Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories (Adv. Mater. 15/2022)

Bernal, Paulina Núñez; Bouwmeester, Manon C.; Madrid‐Wolff, Jorge; Falandt, Marc; Florczak, Sammy; Rodriguez, Nuria Ginés; Li, Yang; Größbacher, Gabriel; Samsom, Roos‐Anne; Wolferen, Monique van; Laan, Luc J. W. van der; Delrot, Paul; Loterie, Damien; Malda, Jos; Moser, Christophe; Spee, Bart; Levato, Riccardo

doi:10.1002/adma.202270112

Cited by 16 publications

(54 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iod is a nontoxic, water‐soluble, non‐ionic radiocontrast agent that had also been recently used as RI‐matching compound in VP (Figure 1B). [ 30 ] The Iod stock solution (60%) has a significantly higher RI (1.4310) compared to the photoresin and its addition resulted in a linear increase in the photoresins RI up to 1.4195 for Iod 50% (Figure 1B). As described by Kip et al., [ 47 ] the self‐focusing effect due to OMI appears, for a given laser coherence length and intensity, only if the non‐linearity (change in RI due to crosslinking) exceeds a certain threshold.…”

Section: Resultsmentioning

confidence: 99%

“…[ 26,27 ] Recent studies have demonstrated the possibility to create hollow, perfusable structures, using materials from glass to biopolymers and possibly targeting mesoscale vasculature. [ 28–31 ] However, as all the methods described above, also VP falls short in covering resolution range from µm/sub‐µm up to cm, thus currently limiting its application to microfluidic constructs with features >100–200 µm.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiscale Hybrid Fabrication: Volumetric Printing Meets Two‐Photon Ablation

Rizzo

Rütsche

Liu

et al. 2023

Adv Materials Technologies

View full text Add to dashboard Cite

perfusable channels. In the past decades, microfluidic technology has mostly relied on photolithography and soft lithography applied to materials like glass and elastomers (i.e., PDMS), and has been largely limited to 2D devices. [3,6] In recent years, 3D printing has emerged as a powerful tool to generate highly complex, freeform structures with enhanced functionalities, leading to great advances in various fields from photonic crystals to tissue scaffolds. [7][8][9][10][11][12] While two-photon stereolithography has represented the method of choice for the fabrication in the nm to µm scale, [8,9] for constructs with channels in the millimeter to centimeter scale various printing methods have been explored, such as direct ink writing, [13,14] embedded printing with fugitive inks (FRESH, SWIFT), [15][16][17][18] laser-sintering of sacrificial carbohydrate templates, [19] and digital light processing (DLP). [20][21][22] These technologies have particularly contributed to the tremendous progress in the design of increasingly complex vasculature networks for biomedical applications. [18,[23][24][25] More recently, a novel light-based fabrication method termed volumetric printing (VP) has emerged as a promising technology for such applications, enabling the printing of complex centimeter-sized models within seconds. [26,27] Recent studies have demonstrated the possibility to create hollow, perfusable structures, using materials from glass to biopolymers and possibly targeting mesoscale vasculature. [28][29][30][31] However, as all the methods described above, also VP falls short in covering resolution range from µm/sub-µm up to cm, thus currently limiting its application to microfluidic constructs with features >100-200 µm.Another light-based method named two-photon ablation (2PA) instead offers complementary capabilities, being limited in printing time and construct size, but reaching the highest resolution of any biofabrication method (≤1 µm). [8] 2PA is based on multiphoton ionization induced by high-intensity pulsed lasers, [32,33,34] and has been explored for a variety of applications, from "nanosurgery" to form cell-instructive microchannels. [35][36][37][38][39][40][41] Here, we show for the first time the hybrid/combined use of VP and 2PA printing technology to reproduce a multiscale Multiscale printing of 3D perfusable geometries holds great potential for a range of applications, from microfluidic systems to organ-on-a-chip. However, the generation of freeform designs spanning from centimeter to micrometer features represents an unmet challenge for a single fabrication method and thus may require the convergence of two or more modalities. Leveraging the great advances in light-based printing, herein a hybrid strategy is introduced to tackle this challenge. By combining volumetric printing (VP) and high-resolution two-photon ablation (2PA), the possibility to create multiscale models with features ranging from mesoscale (VP) to microscale (2PA) is demonstrated. To successfully combine these two methods, micro...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiscale Hybrid Fabrication: Volumetric Printing Meets Two‐Photon Ablation

Rizzo

Rütsche

Liu

et al. 2023

Adv Materials Technologies

View full text Add to dashboard Cite

show abstract

“…They use the recently developed volumetric bioprinting method for the fabrication of organoids, which capture the key functions of the human liver and undergo hepatocytic differentiation with albumin synthesis, liver-specific enzyme activity and have remarkably acquired a native-like cell polarization. 36 transmission. The nerve was then implanted into a synchronous bionic robotic hand system where it showed a real-time high fidelity and high throughput information interaction, both under deformation and cryogenic temperatures.…”

Section: Biomaterials Research In Advanced Materials (Ekaterina Peret...mentioning

confidence: 99%

The Year 2022 in biomaterials research: A perspective from the editors of six leading journals

Stegemann

Wagner

Göbel

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

The field of biomaterials science is highly active, with a steadily increasing number of publications and new journals being founded. This article brings together contributions from the editors of six leading journals in the area of biomaterials science and engineering. Each contributor highlights specific advances, topics, and trends that have emerged through the publications in their respective journal in the calendar year 2022. It presents a global perspective on a wide range of material types, functionalities, and applications. The highlighted topics include a diversity of biomaterials; from proteins, polysaccharides, and lipids to ceramics, metals, advanced composites, and a variety of new forms of these materials. Important advances in dynamically functional materials are presented, including a range of fabrication techniques such as bioassembly, 3D bioprinting and microgel formation. Similarly, several applications are highlighted in drug and gene delivery, biological sensing, cell guidance, immunoengineering, electroconductivity, wound healing, infection resistance, tissue engineering, and treatment of cancer. The goal of this paper is to provide the reader with both a broad view of recent biomaterials research, as well as expert commentary on some of the key advances that will shape the future of biomaterials science and engineering.

show abstract

“…When compared to Matrigel, which has unstable physical properties, biomimetic hydrogels have the advantage of being more controllable and precise as bio-inks. Some biomimetic hydrogels have been applied as bioinks in 3D printing, including liver-like metabolic biofactories, 70 templates for growing soft tissue organoids, 71 fully synthetic vascularisation platform 68 and a crosslinkable microgel composite matrix bath for embedded bioprinting of perfusable tissue constructs and sculpting of solid objects. 72 Overall, these innovative techniques for creating complex tissue structures should be able to maintain a high cell viability and functionality, which could have important implications for tissue engineering.…”

Section: Perspectivementioning

confidence: 99%