Volatile anesthetic sevoflurane suppresses lung cancer cells and miRNA interference in lung cancer cells

Wang, Le; Wang, Tiao; Gu, Jiaqi; Su, Huibin

doi:10.2147/ott.s171672

Cited by 26 publications

(26 citation statements)

References 25 publications

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“…For example, volatile anestheticsSev or desflurane inhibits cell invasion by downregulation of matrix metallopeptidase 9 (MMP9) in colorectal cancer [6]. Moreover, Sev suppresses migration and invasion but promotes apoptosis in lung cancer by varying pathway [7,8]. Importantly, Sev exposure reduces migration and MMP2 activity in U87-MG glioma cells [9].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16

Zhang

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background: Glioma is the most common brain tumor with poor prognosis all over the world. Anesthetics have been demonstrated to have important impacts on cell migration and invasion in different cancers. However, the underlying mechanism that allows anesthetics-mediated progression of glioma cells remains elusive. Methods: Sevoflurane (Sev), a class of common anesthetics, was used to expose to U87-MG and U251 cells. The expressions of microRNA-146b-5p (miR-146b-5p) and matrix metallopeptidase 16 (MMP16)were measured by quantitative real-time polymerase chain reaction or western blot. Transfection was performed in glioma cells with miR-146b-5p inhibitor, inhibitor negative control, MMP16 overexpression vector, empty vector, small interfering RNA against MMP16 or scramble. Cell migration and invasion were analyzed by the trans-well assay. The interaction between miR-146b-5p and MMP16 was explored by luciferase activity and RNA immunoprecipitation assays. Results: Sev treatment inhibited migration and invasion of glioma cells. The expression of miR-146b-5p was enhanced and MMP16 protein was decreased in glioma cells after exposure of Sev. Knockdown of miR-146b-5p or overexpression of MMP16 reversed Sev-induced inhibition of migration and invasion of glioma cells. Moreover, MMP16 was indicated as a target of miR-146b-5p and its silencing attenuated the regulatory role of miR-146b-5p abrogationin Sev-treated glioma cells. Conclusion: Sev impeded cell migration and invasion through regulating miR-146b-5p and MMP16 in glioma, indicating a novel theories foundation for the application of anesthetics like Sev in glioma.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16

Zhang

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Results from several studies show inconsistent effects of volatile anesthetics on cell proliferation, apoptosis and metastatic potential. In A549 lung cancer cells, incubation with sevoflurane enhanced apoptosis, increased apoptotic bodies, impaired DNA integrity (14) and caused changes in apoptosis-related miRNAs (15). Liang and colleagues showed apoptosis induction and inhibition of cell proliferation, as well as anti-invasive and anti-migratory effects by sevoflurane via down-regulation of expression of matrix metalloproteinase (MMP)-2 and -9 (16).…”

Section: Discussionmentioning

confidence: 99%

“…In preclinical studies, incubation time ranged from 30 min (15) to 48 h (26); in many studies incubation times from 2 to 6 h were used (14,20,23,24,27). Concentrations used ranged from 1.7 vol% (16) to 6 vol% for sevoflurane (26) and from 6.6 vol% (28) to 12 vol% for desflurane (26).…”

Section: Discussionmentioning

confidence: 99%

Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells In Vitro

et al. 2019

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Background/Aim: For patients undergoing cancer surgery, the risk for cancer progression is enhanced during the perioperative period. To what extent the type of anesthetic can affect the metastatic process and finally the outcome of patients with cancer is under debate. For this reason, the aim of this study was to investigate the effects of the volatile anesthetics sevoflurane and desflurane on colon cancer cells in vitro. Materials and Methods: SW480 colon carcinoma cells were exposed for 3 or 6 h to sevoflurane (1 or 2.5 vol%) or desflurane (6 or 12 vol%). Cell cycle distribution was analyzed by flow cytometry after a 24-72 h recovery and apoptosis was detected by annexin V staining after a 0-48 h recovery. Viability was tested by measuring ATP content after 0 and 24 h recovery. Results: Treatment with sevoflurane or desflurane caused no or only slight changes in cell-cycle distribution and apoptosis rate. Desflurane at 12vol% significantly reduced cell viability by 17±25% and 11±22% after 3 and 6 h incubation and 24 h recovery, respectively, while 2.5 vol% sevoflurane slightly increased viability. Conclusion: At clinically relevant concentrations, sevoflurane and desflurane had only slight effects on SW480 colon cancer cells in vitro.

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“…Sevoflurane is a commonly used inhalation anesthetic drug. Previous studies have shown that sevoflurane could play a role in a variety of tumors, such as colon cancer, 10 breast cancer, 11 lung cancer 12 and cervical cancer. 13 These reports suggested that sevoflurane had potential clinical application value in malignant tumor, but its effect on malignant behavior of OC cells and mechanism had not been completely clarified.…”

Section: Introductionmentioning

confidence: 99%

<p>Sevoflurane Inhibits Proliferation and Invasion of Human Ovarian Cancer Cells by Regulating JNK and p38 MAPK Signaling Pathway</p>

Kang

Wang

2019

DDDT

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Aim: Sevoflurane is a halogen inhaled anesthetic, and we aimed to probe the effect of sevoflurane on proliferation and invasion of human ovarian cancer (OC) and its mechanism. Methods: OC cell lines were divided into 4 groups including control, sevoflurane low concentration (1.7%), medium concentration (3.4%) and high concentration (5.1%) groups. Flow cytometry and MTT assay were, respectively, employed to detect the cell apoptosis and proliferation. Transwell assay was applied to measure the cell migration and invasion viability. The gene and protein expressions were assessed using qRT-PCR and Western blot. The expressions of MAPK signaling pathway-related proteins were evaluated by Western blot. The p38 and JNK inhibitors were, respectively, added into the high concentration group to analyze the relationship between sevoflurane and modulatingmitogen-activated protein kinase (MAPK) pathway in OC. Nude mice models were constructed to explore the effect of sevoflurane on OC tumor growth in vivo. Results: Sevoflurane inhibited OC proliferation in vitro and in vivo. It could also promote OC cell apoptosis in a dose-dependent manner. Sevoflurane suppressed the OC cell migration and invasion, and these effects were positively correlated with the dose of sevoflurane. Moreover, sevoflurane treatment inhibited the expressions of PCNA, Twist, cleavedcaspase-3/caspase-3, MMP-2 and MMP-9. In addition, sevoflurane repressed the phosphorylation of JNK and p38 MAPK. When the MAPK pathway was interdicted, the cell proliferation, apoptosis, migration and invasion activity were recovered after sevoflurane treatment. Conclusion: Sevoflurane affected cell biological activities in OC by regulating JNK and p38 MAPK signaling pathway.

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Volatile anesthetic sevoflurane suppresses lung cancer cells and miRNA interference in lung cancer cells

Cited by 26 publications

References 25 publications

RETRACTED ARTICLE: Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16

RETRACTED ARTICLE: Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16

Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells In Vitro

<p>Sevoflurane Inhibits Proliferation and Invasion of Human Ovarian Cancer Cells by Regulating JNK and p38 MAPK Signaling Pathway</p>

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