Vitamin B<sub>6</sub> Group. Extraction procedures for the Microbiological Determination of Vitamin B<sub>6</sub>

Rabinowitz, Jesse C.; Snell, Esmond E.

doi:10.1021/ac60004a020

Cited by 58 publications

(24 citation statements)

References 19 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In conn ectio n with this heat activ ation effec t many effec tive subs tanc es were iden tifie d to date , for exam ple, the degr adat ion prod ucts of suga rs,2-.) asco rbic acid 5 ) gluc osyl glyc ine 6 ) etc. The auth ors are inter ested in the form atio n of new nitro gen containi ng com poun ds, espe ciall y ninh ydri n posi tive substanc es in the auto elav ed cultu re med ium and this pape r deal s with isola tion and iden tific ation of thes e com poun ds.…”

Section: Iso Lati On and Ide Ntif Ica Tion Of I-d Eox Y-l -(L -As Parmentioning

confidence: 99%

Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Miura

Tahara

Mizutani

1973

Agricultural and Biological Chemistry

View full text Add to dashboard Cite

Section: Iso Lati On and Ide Ntif Ica Tion Of I-d Eox Y-l -(L -As Parmentioning

confidence: 99%

Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Miura

Tahara

Mizutani

1973

Agricultural and Biological Chemistry

View full text Add to dashboard Cite

“…The relation between added thiamin in the growth medium and vitamin B, requirements for yeast growth has been studied in detail in Saccharomyces uuarum strain 4228 (Saccharomyces carlsbergensis strain 4228), an organism widely used in the assay of vitamin B, (Atkin et a/., 1943;Rabinowitz & Snell, 1947;Fukui, 1953). This yeast does not require vitamin B, as an essential growth factor, except when thiamin is added to the culture medium (Rabinowitz & Snell, 1951; Chiao & Peterson, 1956).…”

Section: Introductionmentioning

confidence: 99%

“…(1943). For the microbiological assay, the medium was steamed for 10 min at 100 "C (Atkin etal., 1943;Rabinowitz & Snell, 1947). For investigating the requirement of S .…”

Section: Introductionmentioning

confidence: 99%

“…The amount of vitamin B, in the cells or in the culture supernatant was determined using S . uvarum 4228 and with pyridoxine hydrochloride as a standard (Atkin et al, 1943;Rabinowitz & Snell, 1947). Prior to the determination, samples were acidified with 0.1 M-HC~, autoclaved at 121 "C for 3 h, and the pH was adjusted to 5.0 with 4 M-sodium acetate.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Thiamin on Vitamin B6 Synthesis in Yeasts

1982

View full text Add to dashboard Cite

The effects of exogenous thiamin on the growth yield and vitamin B, content of 18 strains of yeasts and a few strains of bacteria were examined. The addition of thiamin hardly affected the growth yield of the yeasts tested, except for two strains Saccharomyces uvarurn strain 4228 and Saccharomyces uvarum IF0 0751. In contrast, the vitamin B, content of all the yeasts tested, except Pichia membranaefaciens I F 0 0189, decreased markedly in the presence of thiamin. In S . uvarum IF0 1265, the synthesis of vitamin B, was maximally inhibited by the addition of thiamin (1.5 nmol ml-l) to the growth medium without affecting cell growth, whereas the amounts of cellular vitamin B, increased in the presence of the thiamin antagonist, pyrithiamin or oxythiamin, at concentrations that did not affect growth. When [4'-14C]pyridoxine. HC1 (0*5pgml-') was added to the growth medium at least 54% of the added isotope was incorporated into the cells during 24 h incubation, In the presence of thiamin (15 nmol ml-'), at least 32% of the added isotope was incorporated. The metabolism of [4'-14C]pyridoxine. HC1 to inactive forms having no vitamin B, activity was not stimulated by the addition of thiamin. Thus, vitamin B6 synthesis in many yeasts was affected by thiamin, whereas, in bacteria, growth yield and vitamin B, content were not affected by thiamin. I N T R O D U C T I O NThe relation between added thiamin in the growth medium and vitamin B, requirements for yeast growth has been studied in detail in Saccharomyces uuarum strain 4228 (Saccharomyces carlsbergensis strain 4228), an organism widely used in the assay of vitamin B, (Atkin et a/., 1943;Rabinowitz & Snell, 1947;Fukui, 1953). This yeast does not require vitamin B, as an essential growth factor, except when thiamin is added to the culture medium (Rabinowitz & Snell, 1951; Chiao & Peterson, 1956). Exogenous thiamin greatly depresses the vitamin B, content of this yeast, possibly by inhibiting biosynthesis (Kawasaki & Yamada, 1965;Kishi, 1969); the severe deficiency of vitamin B, in the yeast was manifest in a marked reduction in the rate of nicotinic acid biosynthesis by inhibiting the synthesis of 3-hydroxyanthranilic acid from 3-hydroxykynurenine (Kawasaki et al., 1969). Nakamura et al. (1974) reported that thiamin depressed the respiratory activity, the content of unsaturated fatty acids, especially palmitoleic acid (Nishikawa et al., 1974a) and the level of sterols (Nishikawa et al., 1974b) in the yeast, and that these effects of thiamin were abolished by the addition of pyridoxine to the growth medium. Haskell & Snell (1965) have also observed that deficiency of vitamin B, caused a marked decrease in the content of palmitoleic acid of the yeast, Hanseniaspora ualbyensis. The facts strongly support the presumption that the externally added thiamin causes primarily the inhibition of vitamin B6 biosynthesis, followed by various abnormalities in vitamin B, metabolism, so that S . uuarurn 4228 cannot grow normally without the addition of pyridoxine, pyridoxal, and pyrid...

show abstract

“…Pyridoxal phosphate is the coenzyme of many enzymes associated with protein metabolism, and if this is true for whole cells, and since pyridoxamine phosphate is the predominating member of the B6 group within the cell (Rabinowitz & Snell, 1947), the control ofthis one enzyme, pyridoxamine phosphate transaminase, could affect the whole pattern of the amino acid metabolism of the cell. That the cell has some form of control of the enzymes involved in vitamin B6 metabolism can be deduced from the fact that pyridoxal kinase also phosphorylates pyridoxine.…”

Section: Control Experimentalmentioning

confidence: 99%

Pyridoxamine-phosphate transaminase

高田¹

Springer Handbook of Enzymes

View full text Add to dashboard Cite

Vitamin B₆ Group. Extraction procedures for the Microbiological Determination of Vitamin B₆

Cited by 58 publications

References 19 publications

Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Effects of Thiamin on Vitamin B6 Synthesis in Yeasts

Pyridoxamine-phosphate transaminase

Contact Info

Product

Resources

About

Vitamin B6 Group. Extraction procedures for the Microbiological Determination of Vitamin B6

Cited by 58 publications

References 19 publications

Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Effects of Thiamin on Vitamin B6 Synthesis in Yeasts

Pyridoxamine-phosphate transaminase

Contact Info

Product

Resources

About

Vitamin B₆ Group. Extraction procedures for the Microbiological Determination of Vitamin B₆