Virus adsorption in a complex system: an experimentally designed study

Quignon, F.; Thomas, F.; Gantzer, Christophe; Huyard, A.; Schwartzbrod, L.

doi:10.1016/s0043-1354(97)00331-x

Cited by 18 publications

(7 citation statements)

References 21 publications

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“…In this case, hydrophobic interactions would be the main force to overcome in desorbing phages from soil surfaces. Beef extract consists of large organic molecules that most likely disrupt hydrophobic interactions between phages and soil surfaces (22,37; Y. Jin, personal communication), whereas glycine is a low-molecular-weight amphipathic amino acid and may be able to solvate viruses and prevent hydrophobic interactions between phages and soil particles (8,16,22). For these reasons, glycine and beef extract may be more effective than potassium citrate or sodium pyrophosphate in displacing adsorbed phages from binding sites.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have detailed the mechanics and kinetics of virus adsorption to soil particles (16,22,29,31), as well as the physical and chemical factors influencing adsorption and desorption, including solution pH, the isoelectric point of phage capsids (18), ionic strength and composition of the eluant (37,49), and hydrophobic interactions (22; for a review, see reference 19). Obviously, several concerted factors dictate the phage-soil interactions.…”

mentioning

confidence: 99%

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Sampling Natural Viral Communities from Soil for Culture-Independent Analyses

Williamson

Wommack

Radosevich

2003

Appl Environ Microbiol

156

122

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An essential first step in investigations of viruses in soil is the evaluation of viral recovery methods suitable for subsequent culture-independent analyses. In this study, four elution buffers (10% beef extract, 250 mM glycine buffer, 10 mM sodium pyrophosphate, and 1% potassium citrate) and three enumeration techniques (plaque assay, epifluorescence microscopy [EFM], and transmission electron microscopy [TEM]) were compared to determine the best method of extracting autochthonous bacteriophages from two Delaware agricultural soils. Beef extract and glycine buffer were the most effective in eluting viable phages inoculated into soils (up to 29% recovery); however, extraction efficiency varied significantly with phage strain. Potassium citrate eluted the highest numbers of virus-like particles from both soils based on enumerations by EFM (mean, 5.3 ؋ 10 8 g of dry soil ؊1 ), but specific soil-eluant combinations posed significant problems to enumeration by EFM. Observations of virus-like particles under TEM gave confidence that the particles were, in fact, phages, but TEM enumerations yielded measurements of phage abundance (mean, 1.5؋10 8 g of dry soil ؊1 ) that were about five times lower. Clearly, the measurement of phage abundance in soils varies with both the extraction and enumeration methodology; thus, it is important to assess multiple extraction and enumeration approaches prior to undertaking ecological studies of phages in a particular soil.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Sampling Natural Viral Communities from Soil for Culture-Independent Analyses

Williamson

Wommack

Radosevich

2003

Appl Environ Microbiol

156

122

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“…Adsorption is one of the processes, which besides being widely used for dye removal also has wide applicability in wastewater treatment (Bansal and Goyal, 2005;Danis et al, 1998;Freeman, 1989;Imamura et al, 2002;Liapis, 1987;Mantell, 1951;Mattson and Mark, 1971;Pirbazari et al, 1991;Quignon et al, 1998;Weber Jr. et al, 1970). The term adsorption refers to a process wherein a material is concentrated at a solid surface from its liquid or gaseous surroundings.…”

Section: Adsorption and Ion Exchangementioning

confidence: 99%

Application of low-cost adsorbents for dye removal – A review

Gupta

Suhas

2009

Journal of Environmental Management

3,258

1,353

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“…This characteristic of urea was thought to contribute to the efficient extraction of crude protein and isolation of VBPs from bacterial cultures derived from activated sludge. The mechanism of adsorbing VBPs to the viral peptide in the affinity column cannot be explained by the simple electrostatic attraction and hydrophobic effect, although they have been reported as main factors in the adsorption of viruses to several substances, such as clay, sandy soil, estuarine sediments, and so on (5,6,21,22,26,39). Under the condition of the start buffer (pH 8.0), the net charge of the viral peptide in the affinity column was expected to be negative, because the pI of the immobilized viral peptide was estimated to be 3.88 (34).…”

Section: Vol 70 2004 Virus-binding Proteins Recovered From Sludge Cmentioning

confidence: 99%

Virus-Binding Proteins Recovered from Bacterial Culture Derived from Activated Sludge by Affinity Chromatography Assay Using a Viral Capsid Peptide

Sano

Matsuo

Omura

2004

Appl Environ Microbiol

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The contamination of water environments by pathogenic viruses has raised concerns about outbreaks of viral infectious diseases in our society. Because conventional water and wastewater treatment systems are not effective enough to inactivate or remove pathogenic viruses, a new technology for virus removal needs to be developed. In this study, the virus-binding proteins (VBPs) in a bacterial culture derived from activated sludge were successfully recovered. The recovery of VBPs was achieved by applying extracted crude proteins from a bacterial culture to an affinity column in which a custom-made peptide of capsid protein from the poliovirus type 1 (PV1) Mahoney strain (H 2 N-DNPASTTNKDKL-COOH) was immobilized as a ligand. VBPs exhibited the ability to adsorb infectious particles of PV1 Sabin 1 as determined by enzyme-linked immunosorbent assay. The evaluation of surface charges of VBPs with ion-exchange chromatography found that a majority of VBP molecules had a net negative charge under the conditions of affinity chromatography. On the other hand, a calculated isoelectric point implied that the viral peptide in the affinity column was also charged negatively. As a result, the adsorption of the VBPs to the viral peptide in the affinity column occurred with a strong attractive force that was able to overcome the electrostatic repulsive force. Two-dimensional electrophoresis revealed that the isolated VBPs include a number of proteins, and their molecular masses were widely distributed but smaller than 100 kDa. Amino acid sequences of N termini of five VBPs were determined. Homology searches for the N termini against all protein sequences in the National Center for Biotechnology Information (NCBI) database showed that the isolated VBPs in this study were newly discovered proteins. These VBPs that originated with bacteria in activated sludge might be stable, because they are existing in the environment of wastewater treatments. Therefore, a virus removal technology utilizing VBPs as viral adsorbents can be developed, since it is possible to replicate VBPs by protein cloning techniques.

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Virus adsorption in a complex system: an experimentally designed study

Cited by 18 publications

References 21 publications

Sampling Natural Viral Communities from Soil for Culture-Independent Analyses

Sampling Natural Viral Communities from Soil for Culture-Independent Analyses

Application of low-cost adsorbents for dye removal – A review

Virus-Binding Proteins Recovered from Bacterial Culture Derived from Activated Sludge by Affinity Chromatography Assay Using a Viral Capsid Peptide

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