Water adsorption with relative humidity changes for keratin and collagen as studied by infrared (<scp>IR</scp>) micro‐spectroscopy

Kudo, S.; Nakashima, Satoru

doi:10.1111/srt.12641

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…In this study, the CH band areas (the band at 2900 cm −1 ) of the treetop and lower-crown leaves decreased with RH (Figure 6A) and were negatively correlated with the band area of free water (Figure 6B). These trends were similar to previous reports of interactions between water molecules and biomolecules, such as collagen and lecithin [26,34,35]. Because the structures of the main components of plant tissues, such as cellulose, hemicellulose, and pectin, have CH, CH 2 , and CH 3 groups adjacent to polar functional groups, these correlations imply that free water with longer hydrogen bonds could be loosely bound to hydrophobic CH surfaces of polysaccharides in the leaf cross-sections of C. japonica.…”

Section: Discussionsupporting

confidence: 90%

Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control

Azuma

Nakashima

Yamakita

et al. 2020

Plants

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Leaf water storage is a complex interaction between live tissue properties (anatomy and physiology) and physicochemical properties of biomolecules and water. How leaves adsorb water molecules based on interactions between biomolecules and water, including hydrogen bonding, challenges our understanding of hydraulic acclimation in tall trees where leaves are exposed to more water stress. Here, we used infrared (IR) microspectroscopy with changing relative humidity (RH) on leaves of tall Cryptomeria japonica trees. OH band areas correlating with water content were larger for treetop (52 m) than for lower-crown (19 m) leaves, regardless of relative humidity (RH). This high water adsorption in treetop leaves was not explained by polysaccharides such as Ca-bridged pectin, but could be attributed to the greater cross-sectional area of the transfusion tissue. In both treetop and lower-crown leaves, the band areas of long (free water: around 3550 cm−1) and short (bound water: around 3200 cm−1) hydrogen bonding OH components showed similar increases with increasing RH, while the band area of free water was larger at the treetop leaves regardless of RH. Free water molecules with longer H bonds were considered to be adsorbed loosely to hydrophobic CH surfaces of polysaccharides in the leaf-cross sections.

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Section: Discussionsupporting

confidence: 90%

Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control

Azuma

Nakashima

Yamakita

et al. 2020

Plants

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“…In fact, decrease of band intensities (band areas) of CH and CO bands with increasing relative humidity have been reported during water adsorption to biomolecules. 30,31…”

Section: Discussionmentioning

confidence: 99%

Interactions of Glycerol, Diglycerol, and Water Studied Using Attenuated Total Reflection Infrared Spectroscopy

2020

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In order to examine the mixing properties of glycerol–water and diglycerol–water solutions, these solutions were measured using attenuated total reflection infrared spectroscopy. The absorbance spectra corrected for 1 µm thickness were subtracted by pure polyols for obtaining water spectra, and by pure water for polyol spectra. Both asymmetric and symmetric CH2 stretching vibration bands (around 2940, 2885 cm−1) shifted about 10 cm−1 to lower wavenumber side (redshifts) with increasing polyol concentrations, especially at higher concentrations. Redshifts of C–O–H rocking bands (around 1335 cm−1) with increasing polyol concentrations are slightly larger for diglycerol–water (10 > 6 cm−1) than glycerol–water solutions. C–O stretching bands of CHOH groups (1125 and 1112 cm−1) shift slightly but in opposite sides for glycerol and diglycerol at highest polyol concentrations (90–100 wt%). These shifts of CH2 stretching, COH rocking, and CO stretching of CHOH at higher polyol concentrations suggest interactions of outer CH2 with inner CHOH groups of surrounding polyols. The normalized band area changes with polyol concentrations could be fitted by quadratic polynomials possibly due to mixtures of different interactions between water–water, polyol–water, and polyol–polyol molecules. The OH stretching band for diglycerol 90 wt% shows three humps indicating at least three OH components: long, medium, and short H bond water molecules. Short H bond water molecules are the major component possibly between inner CHOH and outer side CH2OH groups, while the long H component might loosely bind to outer CH2OH groups.

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“…The stratum corneum is the only structure in which the moisture content changes depending on the external humidity 3 . The structure of the corneocyte changes according to the moisture change in the stratum corneum 4 . If it contains moisture, the volume may expand up to four times.…”

Section: Introductionmentioning

confidence: 99%

“…If it contains moisture, the volume may expand up to four times. In addition, the structure of keratin also changes between the alpha‐helix structure and the beta‐sheet according to the change of the moisture content 4,5 . When water is insufficient, the ratio of alpha‐helix increases, and stiffness also increases 6 …”

Section: Introductionmentioning

confidence: 99%

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Arginine–fructose–glucose from red ginseng extract reduces stiffness of keratin fiber in corneocyte of skin

Kim

Lee

2022

Skin Research and Technology

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Purpose:The moisture content of the stratum corneum of the skin changes depending on the external environment. The structure of keratinous fiber protein in corneocyte of the skin changes depending on the amount of moisture. As the moisture decreases, the population of the alpha-helix increases, the beta-sheet deceases, and the stiffness increases accordingly. Here, we investigated the effect of humectants from ginseng on the keratin structure.Methods: Corneocyte was prepared from dry porcine skin with disc tape and measured through ATR-FT-IR. The signal from amide I of the keratin protein in corneocyte was detected, and the change in the ratio of alpha-helix and beta-sheet was calculated. The test samples were treated on the exfoliated corneocyte, and the degree of change was checked.Result: Arginine-fructose-glucose (AFG)-enriched extract of red ginseng was effective in changing the keratin structure and was superior to humectants such as glycerin.However, arginine, mono sugar were not effective, and the AFG form in which two sugars were bound to one amino acid could perform its function. Conclusion:The present study suggests that AFG, when applied to cosmetics, is expected to improve skin texture in a different way from existing moisturizers represented by glycerin by reducing the alpha-helix structure of corneocyte keratin.

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Water adsorption with relative humidity changes for keratin and collagen as studied by infrared (IR) micro‐spectroscopy

Cited by 8 publications

References 28 publications

Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control

Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control

Interactions of Glycerol, Diglycerol, and Water Studied Using Attenuated Total Reflection Infrared Spectroscopy

Arginine–fructose–glucose from red ginseng extract reduces stiffness of keratin fiber in corneocyte of skin

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