruses infecting phagocytic protists are composed of mimiviruses, the record holders of particle and genome size amongst viruses, and marseilleviruses. Since the discovery in 2003 at our laboratory of the first of these giant viruses, the Mimivirus, a growing body of data has revealed that they are common inhabitants of our biosphere. Moreover, from the outset, the story of Mimivirus has been linked to that of patients exhibiting pneumonia and it was shown that patients developed antibodies to this amoebal pathogen. Since then, there have been several proven cases of human infection or colonization with giant viruses of amoebae, which are known to host several bacteria that are human pathogens. Mimiviruses and marseilleviruses represent a major challenge in human pathology, as virological procedures implemented to date have not used appropriate media to allow their culture, and molecular techniques have used filtration steps that likely prevented their detection. Nevertheless, there is an increasing body of evidence that mimiviruses might cause pneumonia and that humans carry marseilleviruses, and re-analyses of metagenomic databases have provided evidence that these giant viruses can be common in human samples. The proportion of human infections related to these giant mimiviruses and marseilleviruses and the precise short- and long-term consequences of these infections have been scarcely investigated so far and should be the subject of future works.

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“…Considering the hypothetical role of APMV as pneumonia agent associated with prolonged mechanical ventilation the viral stability in ventilator devices was evaluated. Three different brands of sterile ventilator device (tube) (VD) were used to test APMV recovery (five quadrants of 2×2 cm per brand) [21]. In this case, 10 µl of viral suspension was added to VD quadrants with or without BAV, which were maintained in sterile Petri dishes.…”

Section: Methodsmentioning

confidence: 99%

Acanthamoeba polyphaga mimivirus Stability in Environmental and Clinical Substrates: Implications for Virus Detection and Isolation

et al. 2014

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Viruses are extremely diverse and abundant and are present in countless environments. Giant viruses of the Megavirales order have emerged as a fascinating research topic for virologists around the world. As evidence of their ubiquity and ecological impact, mimiviruses have been found in multiple environmental samples. However, isolation of these viruses from environmental samples is inefficient, mainly due to methodological limitations and lack of information regarding the interactions between viruses and substrates. In this work, we demonstrate the long-lasting stability of mimivirus in environmental (freshwater and saline water) and hospital (ventilator plastic device tube) substrates, showing the detection of infectious particles after more than 9 months. In addition, an enrichment protocol was implemented that remarkably increased mimivirus detection from all tested substrates, including field tests. Moreover, biological, morphological and genetic tests revealed that the enrichment protocol maintained mimivirus particle integrity. In conclusion, our work demonstrated the stability of APMV in samples of environmental and health interest and proposed a reliable and easy protocol to improve giant virus isolation. The data presented here can guide future giant virus detection and isolation studies.

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Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent

Abstract: The data presented herein may help health and laboratory workers plan the best strategy to control this putative pneumonia agent from surfaces and devices.

Cited by 20 publications

References 19 publications

A Decade of Improvements in Mimiviridae and Marseilleviridae Isolation from Amoeba

A Decade of Improvements in Mimiviridae and Marseilleviridae Isolation from Amoeba

Giant Viruses of Amoebae as Potential Human Pathogens

Acanthamoeba polyphaga mimivirus Stability in Environmental and Clinical Substrates: Implications for Virus Detection and Isolation

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