Visiting the cell biology of Salmonella infection

Lahiri, Amit; Lahiri, Ayan; Iyer, Namrata; Das, Priyanka; Chakravortty, Dipshikha

doi:10.1016/j.micinf.2010.05.010

Cited by 54 publications

(54 citation statements)

References 66 publications

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“…Stationary-phase cultures (S. Typhimurium and S. Typhi) were diluted 1:33 in LB and grown for 3 h (late exponential phase) for infection of epithelial cells (intestine 407). This was done to induce the SPI-1 genes required for invasion of nonphagocytic cells (29). The gentamicin protection assay was performed as described previously (48).…”

Section: Methodsmentioning

confidence: 99%

“…Salmonella enterica is a Gram-negative, intracellular, non-spore-forming bacillus of the Enterobacteriaceae family and causes a wide array of symptoms, such as gastroenteritis, bacteremia, and typhoid fever (28). These bacteria translocate across the epithelial cells via a Salmonella pathogenicity island 1 (SPI 1)-dependent mechanism, after which they infect macrophages and, prior to dissemination, remain inside vacuoles (29,30). Among the numerous drugs in vogue, fluoroquinolones, such as ciprofloxacin, and broad-spectrum cephalosporins, such as ceftriaxone and ceftiofur, are the drugs of choice to combat typhoid fever.…”

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confidence: 99%

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Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Gnanadhas

Thomas

et al. 2013

Antimicrob Agents Chemother

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150

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dThe emergence of multidrug-resistant bacteria is a global threat for human society. There exist recorded data that silver was used as an antimicrobial agent by the ancient Greeks and Romans during the 8th century. Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities, with minimal cytotoxic effects on the cells. However, very few reports have shown the usage of AgNPs for antibacterial therapy in vivo. In this study, we deciphered the importance of the chosen methods for synthesis and capping of AgNPs for their improved activity in vivo. The interaction of AgNPs with serum albumin has a significant effect on their antibacterial activity. It was observed that uncapped AgNPs exhibited no antibacterial activity in the presence of serum proteins, due to the interaction with bovine serum albumin (BSA), which was confirmed by UV-Vis spectroscopy. However, capped AgNPs [with citrate or poly(vinylpyrrolidone)] exhibited antibacterial properties due to minimized interactions with serum proteins. The damage in the bacterial membrane was assessed by flow cytometry, which also showed that only capped AgNPs exhibited antibacterial properties, even in the presence of BSA. In order to understand the in vivo relevance of the antibacterial activities of different AgNPs, a murine salmonellosis model was used. It was conclusively proved that AgNPs capped with citrate or PVP exhibited significant antibacterial activities in vivo against Salmonella infection compared to uncapped AgNPs. These results clearly demonstrate the importance of capping agents and the synthesis method for AgNPs in their use as antimicrobial agents for therapeutic purposes.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Gnanadhas

Thomas

et al. 2013

Antimicrob Agents Chemother

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150

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“…In general, the ability of Salmonella to cause an infection in humans or animals depends on the innate ability of the bacteria to encode and express a set (or combination) of virulence genes that can evade and neutralize host defenses. These factors are associated with pathogenic-ity islands, virulence plasmids, toxins, fimbriae, and flagella (28,40,(67)(68)(69).…”

Section: Survival In Macrophages and Dendritic Cellsmentioning

confidence: 99%

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Foley

Johnson

Ricke

et al. 2013

Microbiol Mol Biol Rev

270

218

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SUMMARY Enteric pathogens such as Salmonella enterica cause significant morbidity and mortality. S. enterica serovars are a diverse group of pathogens that have evolved to survive in a wide range of environments and across multiple hosts. S. enterica serovars such as S . Typhi, S . Dublin, and S . Gallinarum have a restricted host range, in which they are typically associated with one or a few host species, while S . Enteritidis and S . Typhimurium have broad host ranges. This review examines how S. enterica has evolved through adaptation to different host environments, especially as related to the chicken host, and continues to be an important human pathogen. Several factors impact host range, and these include the acquisition of genes via horizontal gene transfer with plasmids, transposons, and phages, which can potentially expand host range, and the loss of genes or their function, which would reduce the range of hosts that the organism can infect. S . Gallinarum, with a limited host range, has a large number of pseudogenes in its genome compared to broader-host-range serovars. S. enterica serovars such as S . Kentucky and S . Heidelberg also often have plasmids that may help them colonize poultry more efficiently. The ability to colonize different hosts also involves interactions with the host's immune system and commensal organisms that are present. Thus, the factors that impact the ability of Salmonella to colonize a particular host species, such as chickens, are complex and multifactorial, involving the host, the pathogen, and extrinsic pressures. It is the interplay of these factors which leads to the differences in host ranges that we observe today.

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“…While Salmonella is largely killed through and NADPH oxidase-dependent mechanism by generating excess H 2 O 2 in the phagosomes of neutrophils and monocytes, most live Salmonella organisms can reside in the phagosomes of macrophages, with apparently little impact of NADPH oxidase on the generation of H 2 O 2 (28). In addition to the disparate effects of host cells on ROS production, bacterial resistance to oxidative stress allows intracellular bacteria to grow and survive in host cells (29)(30)(31)). The present study shows that the overexpression of MdsABC leads to a small change in the phenotype of Salmonella by increasing secretion of PSPS but confers some resistance to ROS-producing phagocytosis.…”

Section: Discussionmentioning

confidence: 99%

MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium

et al. 2015

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e MdsABC is a Salmonella-specific tripartite efflux pump that has been implicated in the virulence of Salmonella enterica serovar Typhimurium; however, little is known about the virulence factors associated with this pump. We observed MdsABC expression-dependent alterations in the degree of resistance to extracellular oxidative stress and macrophage-mediated killing. Thinlayer chromatography and tandem mass spectrometry analyses revealed that overexpression of MdsABC led to increased secretion of 1-palmitoyl-2-stearoyl-phosphatidylserine (PSPS), affecting the ability of the bacteria to invade and survive in host cells. Overexpression of MdsABC and external addition of PSPS similarly rendered the mdsABC deletion strain resistant to diamide. Diagonal gel analysis showed that PSPS treatment reduced the diamide-mediated formation of disulfide bonds, particularly in the membrane fraction of the bacteria. Salmonella infection of macrophages induced the upregulation of MdsABC expression and led to an increase of intracellular bacterial number and host cell death, similar to the effects of MdsABC overexpression and PSPS pretreatment on the mdsABC deletion strain. Our study shows that MdsABC mediates a previously uncharacterized pathway that involves PSPS as a key factor for the survival and virulence of S. Typhimurium in phagocytic cells.

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Visiting the cell biology of Salmonella infection

Cited by 54 publications

References 66 publications

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium

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