Hydrodistilled oil obtained from the aerial parts of Artemisia scoparia Waldst. & Kit. was analysed by capillary GC and GC-MS. The volatile oil of the 10-15 cm high herb was composed mainly of eight monoterpenes (ca. 68.5%), and the predominant constituents were β-myrcene (24.4%), γ-terpinene (18.3%), neral (12.5%) and cis-p-mentha-2-en-1-ol (5.1%). Among seven sesquiterpenes (6.9%) present in the oil, the prominent one was β-caryophyllene (3.4%). p-Cymene (17.4%) and p-cymene-8-ol were the characterized aromatic compounds in the volatile oil. Three aliphatic constituents, identified as n-octane, n-heptadecane and n-eicosane, occurred in trace
ExperimentalFresh aerial parts of A. scoparia were collected from the cultivated species grown in the Herbal garden of Jamia Hamdard, New Delhi, India. The plant material was identified and authenticated by Dr. M. P. Sharma, Department of Botany, Jamia Hamdard, New Delhi, India. A voucher specimen (No. DBT-01-03) is deposited in the herbarium of the Department of Pharmacognosy and Phytochemistry, Jamia Hamdard.
Isolation of OilThe air-dried plant material was hydrodistilled in an all-glass apparatus according to the method recommended by the British Pharmacopoeia, 1988. The pale yellow oil was dried over anhydrous sodium sulphate and stored at 4°C in the dark. The yield was 0.65% based on the dry weight of sample.
GC AnalysisAnalytical GC with FID detector was carried out on a Varian 3300 gas chromatograph fitted with Silicone DB-1 capillary column (30 m × 0.25 mm i.d., film thickness 0.25 µm). The carrier gas was helium at a flow rate of