Water-insoluble enzymes. Synthesis of a new carrier and its utilization for preparation of insoluble derivatives of papain, trypsin, and subtilopeptidase A

Goldstein, Léon; Pecht, M; Blumberg, Shmaryahu; Atlas, Daphné; Levin, Yehuda

doi:10.1021/bi00813a016

Cited by 91 publications

(20 citation statements)

References 31 publications

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“…This effect can be due to the positively charged unsubstituted amidino groups on the polymer [12,13]. It should be noted that the pH-activity profile for bound urease is broader than that for the corresponding free enzyme [14], as shown in Fig.2.…”

Section: Resultsmentioning

confidence: 99%

Coupling of Enzymes to Polyacrylonitrile

et al. 1976

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Commercial polyacrylonitrile was converted to the imidoester derivative and its capacity, degree of substitution and storage stability were tested.Urease, glucose oxidase and lactate dehydrogenase were coupled to the activated polymer by an amidino linkage. Some properties of the immobilized enzymes were studied and compared with those of the free enzymes.The use of enzymes bound to water-insoluble matrixes is an important branch of modern biotechnology. Many techniques have been developed so far for the immobilization of proteins onto polymers [l -31 and much work has been devoted to the preparation of an ideal matrix.

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Section: Resultsmentioning

confidence: 99%

Coupling of Enzymes to Polyacrylonitrile

et al. 1976

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“…It was shown that the pH-activity prof'des of polyanionic derivatives of several proteolytic enzymes, such as the ethylene maleic acid copolymer derivative of trypsin, are displaced by 1 to 2.5 pH units towards the alkaline side as compared to the native enzyme [42]. Polycationic derivatives of the same enzyme exhibited the reverse behavior displacing the pH optimum to more acidic pH values [43].…”

Section: Microenvironmental Matrix-effectsmentioning

confidence: 99%

Immobilised enzymes

Mosbach¹

1976

FEBS Letters

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“…However, the adsorption is quite low and most of the ALP in the other experiments will be present in an immobilized form onto the support by a covalent linkage through the carbonate groups. 5.0 mL in 0.05M glycine buffer at pH 9; enzyme concentration 2.0 mg/mL; temperature 4OC.…”

Section: Immobilization Of Alkaline Phosphatase (Alp) Onto Pvca-jeff mentioning

confidence: 99%

Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) ‐jeffamine® hydrogel beads

Chen

Does

Bantjes

1993

J of Applied Polymer Sci

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SYNOPSISHydrogel-like polymers with reactive cyclic carbonate ( CCA) groups have been obtained by the cross-linking reaction of poly(viny1ene carbonate) (PVCA) with Jeffamines@. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.

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Water-insoluble enzymes. Synthesis of a new carrier and its utilization for preparation of insoluble derivatives of papain, trypsin, and subtilopeptidase A

Cited by 91 publications

References 31 publications

Coupling of Enzymes to Polyacrylonitrile

Coupling of Enzymes to Polyacrylonitrile

Immobilised enzymes

Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) ‐jeffamine® hydrogel beads

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