Whey protein fractionation: Isoelectric precipitation of α-lactalbumin under gentle heat treatment

Bramaud, C.; Aimar, Pierre; Daufin, G.

doi:10.1002/(sici)1097-0290(19971120)56:4<391::aid-bit5>3.0.co;2-j

Cited by 72 publications

(29 citation statements)

References 16 publications

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“…Protein movement across the membrane is based merely on the size exclusion and isoelectric properties of the proteins. Table shows the isoelectric points and molecular weights of the individual whey proteins . However, the molecular weight of lysozyme and lactalbumin are lesser and closer to each other.…”

Section: Resultsmentioning

confidence: 98%

Concentration of whey protein from cheese whey effluent using ultrafiltration by combination of hydrophilic metal oxides and hydrophobic polymer

Yogarathinam

Arthanareeswaran

Ismail

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND In this study, hydrophilic metal oxides such as TiO2, ZrO2 and ZnO were used as modifier for the preparation of polyethersulfone (PES) mixed matrix membranes (MMMs) for the filtration of cheese whey effluent. Nano metal oxide loading concentration was varied from 0.5 to 2.5 wt%. RESULTS Characterization of metal oxide studies show that the particles are hydrophilic and nanoscaled. ZnO nanoparticles are in the form of nanocluster and of size 14.28–20.10 nm. Physio‐chemical characterization of PES MMMs indicated that the nano metal oxides dispersed well in the PES matrix. Contact angle decreased up to 59.88° for 1.5 wt% TiO2 embedded PES (TiO2ePES) MMMs. Also, 1.5 wt% TiO2ePES MMMs gives a higher water permeability of 4.62 × 10–7 m s–1 kPa–1. Among membranes, 1.5 wt% TiO2ePES, 2.0 wt% ZrO2ePES and 1.5 wt% ZnOePES MMMs showed better flux, lower percentage fouling and better rejection for individual whey proteins. CONCLUSION The whey protein from cheese whey effluent was concentrated up to maximum of 43.9 mg L–1 using 2.0 wt% ZrO2ePES MMMs. It was found that 1.5 wt% TiO2ePES MMMs gives a higher average flux of 25.09 L m–2 h–1 and a lower flux reduction percentage of 39%. © 2018 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 98%

Concentration of whey protein from cheese whey effluent using ultrafiltration by combination of hydrophilic metal oxides and hydrophobic polymer

Yogarathinam

Arthanareeswaran

Ismail

et al. 2018

J of Chemical Tech & Biotech

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“…One possible explanation for this observation is the majority of the molecules used in this study have isoelectric points nearer to pH 8 than pH 5; therefore neutralization to pH 8 may increase hydrophobic interactions between product species that could lead to soluble or insoluble aggregate formation (e.g., precipitation). Certain proteins are known to precipitate at pH values near their pI as electrostatic forces between molecules are minimized . This could lead to lower amounts of soluble monomeric product in the filtrate.…”

Section: Resultsmentioning

confidence: 99%

Improved HCP Reduction Using a New, All‐Synthetic Depth Filtration Media Within an Antibody Purification Process

Nguyen

Langland

Amara³

et al. 2018

Biotechnology Journal

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Biologic manufacturing processes typically employ clarification technologies like depth filtration to remove insoluble and soluble impurities. Conventional depth filtration media used in these processes contain naturally-derived components like diatomaceous earth and cellulose. These components may introduce performance variability and contribute extractable/leachable components like beta-glucans that could interfere with limulus amebocyte lysate endotoxin assays. Recently a novel, all-synthetic depth filtration media is developed (Millistak+ HC Pro X0SP) that may improve process consistency, efficiency, and drug substance product quality by reducing soluble process impurities. This new media is evaluated against commercially available benchmark filters containing naturally-derived components (Millistak+ HC X0HC and B1HC). Using model proteins, the synthetic media demonstrates increased binding capacity of positively charged proteins (72-126 mg g media) compared to conventional media (0.3-8.6 mg g media); and similar values for negatively charged species (1.3-5.6 mg g media). Several CHO-derived monoclonal antibodies (mAbs) or mAb-like molecules are also evaluated. The X0SP filtration performance behaves similarly to benchmarks, and exhibits improved HCP reduction (at least 50% in 55% of cases tested). X0SP filtrates contained increased silicon extractables relative to benchmarks, but these were readily removed downstream. Finally, the X0SP devices demonstrates suitable lot-to-lot robustness when specific media components are altered intentionally to manufacturing specification limits.

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“…21 Whey solids were separated by ultraltration followed by pH (3.9), citrate (40 Â 10 À3 mol l À1 ) and temperature (45 C) treatment which results in calcium-protein complexation and precipitation of major whey proteins. Also to determine the role of total whey proteins in enhancing the production of CPT by the fungus, major protein fraction was precipitated from whey and the fungus was allowed to ferment the de-proteinized whey under submerged conditions.…”

Section: Effect Of Whey and Whey Proteins On Cpt Productionmentioning

confidence: 99%

Solid state fermentation of soybean waste and an up-flow column bioreactor for continuous production of camptothecine by an endophytic fungus Fusarium oxysporum

et al. 2016

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The potential of an endophytic fungus Fusarium oxysporum (NCIM 1383) to produce the anti-cancer prodrug 'camptothecine (CPT)' by solid state fermentation (SSF) of agro-industrial by-products was investigated. Amongst the various substrates used under optimized SSF conditions, soybean meal was found to be the most efficient medium for optimum production of CPT by the fungus. The effect of the addition of different complex protein sources to the soybean meal substrate on productivity by the fungus was also studied. The CPT production was found to be increased by 46.87% after supplementation of "whey concentrate powder" as a complex protein source to the soybean meal. The optimized SSF conditions were used to develop a bench-scale, up flow bioreactor to check its applicability for continuous CPT production which could yield up to 128 mg l À1 CPT after 48 h of incubation. Whey, when used as a source of moisture in SSF, also was found to play a vital role in enhancing the CPT yield. Environmental parameters such as chemical oxygen demand (COD), biological oxygen demand (BOD), total dissolved solids (TDS), total soluble solids (TSS) and total protein content of whey effluent after fermentation were found to be reduced significantly highlighting the environmental applications of the process developed. The SSF methodology applied in the present study allows bioutilization of cheaper substrates; soybean meal and whey, by the endophytic fungus for production of valuable anti-cancer pro drug CPT.

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Whey protein fractionation: Isoelectric precipitation of α-lactalbumin under gentle heat treatment

Cited by 72 publications

References 16 publications

Concentration of whey protein from cheese whey effluent using ultrafiltration by combination of hydrophilic metal oxides and hydrophobic polymer

Concentration of whey protein from cheese whey effluent using ultrafiltration by combination of hydrophilic metal oxides and hydrophobic polymer

Improved HCP Reduction Using a New, All‐Synthetic Depth Filtration Media Within an Antibody Purification Process

Solid state fermentation of soybean waste and an up-flow column bioreactor for continuous production of camptothecine by an endophytic fungus Fusarium oxysporum

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