Volume and concentration dosing in picolitres using a two-channel microfluidic AFM cantilever

Verlinden, Eleonoor; Madadelahi, Masoud; Sarajlic, Edin; Shamloo, Amir; Engel, Andréas; Staufer, U.; Ghatkesar, Murali Krishna

doi:10.1039/c9nr10494a

Cited by 14 publications

(9 citation statements)

References 39 publications

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“…A recent detailed work has reported the employment of a hollow tip dispensing of femtolitre to picolitre droplets through an engineered cantilever with two microchannels. 92 The peculiarity of the system is that two fluidic reservoirs are connected to two different microchannels which are in turn connected at the same hollow tip. The authors investigated the effect of applied pressure on the two liquid reservoirs, in the range from a negative difference of 500 mbar to a positive difference of 500 mbar on the fluid with respect to ambient pressure.…”

Section: Towards Single-cell Intracellular Deliverymentioning

confidence: 99%

“Writing biochips”: high-resolution droplet-to-droplet manufacturing of analytical platforms

Arrabito

Gulli

Alfano

et al. 2022

Analyst

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Section: Towards Single-cell Intracellular Deliverymentioning

confidence: 99%

“Writing biochips”: high-resolution droplet-to-droplet manufacturing of analytical platforms

Arrabito

Gulli

Alfano

et al. 2022

Analyst

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“…A hollow silicon nitride (Si3N4) tip on a silicon dioxide (SiO2) fluidic cantilever and an array of these devices was developed for high throughput applications 73 . A silicon nitride fluidic cantilever without a tip was reported by Schön et al 74 and in a later effort, a tip with a submicron aperture was included 75 . Other efforts included the use of flexible materials like SU-8 as a cantilever material 70,[76][77][78] .…”

Section: Afm For Ev Analysesmentioning

confidence: 99%

Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies

Qiu

Chien

Maroulis

et al. 2021

Preprint

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In this article, a review of the application of atomic force microscopy (AFM) for the analyses of extracellular vesicles is presented. This information is then extended to include fluidic Atomic Force Microscopy (fluidic AFM) applications. Fluidic AFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. AFM applications involving single cell and extracellular vesicle studies, colloidal force spectroscopy, and single cell adhesion measurements are discussed. In this review, new results are offered, using fluidic AFM, to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces (LBFs) of endothelial or epithelial cells in a confluent cell monolayer in appropriate physiological environment, and (3) the ease of measurement of vertical binding force (VBFs) of intercellular adhesion between heterogeneous cells. Finally, key applications are discussed that include extracellular vesicle absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

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“…Platinum resistance was also determined using an external LCR meter. Verlinden et al 25 proposed a two-channel microfluidic AFM cantilever to enable multiple reagents dosing inside another liquid environment as an application to highlight the importance of flow metric in (bio) chemical reactions. This device was capable of controlling flow rates ranging from femtoliters to pico-liters per second.…”

Section: Introductionmentioning

confidence: 99%

A suspended polymeric microfluidic sensor for liquid flow rate measurement in microchannels

Mohammadamini

Shahrouzi

Samadi

2022

Sci Rep

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In this study, a microfluidic cantilever flow sensor was designed and manufactured to monitor liquid flow rate within the range of 100–1000 µl/min. System simulation was also performed to determine the influential optimal parameters and compare the results with experimental data. A flowmeter was constructed as a curved cantilever with dimensions of 6.9 × 0.5 × 0.6 mm3 and a microchannel carved with a CO2 laser inside the cantilever beam. The fabrication substance was Polydimethylsiloxane. Different flow rates were injected using a syringe pump to test the performance of the flowmeter. Vertical displacement of the cantilever was measured in each flowrate using a digital microscope. According to the results, the full-scale overall device accuracy was up to ± 1.39%, and the response time of the sensor was measured to be 6.3 s. The microchip sensitivity was 0.126 µm/(µl/min) in the range of measured flow rates. The sensor could also be utilized multiple times with an acceptable error value. The experimental data obtained by the constructed microchip had a linear trend (R2 = 0.995) and were of good consistency with simulation results. Furthermore, according to the experimental and the simulation data, the initially curved cantilever structure had a higher bending and sensitivity level than a perfectly straight cantilever construction.

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Volume and concentration dosing in picolitres using a two-channel microfluidic AFM cantilever

Abstract: We introduce a two-channel microfluidic atomic force microscopy cantilever that can be used both for nanomechanical sensing and to manipulate liquids at the rate of femto-litres per second through nanoscale apertures near the cantilever tip apex.

Cited by 14 publications

References 39 publications

“Writing biochips”: high-resolution droplet-to-droplet manufacturing of analytical platforms

“Writing biochips”: high-resolution droplet-to-droplet manufacturing of analytical platforms

Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies

A suspended polymeric microfluidic sensor for liquid flow rate measurement in microchannels

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