Vitamin E-Enhanced IL-2 Production in Old Mice: Naive But Not Memory T Cells Show Increased Cell Division Cycling and IL-2-Producing Capacity

Adolfsson, Oskar; Huber, Brigitte; Meydani, Simin Nikbin

doi:10.4049/jimmunol.167.7.3809

Cited by 130 publications

(117 citation statements)

References 44 publications

Supporting

Mentioning

114

Contrasting

Order By: Relevance

“…However, recent studies indicated a direct effect of vitamin E on T cells independent of its effect on macrophages (8,9). This immunoenhancing effect of vitamin E on T cells was shown to be mediated through increasing the ability of T cells from old mice to progress through cell division cycles and to produce IL-2 (9). However, further studies are needed to elucidate the mechanisms by which vitamin E specifically enhances T cell function in the aged.…”

Section: ) Mo Et Al (2) Stimulated Purified Cd4mentioning

confidence: 97%

“…Fischer et al (39) reported a significant down-regulation of G 1 /S-specific Ccnd1 in rat liver by selenium and vitamin E deficiency, which was restored by vitamin E supplementation. Increased expression of Ccnb2, Cdc2, and Cdc6 with vitamin E in old T cells may provide the mechanism for our previous observation that vitamin E increased the ability of naive T cells from the old mice, but not the young mice, to progress through cell cycle divisions (9). Cyclin B and Cdc2 are important for entry of cells into the M phase of the cell cycle.…”

Section: Mip-1b Gm-csf Tnf-␣ Il-2 Il-4 and Egr2 Exhibited The Himentioning

confidence: 98%

“…Vitamin E exerts its effect on immune function in part by reducing macrophage PGE 2 production (4). However, recent studies indicated a direct effect of vitamin E on T cells independent of its effect on macrophages (8,9). This immunoenhancing effect of vitamin E on T cells was shown to be mediated through increasing the ability of T cells from old mice to progress through cell division cycles and to produce IL-2 (9).…”

Section: ) Mo Et Al (2) Stimulated Purified Cd4mentioning

confidence: 99%

“…This negative selection method using immunomagnetic beads was shown to result in Ն94% of the enriched cell population expressing CD3 and Ͻ1% of cells staining positive for macrophages or B cells (9). Purified T cells (1 ϫ 10 7 cells/sample) from the same animal were cultured with medium (unstimulated sample) or with 5 g/ml plate-bound anti-CD3 mAb (hamster anti-mouse, clone 145-2C11; BD Pharmingen) and 2 g/ml soluble anti-CD28 mAb (hamster anti-mouse, clone 37.51; BD Pharmingen) (stimulated sample) for 2 h.…”

Section: Cells and Culture Conditionsmentioning

confidence: 99%

See 3 more Smart Citations

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Han

Adolfsson

Lee

et al. 2006

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

T cells are vulnerable to age-associated changes. Vitamin E has been shown to improve T cell functions in the old. We studied gene expression profiles of T cells to better understand the underlying mechanisms of age and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (supplemented) ppm of vitamin E for 4 wks. Gene expression profiles of T cells were assessed using microarray analysis with/without anti-CD3/anti-CD28 stimulation. Genes associated with cytokines/chemokines, transcriptional regulation, signal transduction, cell cycle, and apoptosis were significantly up-regulated upon stimulation. Higher SOCS3 and lower growth factor independent 1 (Gfi-1) expression in old T cells may contribute to age-associated decline in proliferation. Higher Gadd45 and lower Bcl2 expression may contribute to increased apoptosis in old T cells. Vitamin E supplementation resulted in higher expression of genes involved in cell cycle regulation (Ccnb2, Cdc2, Cdc6) in old T cells. Vitamin E supplementation resulted in higher up-regulation of IL-2 expression in young and old T cells and lower up-regulation of IL-4 expression in old T cells following stimulation. These findings suggest that aging has significant effects on the expression of genes associated with signal transduction, transcriptional regulation, and apoptosis pathways in T cells, and vitamin E has a significant impact on the expression of genes associated with cell cycle and Th1/Th2 balance in old T cells. Further studies are needed to determine whether these changes are due to the effects of aging at a single-cell level or to the shift in the ratio of naïve:memory T cells with age.

