Vv<scp>AMP</scp>2, a grapevine flower‐specific defensin capable of inhibiting <i><scp>B</scp>otrytis cinerea</i> growth: insights into its mode of action

Nanni, Valentina; Schumacher, Julia; Giacomelli, L.; Brazzale, D.; Sbolci, L.; Moser, C.; Tudzynski, Paul; Baraldi, Elena

doi:10.1111/ppa.12170

Cited by 18 publications

(10 citation statements)

References 37 publications

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“…These proteins belong to PR-5 protein family, well known to inhibit fungal growth including B. cinerea (Monteiro et al, 2003; González et al, 2017). Notably, we found defensin and lipid transfer proteins (belonging to PR-12 and 14 protein family, in respective order), functioning as antimicrobial peptides (Garcia-Olmedo et al, 1995; Ganz, 2003; Nanni et al, 2014), downregulated in white fruits.…”

Section: Resultsmentioning

confidence: 89%

Transcriptome Profiles of Strawberry (Fragaria vesca) Fruit Interacting With Botrytis cinerea at Different Ripening Stages

et al. 2019

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Gray mold caused by Botrytis cinerea is a major cause of economic losses in strawberry fruit production, limiting fruit shelf life and commercialization. When the fungus infects Fragaria × ananassa strawberry at flowering or unripe fruit stages, symptoms develop after an extended latent phase on ripe fruits before or after harvesting. To elucidate the growth kinetics of B. cinerea on flower/fruit and the molecular responses associated with low susceptibility of unripe fruit stages, woodland strawberry Fragaria vesca flowers and fruits, at unripe white and ripe red stages, were inoculated with B. cinerea. Quantification of fungal genomic DNA within 72 h postinoculation (hpi) showed limited fungal growth on open flower and white fruit, while on red fruit, the growth was exponential starting from 24 hpi and sporulation was observed within 48 hpi. RNA sequencing applied to white and red fruit at 24 hpi showed that a total of 2,141 genes (12.5% of the total expressed genes) were differentially expressed due to B. cinerea infection. A broad transcriptional reprogramming was observed in both unripe and ripe fruits, involving in particular receptor and signaling, secondary metabolites, and defense response pathways. Membrane-localized receptor-like kinases and nucleotide-binding site leucine-rich repeat genes were predominant in the surveillance system of the fruits, most of them being downregulated in white fruits and upregulated in red fruits. In general, unripe fruits exhibited a stronger defense response than red fruits. Genes encoding for pathogenesis-related proteins and flavonoid polyphenols as well as genes involved in cell-wall strengthening were upregulated, while cell-softening genes appeared to be switched off. As a result, B. cinerea remained quiescent in white fruits, while it was able to colonize ripe red fruits.

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Section: Resultsmentioning

confidence: 89%

Transcriptome Profiles of Strawberry (Fragaria vesca) Fruit Interacting With Botrytis cinerea at Different Ripening Stages

et al. 2019

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“…Besides compactness, different berry skin features seem to influence the susceptibility of grapevines towards B. cinerea infection, i.e. , the biochemical composition [ 10 , 11 , 12 , 13 ], the ripening stage [ 10 , 12 ] and the morphology of the berry skin [ 11 , 14 ]. Especially, the cuticle and its epicuticular waxes are described as important berry skin features regarding the susceptibility of berries toward bunch rot [ 11 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Impedance of the Grape Berry Cuticle as a Novel Phenotypic Trait to Estimate Resistance to Botrytis Cinerea

Herzog

Wind

Töpfer

2015

Sensors

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Warm and moist weather conditions during berry ripening provoke Botrytis cinerea (B. cinerea) causing notable bunch rot on susceptible grapevines with the effect of reduced yield and wine quality. Resistance donors of genetic loci to increase B. cinerea resistance are widely unknown. Promising traits of resistance are represented by physical features like the thickness and permeability of the grape berry cuticle. Sensor-based phenotyping methods or genetic markers are rare for such traits. In the present study, the simple-to-handle I-sensor was developed. The sensor enables the fast and reliable measurement of electrical impedance of the grape berry cuticles and its epicuticular waxes (CW). Statistical experiments revealed highly significant correlations between relative impedance of CW and the resistance of grapevines to B. cinerea. Thus, the relative impedance Zrel of CW was identified as the most important phenotypic factor with regard to the prediction of grapevine resistance to B. cinerea. An ordinal logistic regression analysis revealed a R2McFadden of 0.37 and confirmed the application of Zrel of CW for the prediction of bunch infection and in this way as novel phenotyping trait. Applying the I-sensor, a preliminary QTL region was identified indicating that the novel phenotypic trait is as well a valuable tool for genetic analyses.

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“…Gene expression (fold change) was calculated using Hellemans method (Hellemans et al, 2007). The reference genes used for the normalization were Elongation factor 1-alpha (EF1α) (Pessina, 2016) and Glyceraldehyde-3phosphate dehydrogenase (GADPH) (Welter et al, 2017) for the V. vinifera-E. necator interaction and EF1α (Gruau et al, 2015) and SAND family protein (SAND) (Nanni et al, 2014)…”

Section: Sequências Oligonucleotídicas Dos Genes De Interesse E De Referência Usadas No Presente Estudomentioning

confidence: 99%

Pathogen-related specificity of subtilase VVISBT4.19 X1 in the Vitis vinifera defence response

Figueiredo

Cunha

Eiras-Dias

et al. 2020

Ciência Téc. Vitiv.

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Grapevine, Vitis vinifera L., is one of the most cultivated fruit plants worldwide with high economic value. Powdery mildew and gray mold diseases, caused by Erysiphe necator and Botrytis cinerea, respectively, are within the most devastating diseases, which are controlled by using several fungicide applications over a single growing season. A more sustainable and environmentally friendly alternative for pest control is associated to the development of breeding programs, in which American and Asian Vitis species, presenting natural resistance characteristics, are crossed with V. vinifera varieties that are susceptible to diseases caused by fungal or oomycete pathogens. As a result, new grapevine varieties that combine the good berry quality with a high degree of resistance to grapevine pathogens are obtained. One example is the Vitis vinifera cv ‘Regent’ that acquired high tolerance degree against E. necator and Plasmopara viticola. To ensure durable resistance introgression in breeding programs, a full understanding of grapevine defence mechanisms is crucial. Previous studies on grapevine-P. viticola pathosystem have suggested the participation of serine proteases in the establishment of the interaction between both organisms, which is the case of VviSBT4.19 X1. The gene expression of this subtilase increases up to 300-fold 6 hours after ‘Regent’ inoculation with P. viticola. Nowadays, no information is available about the participation of subtilases in grapevine response to E. necator and B. cinerea infection. In the present study, the gene expression profile of VviSBT4.19 X1 in the first hours of ‘Regent’ inoculation with E. necator and B. cinerea was analysed to understand its response towards different pathogenic agents.

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VvAMP2, a grapevine flower‐specific defensin capable of inhibiting Botrytis cinerea growth: insights into its mode of action

Cited by 18 publications

References 37 publications

Transcriptome Profiles of Strawberry (Fragaria vesca) Fruit Interacting With Botrytis cinerea at Different Ripening Stages

Transcriptome Profiles of Strawberry (Fragaria vesca) Fruit Interacting With Botrytis cinerea at Different Ripening Stages

Impedance of the Grape Berry Cuticle as a Novel Phenotypic Trait to Estimate Resistance to Botrytis Cinerea

Pathogen-related specificity of subtilase VVISBT4.19 X1 in the Vitis vinifera defence response

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