Voltammetry detection of copper in high specific activity 64Cu

A Chromatographic Technique To Investigate the Lability of Copper Complexes under Steady-State Conditions Using High Specific Activity 64Cu

“…Ionic strengths and pHs of the solutions were adjusted with sodium chloride (J. T. Baker, Deventer, The Netherlands), MES buffer (β-morpholinoethanesulfonic acid monohydrate, Sigma-Aldrich, Steinheim, Germany), and sodium hydroxide (J. T. Baker, Deventer, The Netherlands). High specific activity 64 Cu 2+ ( t 1/2 = 12.7 h; γ 0.511 MeV) was prepared according to the method described by van Elteren et al via the 64 Zn(n,p) 64 Cu reaction. A high-purity ZnO target (Grade A1 (99.9995%), Johnson Matthey, Materials Technology, U.K.) was irradiated in the reactor facility of the Interfaculty Reactor Institute (Delft, The Netherlands), followed by a chemical separation of Cu from Zn, yielding a 500 μL aqueous solution with a specific activity of >8 × 10 15 Bq·g -1 Cu.…”

Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli

“…High-specific-activity 64 Cu (t 1/2 = 12.7 h) was prepared as described earlier [18]. In short, 50 mg zinc oxide was irradiated with fast neutrons (1.9 9 10 19 m -2 s -1 ) at the Reactor Institute Delft (Delft, The Netherlands) for 12 h. During this irradiation, 64 Cu was produced from 64 Zn by the nuclear exchange of a proton for a neutron.…”

Production of copper-64 using a hospital cyclotron: targetry, purification and quality analysis

“…While many authors have described the production of 64 Cu [ 27 ], few report analytical details of these contaminants, and those reports that do provide such information focus largely on radionuclidic purity (typically by gamma spectroscopy) and molar activity of 64 Cu [ 28 ]. The latter is most often measured by spectrophotometric or HPLC determination using copper chelators such as 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetraacetic acid (TETA) [ 23 , 28 – 30 ] or diacetyl bis(N4-methylthiosemicarbazone (ATSM) [ 31 ], or by voltammetry [ 32 ], inductively-coupled plasma mass spectrometry (ICP-MS) [ 28 ] or ion chromatography [ 27 , 28 ]. Other metal contaminants that may influence labelling and molar activity of the final tracer or indeed interfere with molar activity measurement are typically neglected.…”

“…(ATSM) [31], or by voltammetry [32], inductively-coupled plasma mass spectrometry (ICP-MS) [28] or ion chromatography [27,28]. Other metal contaminants that may influence labelling and molar activity of the final tracer or indeed interfere with molar activity measurement are typically neglected.…”