Visualization analysis of CRISPR/Cas9 gene editing technology studies

Du, Quansheng; Cui, Jie; Zhang, Chunjie; He, Ke

doi:10.1631/jzus.b1601985

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…In 2012 a major milestone in CRISPR technology revolutionized the field of molecular biology and genetic engineering, the group led by Doudna and Charpentier published an article revealing the first use of CRISPR with Cas9 endonuclease being programmed with simple RNA molecules to cleave in vitro specific sites in DNA strands (Jinek et al, 2012). Since this pioneering and subsequent works, the CRISPR / Cas system has gained wide acceptance over other systems due to its simplicity, speed and efficiency in modifying genes in any particular cell or tissue (Du et al, 2016).…”

Section: Crispr/cas System Discoverymentioning

confidence: 99%

Research Article CRISPR/Cas Class 2 systems and their applications in biotechnological processes

Mota¹,

Marques²,

Guimarães³

et al. 2020

Genet. Mol. Res.

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Section: Crispr/cas System Discoverymentioning

confidence: 99%

Research Article CRISPR/Cas Class 2 systems and their applications in biotechnological processes

Mota¹,

Marques²,

Guimarães³

et al. 2020

Genet. Mol. Res.

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CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells

Reber

Mechtersheimer

Nasif

et al. 2018

MBoC

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Computational Approaches for Elucidating Protein-Protein Interactions in Cation Channel Signaling

Zheng

Wang

et al. 2020

CDT

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Background: The lipid bilayer of the plasma membrane is impermeable to ions, yet changes in the flux of ions across the cell membrane are critical regulatory events in cells. Because of their regulatory roles in a range of physiological processes, such as electrical signaling in muscles and neurons, to name a few, these proteins are one of the most important drug targets. Objective: This review mainly focused on the computational approaches for elucidating proteinprotein interactions in cation channel signaling. Discussion: Due to continuously advanced facilities and technologies in computer sciences, the physical contacts of macromolecules of channel structures have been virtually visualized. Indeed, techniques like protein-protein docking, homology modeling, and molecular dynamics simulation are valuable tools for predicting the protein complex and refining channels with unreleased structures. Undoubtedly, these approaches will greatly expand the cation channel signaling research, thereby speeding up structure-based drug design and discovery. Conclusion: We introduced a series of valuable computational tools for elucidating protein-protein interactions in cation channel signaling, including molecular graphics, protein-protein docking, homology modeling, and molecular dynamics simulation.

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Visualization analysis of CRISPR/Cas9 gene editing technology studies

Cited by 4 publications

References 29 publications

Research Article CRISPR/Cas Class 2 systems and their applications in biotechnological processes

Research Article CRISPR/Cas Class 2 systems and their applications in biotechnological processes

CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells

Computational Approaches for Elucidating Protein-Protein Interactions in Cation Channel Signaling

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