Vitamin C from standardized water spinach extract on inhibition of cytotoxicity and oxidative stress induced by heavy metals in HepG2 cells

Yang, Ui-Jeong; Ko, Sanghoon; Shim, Soon‐Mi

doi:10.1007/s13765-013-4187-1

Cited by 21 publications

(19 citation statements)

References 25 publications

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“…MTT assay was conducted in accordance with the protocol described by Yang et al (2014). Approximately 4 × 10 3 cells were seeded in each well of the 96-well microplate.…”

Section: Measurement Of Cell Cytotoxicity By Mtt Assaymentioning

confidence: 99%

“…According to the method described in a previous study (Yang et al, 2014), the 2 ,7 -dichlorodihydrofluorescein diacetate (2 7 -DCFH-DA) assay was carried out to measure ROS generated by CSE or CSC in T98G cells. In brief, approximately 4 × 10 3 cells were seeded into 96 wells of a black microplate.…”

Section: Measurement Of Intracellular Reactive Oxygen Species (Ros)mentioning

confidence: 99%

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Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture

Kim

Cho

Choi

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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The objective of the current study was to examine oxidative stress induced by cigarette smoke extract (CSE) or cigarette smoke condensate (CSC) in human brain cells (T98G) and human brain microvascular endothelial cells (HBMEC) in mono- and co-culture systems. Cell viability of T98G cells exposed to CSC (0.05-4 mg/ml) was significantly decreased compared to CSE (0.025-20%). There were no marked differences between quantities of reactive oxygen species (ROS) generation by either CSE (2, 4, and 10%) or CSC (0.2, 0.4, and 0.8 mg/ml) treatment compared to control. However, a significant effect was noted in ROS generation following CSC incubation at 4mg/ml. Cellular integrity of HBMEC decreased to 74 and 64% within 120 h of exposure at the IC50 value of CSE and CSC, respectively. This study suggests that chronic exposure to cigarette smoking might initiate damage to the blood-brain barrier.

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“…MTT assay was conducted in accordance with the protocol described by Yang et al (2014). Approximately 4 × 10 3 cells were seeded in each well of the 96-well microplate.…”

Section: Measurement Of Cell Cytotoxicity By Mtt Assaymentioning

confidence: 99%

Section: Measurement Of Intracellular Reactive Oxygen Species (Ros)mentioning

confidence: 99%

Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture

Kim

Cho

Choi

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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“…In addition, in order to measure electron reduction, the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2 ′ -azino-bis-3ethylbenzthiazoline-6-sulfonic acid (ABTS) assays were performed according to the method described previousl. 16 wileyonlinelibrary.com/jsfa…”

Section: Measurement Of Radical Scavenging Capacitymentioning

confidence: 99%

Microbial bioconversion and processing methods enhance the phenolic acid and flavonoids and the radical scavenging capacity of Smilax china L. leaf

et al. 2015

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BACKGROUND It has been reported that Smilax china L. leaf (SCL) provided various biological functions owing to polyphenols. The objective of the current study was to assess the enhancing effect of processing methods and microbial conversions on phenolic acid and flavonoid content and radical scavenging capacity of SCL for potential applications of diverse food products. RESULTS Targeted phenolic acid (chlorogenic acid) and flavonoids (piceid and quercetin) were identified in fresh SCL using liquid chromatography–mass spectrometry. The total amount of identified phenolic acid and flavonoids was highest in steamed SCL (12.70 ± 0.12 mg g−1 on a dry matter basis, dmb). A substantial amount of chlorogenic acid (5.81 ± 0.16 mg g−1 dmb), piceid (3.96 ± 0.04 mg g−1 dmb) and quercetin (6.06 ± 0.12 mg g−1 dmb) were quantified in SCL fermented by Bacillus species, roasted and steamed, respectively (P < 0.05). The oxygen radical absorbance capacity (ORAC) value was greater in microbial fermented SCL than in others, with the exception of Saccharomyces cerevisiae and Aspergillus oryzae. However, vitamin C equivalent antioxidant capacity (VCEAC) was highest in SCL fermented by Aspergillus oryzae. CONCLUSION Results from our study suggest that the microbial fermentation processing method could improve accessibility to extraction of phenolic acids and flavonoid content and radical scavenging capacity. © 2015 Society of Chemical Industry

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“…Bioavailability is normally described as the potential amount of components in food matrix, which can be available into body (Ekmekcioglu ; Laparra and others ; Shim and Kwon ). Caco‐2 cell lines as a model of human intestines have been well recognized to predict the bioavailability of bioactive components in utilized food matrix or plants (Ekmekcioglu ; Laparra and others ; Shim and Kwon ; Yang and others ). For increasing the bioavailability of SMM, it is important to understand its intestinal transport mechanism.…”

Section: Introductionmentioning

confidence: 99%

Determination of S‐methyl‐L‐methionine (SMM) from Brassicaceae Family Vegetables and Characterization of the Intestinal Transport of SMM by Caco‐2 Cells

Song

Lee

Shim

2016

Journal of Food Science

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The objectives of the current study were to determine S-methyl-L-methionine (SMM) from various Brassicaceae family vegetables by using validated analytical method and to characterize the intestinal transport mechanism of SMM by the Caco-2 cells. The SMM is well known to provide therapeutic activity in peptic ulcers. The amount of SMM from various Brassicaceae family vegetables ranged from 89.08 ± 1.68 μg/g to 535.98 ± 4.85 μg/g of dry weight by using validated ultra-performance liquid chromatography-electrospray ionization-mass spectrometry method. For elucidating intestinal transport mechanism, the cells were incubated with or without transport inhibitors, energy source, or a metabolic inhibitor. Phloridzin and verapamil as inhibitors of sodium glucose transport protein (SGLT1) and P-glycoprotein, respectively, were not responsible for cellular uptake of SMM. Glucose and sodium azide were not affected by the cellular accumulation of SMM. The efflux ratio of SMM was 0.26, implying that it is not effluxed through Caco-2 cells. The apparent coefficient permeability (P ) of SMM was 4.69 × 10 cm/s, indicating that it will show good oral absorption in in vivo.

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Vitamin C from standardized water spinach extract on inhibition of cytotoxicity and oxidative stress induced by heavy metals in HepG2 cells

Cited by 21 publications

References 25 publications

Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture

Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture

Microbial bioconversion and processing methods enhance the phenolic acid and flavonoids and the radical scavenging capacity of Smilax china L. leaf

Determination of S‐methyl‐L‐methionine (SMM) from Brassicaceae Family Vegetables and Characterization of the Intestinal Transport of SMM by Caco‐2 Cells

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