Wafer scale fabrication of graphene microelectrode arrays for the detection of DNA hybridization

Campos, Rui; Machado, George; Cerqueira, M. F.; Borme, Jérôme; Alpuim, P.

doi:10.1016/j.mee.2017.12.015

Cited by 21 publications

(17 citation statements)

References 26 publications

(35 reference statements)

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“…Finally, DNA has been shown to bind directly to graphene without the need of a linker, making it highly interesting for biosensor development ( Campos et al, 2018 ). Field-effect transistors (FET) with two-dimensional channels made of monolayer graphene have been developed to achieve label-free detection of DNA hybridization down to attomolar concentration, while offering at the same time the possibility of discriminating single nucleotide polymorphism ( Campos et al, 2019 ).…”

Section: Coating Applicationsmentioning

confidence: 99%

Interactions Between 2D Materials and Living Matter: A Review on Graphene and Hexagonal Boron Nitride Coatings

Santos

Moschetta

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

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Two-dimensional material (2DM) coatings exhibit complex and controversial interactions with biological matter, having shown in different contexts to induce bacterial cell death and contribute to mammalian cell growth and proliferation in vitro and tissue differentiation in vivo. Although several reports indicate that the morphologic and electronic properties of the coating, as well as its surface features (e.g., crystallinity, wettability, and chemistry), play a key role in the biological interaction, these kinds of interactions have not been fully understood yet. In this review, we report and classify the cellular interaction mechanisms observed in graphene and hexagonal boron nitride (hBN) coatings. Graphene and hBN were chosen as study materials to gauge the effect of two atomic-thick coatings with analogous lattice structure yet dissimilar electrical properties upon contact with living matter, allowing to discern among the observed effects and link them to specific material properties. In our analysis, we also considered the influence of crystallinity and surface roughness, detailing the mechanisms of interaction that make specific coatings of these 2DMs either hostile toward bacterial cells or innocuous for mammalian cells. In doing this, we discriminate among the material and surface properties, which are often strictly connected to the 2DM production technique, coating deposition and post-processing method. Building on this knowledge, the selection of 2DM coatings based on their specific characteristics will allow to engineer desired functionalities and devices. Antibacterial coatings to prevent biofouling, biocompatible platforms suitable for biomedical applications (e.g., wound healing, tissue repairing and regeneration, and novel biosensing devices) could be realized in the next future. Overall, a clear understanding on how the 2DM coating’s properties may modulate a specific bacterial or cellular response is crucial for any future innovation in the field.

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Section: Coating Applicationsmentioning

confidence: 99%

Interactions Between 2D Materials and Living Matter: A Review on Graphene and Hexagonal Boron Nitride Coatings

Santos

Moschetta

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

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show abstract

“…From that vantage point, limitations associated with foundry processes would not constrain sensor development and the more expensive CMOS chips could be reused. Adding on to that, work on combining less commonly employed sensor materials and electrochemical measurements with semiconductor industry processes is still being undertaken (Blair et al, 2018;Campos et al, 2018;Casteleijn et al, 2018;Kindlundh et al, 2003). With all of this in mind, there are several possible paths to the mass production of cheap, point-of-care systems for a whole range of diseases and one of the major aims of future work will be realization of microfabricated lab-on-chip DNA biosensors.…”

Section: Future Perspectivesmentioning

confidence: 99%

A review of microfabricated electrochemical biosensors for DNA detection

Blair

Corrigan

2019

Biosensors and Bioelectronics

130

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This review article presents an overview of recent work on electrochemical biosensors developed using microfabrication processes, particularly sensors used to achieve sensitive and specific detection of DNA sequences. Such devices are important as they lend themselves to miniaturisation, reproducible mass-manufacture, and integration with other previously existing technologies and production methods. The review describes the current state of these biosensors, novel methods used to produce them or enhance their sensing properties, and pathways to deployment of a complete point-ofcare biosensing system in a clinical setting.

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“…Regarding DNA probe immobilization at the surface of the WE by simple adsorption, hexagonal cells of graphene sheets and the aromatic rings of nucleobases share Van der Waals forces, ionic interactions, π–π stacking, and hydrogen bonds. DNA is physically adsorbed better on reduced GO than GO, since rGO has more aromatic regions for π–π stacking interactions with DNA bases and a lower surface charge density lessens electrostatic repulsion of the negatively charge DNA backbone [87, 107, 126].…”

Section: Aspects Affecting Stability And/or Reproducibility Of Electrmentioning

confidence: 99%

Impact of nano-morphology, lattice defects and conductivity on the performance of graphene based electrochemical biosensors

Tite¹,

Chiticaru²,

Burns³

et al. 2019

J Nanobiotechnol

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Diverse properties of graphenic materials have been extensively explored to determine properties that make good electrochemical nanomaterial-based biosensors. These are reviewed by critically examining the influence of graphene nano-morphology, lattice defects and conductivity. Stability, reproducibility and fabrication are discussed together with sensitivity and selectivity. We provide an outlook on future directions for building efficient electrochemical biosensors.

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Wafer scale fabrication of graphene microelectrode arrays for the detection of DNA hybridization

Cited by 21 publications

References 26 publications

Interactions Between 2D Materials and Living Matter: A Review on Graphene and Hexagonal Boron Nitride Coatings

Interactions Between 2D Materials and Living Matter: A Review on Graphene and Hexagonal Boron Nitride Coatings

A review of microfabricated electrochemical biosensors for DNA detection

Impact of nano-morphology, lattice defects and conductivity on the performance of graphene based electrochemical biosensors

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