Virus Content as an Index of Symptomatic Resistance to Barley Yellow Dwarf Virus in Cereals

Skaria, M.; Lister, R. M.; Foster, John E.; Shaner, Gregory

doi:10.1094/phyto-75-212

Cited by 44 publications

(14 citation statements)

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“…Sip et al (2006) found a positive correlation between ELISA values estimated in barley seedlings of the cross 'Igri' (ryd2) £ 'Atlas 68' (Ryd2) and the symptom level observed in Weld experiments, indicating that Ryd2 decreases viral reproduction in the plant at least in the seedling stage, and hence is a gene for resistance rather than tolerance. Furthermore, Larkin et al (1991) and Ranieri et al (1993) found a positive correlation between symptom expression and ELISA values, which was also more pronounced in younger plants, whereas Scheurer et al (2000) and Skaria et al (1985) found no relation between the virus titre and the level of tolerance. However, in these experiments the virus titre was measured in older plants.…”

Section: Discussionmentioning

confidence: 84%

“…Ryd2 has been reported to reduce the virus titre of BYDV-PAV and -MAV in young plants (Skaria et al 1985;Baltenberger et al 1987;Herrera and Plumb 1991;Ranieri et al 1993;Chalhoub et al 1994Chalhoub et al , 1995Sip et al 2006), but only to some isolates of CYDV-RPV (Banks et al 1992). However, no diVerences in the virus titre of plants carrying Ryd2 or ryd2, respectively concerning BYDV-PAV were observed in studies in which older plants were tested (Skaria et al 1985;Scheurer et al 2000). Besides this, diVerent alleles may be present at this locus (Catherall et al 1970;Chalhoub et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

et al. 2011

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Barley yellow dwarf virus (BYDV) is an economically important pathogen of barley, which may become even more important due to global warming. In barley, several loci conferring tolerance to BYDV-PAV-ASL-1 are known, e.g. Ryd2, Ryd3 and a quantitative trait locus (QTL) on chromosome 2H. The aim of the present study was to get information whether the level of tolerance against this isolate of BYDV in barley can be improved by combining these loci. Therefore, a winter and a spring barley population of doubled haploid (DH) lines were genotyped by molecular markers for the presence of the susceptibility or the resistance encoding allele at respective loci (Ryd2, Ryd3, QTL on chromosome 2H) and were tested for their level of BYDV-tolerance after inoculation with viruliferous (BYDV-PAV-ASL-1) aphids in Weld trials. In DH-lines carrying the combination Ryd2 and Ryd3, a signiWcant reduction of the virus titre was detected as compared to lines carrying only one of these genes. Furthermore, spring barley DH-lines with this allele combination also showed a signiWcantly higher relative grain yield as compared to lines carrying only Ryd2 or Ryd3. The QTL on chromosome 2H had only a small eVect on the level of tolerance in those lines carrying only Ryd2, or Ryd3 or a combination of both, but the eVect in comparison to lines carrying no tolerance allele was signiWcant. Overall, these results show that the combination of Ryd2 and Ryd3 leads to quantitative resistance against BYDV-PAV instead of tolerance.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

et al. 2011

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“…Resistance in oats to BYDV appears to correlate with virus concentration (Hammond et al 1983, Skaria et al 1985, and breeders have used virus concentration as a selection criterium to improve the resistance of oat lines to BYDV. In this study, virus concentration was monitored by ELISA and using antiserum against an RPV isolate.…”

Section: Discussionmentioning

confidence: 99%

Yield reduction of oat cultivars in relation to disease development caused by barley yellow dwarf virus

Peltonen-Sainio

Karjalainen

1990

AFSci

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Abstract. The effects of barley yellow dwarf virus on the development, grain yield and yield components of oat cultivar Veli and breeding lines Hankkija 78152 and 78033 were studied in two years. Cultivar differences to BYDV infection were best observed at a low infection level. Single tilleranalysis indicated close correlation between symptom severity and reduction in main yield components. Early infection caused greatest yield losses in all cultivars. BYDV strongly reduced both plant height and harvest index. Mild infection reduced the grain yield by 2-B %, but severe infection by 36-41°/o. The number of grains and panicle weight were strongly reduced, but the 1000-grain weight was only slightly affected after severe BYDVinfection. Implications of these results for disease control are discussed.

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“…The results presented here further confirmed the ease of following the development of WSM symptoms in wheat-Thinopyrum amphiploids as well as their derivatives. Studies have demonstrated that symptom development was associated with a build up of the virus, in terms of ELISA color intensity as a measure of the serological quantification of viral protein (Jedlinski et al 1977;Skaria et al 1985;Ranieri et al 1993). However, a similar association could not be clearly drawn in a study involving wild wheat species (r 2 ¼ 0.262) (Stoddard et al 1987b).…”

Section: Discussionmentioning

confidence: 99%

Promising genetic resources for resistance to wheat streak mosaic virus and the wheat curl mite in wheat-Thinopyrum partial amphiploids and their derivatives

Conner

Chen

et al. 2005

Genet Resour Crop Evol

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Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite (WCM), Aceria tosichella Keifer, is one of the most destructive viral diseases of wheat found in many wheat producing areas of the world. Host resistance is the most effective method for controlling this disease and its vector. Symptomatological analysis and enzymelinked immunosorbent assay (ELISA) were used to characterize WSMV-resistance in wheat-alien partial amphiploid lines and their derivatives. The results showed that most of partial amphiploids derived from Thinopyrum ponticum and Th. intermedium were free of systemic symptoms with very low ELISA readings that were similar to that of the non-inoculated Chinese Spring control. While the partial amphiploid lines 693 and PWM706 were identified as new genetic resources of resistance to WSMV. The present study demonstrated that both symptomatological and ELISA methods efficiently assessed WSMV-resistance in the wheat-alien hybrids and systemic symptom incidence and ELISA absorbance readings were highly correlated (r 2 ¼ 0.8658-0.9323) over time following inoculation. The ELISA results also indicated that the virus did not buildup in the plant tissues of these virus-resistant partial amphiploids. Similar results were observed in chromosome translocation and substitution lines that have the gene Wsm1 conferring WSMV resistance. However, the lines containing the gene Wsm1 and all the partial amphiploid lines, except Agrotana, were susceptible to the WCM. One line derived from a cross of wheat and Agrotana, was effective in controlling the spread of WSMV and was highly resistant to the WCM. Another line and an accession of Triticum dicoccoides (Koern.) Schweinf. were highly susceptible to WSMV and WCM. Early disease development was delayed in a new hard red winter cultivar McClintock. The partial WSMV-resistance of McClintock was demonstrated by initially low ELISA readings, and a lower percentage of infected plants than other WSMV-susceptible wheat. The use of the identified promising sources of resistance to WSMV and the WCM in wheat breeding is discussed.

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Virus Content as an Index of Symptomatic Resistance to Barley Yellow Dwarf Virus in Cereals

Cited by 44 publications

References 0 publications

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

Yield reduction of oat cultivars in relation to disease development caused by barley yellow dwarf virus

Promising genetic resources for resistance to wheat streak mosaic virus and the wheat curl mite in wheat-Thinopyrum partial amphiploids and their derivatives

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