Visualization of lipids in cottonseeds by matrix-assisted laser desorption/ionization mass spectrometry imaging

Lu²,

Ning

et al. 2022

Analyst

Section: Resultsmentioning

confidence: 70%

Internal extractive electrospray ionization mass spectrometry for investigating the phospholipid dysregulation induced by perfluorooctanoic acid in Nile tilapia

Lu²,

Ning

et al. 2022

Analyst

“…The abscissa value and bubble size represent the degree of influence in the topology analysis, in which the larger the abscissa value and bubble size, the higher the degree of enrichment. The ordinate value and bubble color represent the p -value of the enrichment analysis; the darker the bubble color, the smaller the p -value, and the more significant the degree of enrichment [ 46 ], which indicated that glycerophospholipid metabolism was the most significant pathway in the pathway analysis results and is important to study in the heating process of oils. Glycerophospholipid metabolism mainly included the transformation between phosphatidylethanolamine (PE, C00350) and phosphatidylserine (PS, C02737), light blue means that those metabolites are not in our data and were used as background for pathway analysis.…”

Section: Resultsmentioning

confidence: 99%

Effect of Refining Degree on the Quality Changes and Lipid Oxidation of Camellia (Camellia oleifera) Oil during Heating

Wang

Zeng

et al. 2022

Foods

Refining degree has an important influence on the quality of camellia (Camellia oleifera) oil. The deterioration behaviors and lipid oxidation of three kinds of camellia oils, including camellia crude oil (CO), moderate refined oil (MRO), and refined oil (RO), during heating were investigated in this study. The results of deterioration behavior analysis showed that the oxidation degree was RO > CO > MRO. Tocopherol and polyphenolic substances in the oil might help delay oil oxidation. The lipid oxidation results indicated that the heating process had greater effects on CO and MRO than RO; it upregulated neutral lipid content and downregulated phospholipid content in terms of lipid changes and the multiplicity of differences. Glycerophospholipid metabolism was the most remarkable pathway and was important to study the heating process of refined oil. Moderate refining is good for retaining the beneficial lipids in camellia oil. The results of this study would provide a theoretical basis for camellia oil processing.

“…MALDI-MSI Analysis. The seed tissues were prepared as described in our previous work 16 with slight modifications (without using conductive tape). Briefly, cottonseeds were fixed by 4% paraformaldehyde aqueous solution for 30 min, then embedded in 10% porcine skin gelatin (Sigma Co. Ltd., Shanghai, China), and frozen at −80 °C for 2 h before cryosectioning.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Spatially Resolved Metabolomics and Lipidomics Reveal Salinity and Drought-Tolerant Mechanisms of Cottonseeds

Wang

et al. 2021

J. Agric. Food Chem.

Self Cite

In the current era of global climate change, environmental stresses, especially drought and salt, have impaired the growth and productivity of crops, e.g., cotton. Understanding the mechanisms of plants’ adaptation to these abiotic stresses is crucial to breed stress-tolerant crop species. In the present study, integrated metabolomics, lipidomics, and mass spectrometry imaging (MSI) were used to discover the spatial distribution of differential metabolites and lipids in two cottonseed cultivars with contrasting drought and salt tolerance properties. Seventeen differential metabolites and 125 differential lipids were identified. Their possible roles in augmenting stress tolerance were illustrated, which were involved in reactive oxygen species scavenging, osmotic adjustment, and cell membrane structure reconstruction. MSI analysis provided a visualization of nine differential lipids and four differential metabolites in cottonseeds with varied abundances and distributions. The results may help understand cottonseeds’ convictive metabolic and lipidomic regulatory networks in coping with salinity and drought stresses and give new insights into the stress-tolerance traits relevant to other crops.