Vitamin D status assessed by a validated HPLC method: within and between variation in subjects supplemented with vitamin D₃

Abstract

The validated HPLC method was applied in samples from human intervention studies in which subjects were supplemented with vitamin D(3). The estimated standard deviation between and within subjects is useful in the forthcoming decision on setting limits for optimal vitamin D status.

An Improved and Sensitive Method for Vitamin D3 Estimation by RPHPLC

“…In contrast to this the other HPLC methods reported the LOD ranged between 3 and 7.5 nmol/L while the LOQ ranged between 10 and 17.5 nmol/L [22][23][24][25][26].…”

Vitamin D Status and Changes in Cardiovascular Risk Factors: A Prospective Study of a General Population

“…In general, measuring serum 25(OH)D is associated with methodological concerns. As illustrated by the comparison of two HPLC methods [49], variations between methods are considerable [63,64,65,66]. There were, however, no systematic errors, and any misclassification is likely to be random and would have attenuated the associations towards the null value.…”

“…The accuracy of the HPLC method used was compared with a validated HPLC method using certified reference material (No. 968c; NIST, Gaithersburg, Md., USA) [49]. …”

Development and optimization of simplified LC–MS/MS quantification of 25-hydroxyvitamin D using protein precipitation combined with on-line solid phase extraction (SPE)

“…25-OH D exists in 2 forms: 25-hydroxyvitamin D 2 (25-OH D 2 ) and 25-hydroxyvitamin D 3 (25-OH D 3 ). Measurement of 25-OH D can be done by radioimmunoassay (RIA) [4,5], enzymoimmunoassay (EIA) [4], chemiluminescence immunoassay (CI) [4,5], high performance liquid chromatography (HPLC) [6][7][8][9][10][11][12] or LC-MS/MS [3,4,13]. Monitoring serum concentrations of 25-OH D by LC-MS/MS is now considered the gold standard because of its sensitivity and specificity [3,14,15].…”