Visualization and quantification of injury to the ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography

Gamm, Ute A.; Huang, Brendan K.; Mis, Emily K; Khokha, Mustafa K.; Choma, Michael A.

doi:10.1038/s41598-017-14670-9

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…Visualization of the mucociliary flow and cilia motion can be achieved via advanced imaging modalities, in particular, optical coherence tomography (OCT). 27 OCT imaging, however, can only provide the cross-sectional views of tissues or fluids and thus is not suitable for monitoring the two-dimensional (2D) mucociliary flow across large airway surface. On the contrary, the current study employed a microparticle tracking method, which has been extensively used in the mucociliary-related studies, 15,28 because of its proven utility in visualization of fluid movements with high spatial and temporal resolution.…”

Section: Discussionmentioning

confidence: 99%

Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissues

et al. 2023

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The airway epithelium lining the luminal surface of the respiratory tract creates a protective barrier that ensures maintenance of tissue homeostasis and prevention of respiratory diseases. The airway epithelium, unfortunately, is frequently injured by inhaled toxic materials, trauma, or medical procedures. Substantial or repeated airway epithelial injury can lead to dysregulated intrinsic repair pathways and aberrant tissue remodeling that can lead to dysfunctional airway epithelium. While disruption in the epithelial integrity is directly linked to degraded epithelial barrier function, the correlation between the structure and function of the airway epithelium remains elusive. In this study, we quantified the impact of acutely induced airway epithelium injury on disruption of the epithelial barrier functions. By monitoring alternation of the flow motions and tissue bioimpedance at local injury site, degradation of the epithelial functions, including mucociliary clearance and tight/adherens junction formation, were accurately determined with a high spatiotemporal resolution. Computational models that can simulate and predict the disruption of the mucociliary flow and airway tissue bioimpedance have been generated to assist interpretation of the experimental results. Collectively, findings of this study advance our knowledge of the structure–function relationships of the airway epithelium that can promote development of efficient and accurate diagnosis of airway tissue injury.

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Section: Discussionmentioning

confidence: 99%

Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissues

et al. 2023

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“…Moreover, for the detection of cilia flow speed, a particle tracking velocimetry OCT (PTV-OCT) is suitable. The combination between speckle variance imaging and PTV-OCT analysis required high computational power and time, which does not allow real-time analysis 21 . In addition, sensing of ciliary beat frequency by Doppler OCT (D-OCT) can measure vibrations through interferometry in living tissues but is an expensive and complex technique 22 .…”

Section: Introductionmentioning

confidence: 99%

Non-contact optical in-vivo sensing of cilia motion by analyzing speckle patterns

Duadi

Shabairou

Primov-Fever

et al. 2022

Sci Rep

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Cilia motion is an indicator of pathological-ciliary function, however current diagnosis relies on biopsies. In this paper, we propose an innovative approach for sensing cilia motility. We present an endoscopic configuration for measuring the motion frequency of cilia in the nasal cavity. The technique is based on temporal tracking of the reflected spatial distribution of defocused speckle patterns while illuminating the cilia with a laser. The setup splits the optical signal into two channels; One imaging channel is for the visualization of the physician and another is, defocusing channel, to capture the speckles. We present in-vivo measurements from healthy subjects undergoing endoscopic examination. We found an average motion frequency of around 7.3 Hz and 9.8 Hz in the antero-posterior nasal mucus (an area rich in cilia), which matches the normal cilia range of 7–16 Hz. Quantitative and precise measurements of cilia vibration will optimize the diagnosis and treatment of pathological-ciliary function. This method is simple, minimally invasive, inexpensive, and promising to distinguish between normal and ciliary dysfunction.

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“…OCT has been shown to be effective for visualizing mucus flow in both in vitro and in vivo experiments. 17 , 18 Some studies used OCT to perform direct measurements of cilia-driven fluid flow using methods such as particle tracking velocimetry 19 – 21 and OCT particle image velocimetry. 22 Here we describe a method using OCT to track particle probes in a controlled MCC-mimicking environment, giving way for investigative studies over a wide range of strain rates.…”

Section: Introductionmentioning

confidence: 99%

OCT particle tracking velocimetry of biofluids in a microparallel plate strain induction chamber

2021

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. Significance: Imaging biofluid flow under physiologic conditions aids in understanding disease processes and health complications. We present a method employing a microparallel plate strain induction chamber (MPPSIC) amenable to optical coherence tomography to track depth-resolved lateral displacement in fluids in real time while under constant and sinusoidal shear. Aim: Our objective is to track biofluid motion under shearing conditions found in the respiratory epithelium, first validating methods in Newtonian fluids and subsequently assessing the capability of motion-tracking in bronchial mucus. Approach: The motion of polystyrene microspheres in aqueous glycerol is tracked under constant and sinusoidal applied shear rates in the MPPSIC and is compared with theory. Then 1.5 wt. % bronchial mucus samples considered to be in a normal hydrated state are studied under sinusoidal shear rates of amplitudes 0.7 to . Results: Newtonian fluids under low Reynolds conditions ( ) exhibit velocity decreases directly proportional to the distance from the plate driven at both constant and oscillating velocities, consistent with Navier–Stokes’s first and second problems at finite depths. A 1.5 wt. % mucus sample also exhibits a uniform shear strain profile. Conclusions: The MPPSIC provides a new capability for studying biofluids, such as mucus, to assess potentially non-linear or strain-rate-dependent properties in a regime that is relevant to the mucus layer in the lung epithelium.

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Visualization and quantification of injury to the ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography

Cited by 6 publications

References 28 publications

Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissues

Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissues

Non-contact optical in-vivo sensing of cilia motion by analyzing speckle patterns

OCT particle tracking velocimetry of biofluids in a microparallel plate strain induction chamber

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