Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells

Kim, Soonhee; Park, Hee-Sook; Lee, Myoung-Su; Cho, Yong-Jin; Kim, Young-Sup; Hwang, Jin‐Taek; Sung, Mi Jeong; Kim, Myung Sunny; Kwon, Dae Young

doi:10.1016/j.bbrc.2008.04.188

Cited by 69 publications

(46 citation statements)

References 23 publications

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“…31 Moreover, vitisin A could inhibit 3T3L1 adipocyte differentiations through p21-dependent and Rb-dependent cell cycle arrest, which resulted in suppressions of preadipocyte proliferation. 32 Taken together, these results suggested that VTT ethanol extract or vitisin A might reduce serum lipid levels and inhibition against preadipocyte differentiations to suppress the development of obesity. In the present study VTT-Et might act to inhibit α-glucosidase and DPP-IV activities to ameliorate impaired glucose tolerance rather than weight loss in obese rats.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 79%

Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

Lin

Chen

et al. 2015

J. Agric. Food Chem.

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Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 79%

Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

Lin

Chen

et al. 2015

J. Agric. Food Chem.

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“…RES, an activator of Sirt1 [Picard et al, 2004;Backesjo et al, 2006], has various pharmacological effects including protection of cells from lipid accumulation, chemoprevention, immunomodulation, antiproliferation and promotion of differentiation [Pervaiz, 2003]. Previous studies have shown that RES reduces blood TG content and stimulates free fatty acid (FFA) release, lowers blood fat, and inhibits adipocyte differentiation and fat accumulation by activation of Sirt1 [Picard et al, 2004;Kim et al, 2008]. Based on the previous reports and the results of this study, Sirt1 appears to downregulate porcine FABP3 gene expression in porcine adipocytes, a supposition further supported by the results of the synergistic siRNA/RES findings reported here.…”

Section: Discussionmentioning

confidence: 99%

Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Shan

Ren

et al. 2009

J of Cellular Biochemistry

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To investigate whether Sirt1 could modulate fatty acid-binding protein 3 (FABP3), we treated porcine adipocytes either with the Sirt1 inhibitor nicotinamide (NAM), with the Sirt1 activator resveratrol (RES), or by knockdown of Sirt1 by Sirt1-siRNA. NAM or knockdown with Sirt1-siRNA significantly inhibited Sirt1 mRNA expression, while increasing FABP3 mRNA levels. RES or RES + Sirt1-siRNA treatments further proved that Sirt1 negatively regulated FABP3 gene expression in adipocytes. We also found a similar Sirt1 regulation pattern for PPAR gamma to that of FABP3 in adipocytes. Furthermore, NAM/RES + PPAR gamma-siRNA treatments showed that Sirt1 may regulate the FABP3 gene expression partly through the PPAR gamma-mediated signals. In summary, Sirt1 regulates the expression of FABP3 gene in adipocytes, and PPAR gamma apparently plays an important role in this process.

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“…A plenty of adipokines were consequently released to play multifaceted roles through both central and peripheral mechanisms due to the excessive increase of sheer number and volume of adipocytes. Targeting on adipocyte differentiation became one approach among the anti-obesity and related diseases strategies targeting on strong rise in fat storage and secretion of adipokine(s) [Bray and Tartaglia, 2000;Rosen and Spiegelman, 2006;Trujillo and Scherer, 2006;Kim et al, 2008]. Previous studies demonstrated that many transcription factors and metabolism enzymes are altered during various stages of adipogenesis and play roles in adipocyte differentiation (e.g., CCAAT/ enhancer binding proteins (C/EBPs), cAMP response elementbinding protein (CREB), peroxisome proliferator-activated receptor (PPAR)-gamma, fatty acid synthase (FAS), pyruvate carboxylase (PCX), superoxide dismutase (SOD), and lipoprotein lipase (LPL) that are differentially expressed during adipocyte differentiation).…”

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confidence: 99%

Comparative proteome analysis of 3T3-L1 adipocyte differentiation using iTRAQ-coupled 2D LC-MS/MS

et al. 2011

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Adipose tissue is critical in obesity and type II diabetes. Blocking of adipocyte differentiation is one of the anti-obesity strategies targeting on strong rise in fat storage and secretion of adipokine(s). However, the molecular basis of adipocyte differentiation and its regulation remains obscure. Therefore, we exposed 3T3-L1 cell line to appropriate hormonal inducers as adipocyte differentiation model. Using iTRAQ-coupled 2D LC-MS/MS, a successfully exploited high-throughput proteomic technology, we nearly quantitated 1,000 protein species and found 106 significantly altered proteins during adipocyte differentiation. The great majority of differentially expressed proteins were related to metabolism enzymes, structural molecules, and proteins involved in signal transduction. In addition to previously reported differentially expressed molecules, more than 20 altered proteins previously unknown to be involved with adipogenic process were firstly revealed (e.g., HEXB, DPP7, PTTG1IP, PRDX5, EPDR1, SPNB2, STEAP3, TPP1, etc.). The partially differential proteins were verified by Western blot and/or real-time PCR analysis. Furthermore, the association of PCX and VDAC2, two altered proteins, with adipocyte conversion was analyzed using siRNA method, and the results showed that they could contribute considerably to adipogenesis. In conclusion, our data provide valuable information for further understanding of adipogenesis.

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Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells

Cited by 69 publications

References 23 publications

Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Comparative proteome analysis of 3T3-L1 adipocyte differentiation using iTRAQ-coupled 2D LC-MS/MS

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