What a Fish Can Learn from a Mouse: Principles and Strategies for Modeling Human Cancer in Mice

Bailey, Jennifer M.; Creamer, Bradley A.; Hollingsworth, Michael A.

doi:10.1089/zeb.2009.0626

Cited by 20 publications

(16 citation statements)

References 103 publications

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“…However, this model is often considered time-consuming and labor intensive. Zebrafish ( Danio rerio ) and their transparent embryos recently emerge as another promising xenograft tumor model system in tumor therapeutic drug screening [15], [16]. Zebrafish provides unique features for investigating tumor development, angiogenesis, invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

A Novel Zebrafish Xenotransplantation Model for Study of Glioma Stem Cell Invasion

et al. 2013

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Invasion and metastasis of solid tumors are the major causes of death in cancer patients. Cancer stem cells (CSCs) constitute a small fraction of tumor cell population, but play a critical role in tumor invasion and metastasis. The xenograft of tumor cells in immunodeficient mice is one of commonly used in vivo models to study the invasion and metastasis of cancer cells. However, this model is time-consuming and labor intensive. Zebrafish (Danio rerio) and their transparent embryos are emerging as a promising xenograft tumor model system for studies of tumor invasion. In this study, we established a tumor invasion model by using zebrafish embryo xenografted with human glioblastoma cell line U87 and its derived cancer stem cells (CSCs). We found that CSCs-enriched from U87 cells spreaded via the vessels within zebrafish embryos and such cells displayed an extremely high level of invasiveness which was associated with the up-regulated MMP-9 by CSCs. The invasion of glioma CSCs (GSCs) in zebrafish embryos was markedly inhibited by an MMP-9 inhibitor. Thus, our zebrafish embryo model is considered a cost-effective approach tostudies of the mechanisms underlying the invasion of CSCs and suitable for high-throughput screening of novel anti-tumor invasion/metastasis agents.

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Section: Introductionmentioning

confidence: 99%

A Novel Zebrafish Xenotransplantation Model for Study of Glioma Stem Cell Invasion

et al. 2013

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“…Embryo development is extremely rapid during the first few days postfertilization while the embryos and larvae are transparent, giving the possibility to study the in vivo organ development and perturbations. 4 These features, combined with easy handling in experimental conditions, enable the assessment of ionizing radiation (IR) effects on zebrafish development 5 and for studying radiation response modifiers. The combination strategy, using radiation in conjunction with radiation modifiers, is designed to make the tumor cells more susceptible to radiation damage and/or to selectively protect the healthy tissues surrounding the tumor.…”

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confidence: 99%

l-Alpha Glycerylphosphorylcholine as a Potential Radioprotective Agent in Zebrafish Embryo Model

et al. 2016

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This work establishes the zebrafish embryo model for ionizing radiation (IR) modifier research and also evaluates the protective effect of L-alpha glycerylphosphorylcholine (GPC). Embryos were exposed to a single-fraction wholebody gamma irradiation (5, 10, 15, and 20 Gy) at different postfertilization time points and were serially assessed for viability and macro-and micromorphologic abnormalities. After toxicity evaluation, 194 lM of GPC was added for certain groups with 3-h incubation before the radiation. Nuclear factor kappa B (NF-jB) and interleukin-1b (IL-1b) expression changes were measured using quantitative real-time polymerase chain reaction. A higher sensitivity could be observed at earlier stages of the embryogenesis. The lethal dose (LD 50 ) for 6 hours postfertilization (hpf) embryos was 15 Gy and for 24 hpf was 20 Gy on day 7, respectively. GPC administration resulted in a significant improvement in both the distortion rate and survival of the 24 hpf embryos. Qualitative evaluation of the histological changes confirmed the protective effect of GPC. IL-1b and NF-jB overexpression due to 10 Gy irradiation was also reduced by GPC. GPC exhibited promising radioprotective effects in our zebrafish embryo model, decreasing the irradiationinduced morphological damage and lethality with significant reduction of IR-caused pro-inflammatory activation.

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“…1). The FLP/ FRT system has been widely utilized in many higher eukaryotes such as plants, Caenorhabditis, mice, somatic cell lines and in embryonic stem cells 1415161718. In Saccharomyces , the recombination efficiency of FLP allowed the generation of a large number of applications such as a marker recycling system for multiple gene disruption 19 and “sticking” a gene wherever in the yeast genome (STIK = s pecific t argeted i ntegration of k anamycin resistance DNA) without a resident selective marker 20.…”

Section: Enzyme-dependent Site-specific Recombination Technologies Tmentioning

confidence: 99%

Per aspera ad astra: When harmful chromosomal translocations become a plus value in genetic evolution. Lessons from Saccharomyces cerevisiae.

Tosato

Bruschi

2015

MIC

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In this review we will focus on chromosomal translocations (either spontaneous or induced) in budding yeast. Indeed, very few organisms tolerate so well aneuploidy like Saccharomyces, allowing in depth studies on chromosomal numerical aberrations. Many wild type strains naturally develop chromosomal rearrangements while adapting to different environmental conditions. Translocations, in particular, are valuable not only because they naturally drive species evolution, but because they might allow the artificial generation of new strains that can be optimized for industrial purposes. In this area, several methodologies to artificially trigger chromosomal translocations have been conceived in the past years, such as the chromosomal fragmentation vector (CFV) technique, the Cre-loxP procedure, the FLP/FRT recombination method and, recently, the bridge - induced translocation (BIT) system. An overview of the methodologies to generate chromosomal translocations in yeast will be presented and discussed considering advantages and drawbacks of each technology, focusing in particular on the recent BIT system. Translocants are important for clinical studies because translocated yeast cells resemble cancer cells from morphological and physiological points of view and because the translocation event ensues in a transcriptional de-regulation with a subsequent multi-factorial genetic adaptation to new, selective environmental conditions. The phenomenon of post-translocational adaptation (PTA) is discussed, providing some new unpublished data and proposing the hypothesis that translocations may drive evolution through adaptive genetic selection.

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What a Fish Can Learn from a Mouse: Principles and Strategies for Modeling Human Cancer in Mice

Cited by 20 publications

References 103 publications

A Novel Zebrafish Xenotransplantation Model for Study of Glioma Stem Cell Invasion

A Novel Zebrafish Xenotransplantation Model for Study of Glioma Stem Cell Invasion

l-Alpha Glycerylphosphorylcholine as a Potential Radioprotective Agent in Zebrafish Embryo Model

Per aspera ad astra: When harmful chromosomal translocations become a plus value in genetic evolution. Lessons from Saccharomyces cerevisiae.

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