show abstract

Section: ) Mo Et Al (2) Stimulated Purified Cd4mentioning

confidence: 97%

Section: Mip-1b Gm-csf Tnf-␣ Il-2 Il-4 and Egr2 Exhibited The Himentioning

confidence: 98%

Section: ) Mo Et Al (2) Stimulated Purified Cd4mentioning

confidence: 99%

Section: Cells and Culture Conditionsmentioning

confidence: 99%

See 2 more Smart Citations

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Han

Adolfsson

Lee

et al. 2006

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is known to have a significant impact on improving a variety of immune functions [50]. Supplementation with vitamin E increases the rate of lymphocyte proliferation by enhancing the ability of T cells to undergo cell division cycles [51]. The effective anti-inflammatory action of vitamin E was substantiated from observations such as the increased expression of the IL-2 gene and IL-1 receptor antagonist and the decreased expression of IL-4 following vitamin E supplementation in animal models [50].…”

Section: Vitamin Ementioning

confidence: 66%

Utility of vitamins in the prevention of type 2 diabetes mellitus and its complications: A public health perspective

Sadoun

García‐Bailo

Arora

et al. 2012

Qatar Foundation Annual Research Forum Volume 2012 Issue 1

View full text Add to dashboard Cite

Background and Objectives: The incidence of type 2 diabetes mellitus (T2DM) is increasing worldwide, and certain sub-populations appear especially vulnerable to developing the disease. In Qatar, the prevalence of T2DM in the adult population is approximately twice that in the USA. Qatar is ranked sixth in the world for diabetes prevalence. Risk of disease in the country is increasing with an apparent doubling of incidence in children from 1997 to 2007. To reduce T2DM risk and progression, preventative strategies need to be implemented on a population-wide scale with minimal cost and effort and without adverse effect. Our objective was to examine the utility of micronutrients in the prevention of type 2 diabetes and its complications and to evaluate the pathway(s) through which these dietary factors exert their effect. Methods: We searched the available literature linking oxidation, inflammatory signaling pathways, and micronutrient intake to T2DM. Results: The rise of T2DM rates is thought to be due, in part, to genetic factors that interact with lifestyle factors including, but not limited to, poor nutrition, lack of physical activity and obesity. However, chronic low-grade inflammation resulting from oxidative stress and imbalances in the innate immune system has been also linked to the pathogenesis of T2DM. Reducing inflammation via modulation of oxidative stress and the innate immune response could lead to a status of improved insulin sensitivity and delayed disease onset. Dietary supplementation with anti-inflammatory and antioxidant micronutrients might present a feasible strategy toward the prevention of T2DM at the population level, particularly in vulnerable sub-populations. Conclusion: Several lines of evidence support the concept of employing micronutrients as a preventative measure for T2DM via attenuating inflammation and modulating oxidative stress. This approach may be introduced as part of a population-based strategy to curb the rising incidence of T2DM in Qatar and facilitate developing public health policies and actions for disease prevention and control.

show abstract

Vitamin E inhibits IL-4 gene expression in peripheral blood T cells

et al. 2002

View full text Add to dashboard Cite

IL‐4 plays a pivotal role in the development of allergic inflammation via induction of IgE isotype switching, increase of IgE receptor expression, promoting Th2 cell differentiation, and stimulating several genes involved in atopic disorders. Previous studies in human and mouse models have shown that high vitamin E intake correlates with low IgE concentration and reduced prevalence of allergic reactions. We, therefore, investigated the mechanism of the vitamin E effect on T cells. We show here that the natural free radical scavenger vitamin E suppresses IL‐4 protein levels in humanperipheral blood T cells in a dose‐dependent manner. The effect of vitamin E on IL‐4 expression occurs at the mRNA level. Vitamin E has been implicated in inhibition of DNA binding and function of the redox‐regulated transcription factors NF‐κB and AP‐1. Investigation of the molecular mechanism by which vitamin E suppresses IL‐4 transcription shows that it blocks binding of transcription factors to two important IL‐4 promoter binding sites for NF‐κB and AP‐1 and interferes with promoter activity upon T cell activation. Our data provide molecular evidence supporting the beneficial effect of dietary vitamin E on atopic disorders.

show abstract

Vitamin E-Enhanced IL-2 Production in Old Mice: Naive But Not Memory T Cells Show Increased Cell Division Cycling and IL-2-Producing Capacity

Cited by 130 publications

References 44 publications

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Utility of vitamins in the prevention of type 2 diabetes mellitus and its complications: A public health perspective

Vitamin E inhibits IL-4 gene expression in peripheral blood T cells

Contact Info

Product

Resources

About