Voltammetric determination of vitamin B6 (Pyridoxine) using multi wall carbon nanotube modified carbon-ceramic electrode

Abstract

A simple procedure was developed to prepare a carbon-ceramic electrode (CCE) modified with multi wall carbon nanotube (MWCNT). The electrochemical behavior of pyridoxine was investigated on the obtained electrode in phosphate buffer solution (PBS), pH 7.0. During oxidation of pyridoxine on the MWCNT/CCE, one irreversible anodic peak at E p = 716 mV vs. SCE appeared. Cyclic voltammetric study indicated that the oxidation process is irreversible and diffusion controlled. The number of exchanged electrons in the electro-oxidation process was obtained, and the data indicated that pyridoxine is oxidized via two oneelectron steps. The results revealed that MWCNT promotes the rate of oxidation by increasing the peak current, so that pyridoxine is oxidized at lower potentials, which thermodynamically is more favorable compared with on the bare CCE. A sensitive, simple and time-saving differential pulse voltammetric procedure was developed for the analysis of pyridoxine. Using the proposed method, pyridoxine can be determined with a detection limit of 95 nM. The applicability of the method to direct assays of some commercial pharmaceutical samples is described.

Validated Spectrophotometric Method to Assay of B₆ and B₃ Vitamins in Pharmaceutical Forms Using Potassium Iodide and Potassium Iodate

“…Addition of acidic or basic solutions affects in a negative way on the analytical method. Both vitB 9 , vitB 12 present in the formulations do not interfere with the assay procedures. …”

“…VitB 3 is required for cell respiration, for proper circulation and healthy skin, for the functioning of the nervous system and for the normal secretion of bile and stomach fluids Figure 1: Chemical structures of a) Vitamin B 6 ., b) Vitamin B 3 . [1] Many methods have been developed for the determination of vitB 6 in pharmaceutical formulations include spectrophotometry [2][3][4][5][6], spectrofluorimetry [7], voltammetry [8][9][10][11][12], potentiometry [13,14]. vitB 3 has been determined in pharmaceutical formulations by chemical luminescence [15], spectrophotometry [16].…”

Sensitive Determination of Acetaminophen in the Presence of Dopamine and Pyridoxine Facilitated by their Extent of Interaction with Single‐walled Carbon Nanotubes

“…Three forms of PY are available which are known as, pyridoxal, pyridoxol and pyridoxamine . PY is also necessary for physical and mental growth of the individual . It is reported that vitamin B 6 imposes major effects on gene modulation, sexual behaviour, and immune modulation in HIV‐1 infection .…”

“…It is reported that vitamin B 6 imposes major effects on gene modulation, sexual behaviour, and immune modulation in HIV‐1 infection . Insufficient concentration level of PY in body leads to illness and various sicknesses such as hypertension, cardiovascular problems, hyperlipidemia, obesity and anaemia . Hence, accurate PY determination is essential in human health diagnostics.…”

A New Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Pyridoxine Hydrochloride and Meclizine Hydrochloride in Pharmaceutical Solid Dosage Forms

“…The pharmacopeias describe potentiometric, spectrophotometrics and HPLC method for the determination of PYH and MEH individually from the bulk and tablet dosage form. No reversed-phase high-performance liquid chromatography (RP-HPLC) method is reported in pharmacopeias for the simultaneous estimation of PYH and MEH from their combined formulation, though there are various methods for the determination of PYH and MEH by spectrophotometric [7][8][9][10][11][12], voltammetric [13], HPLC [14][15][16], electrophoresis [17], GLC [18], HPTLC [19], and TLC [20] methods in different pharmaceutical dosages forms. The present work describes the development and validation of stability indicating RP-HPLC method, which can quantify PYH and MEH simultaneously in pharmaceutical solid dosage form.…”