Whole-genome expression analysis of Rice black-streaked dwarf virus in different plant hosts and small brown planthopper

Xu, Qiufang; Ni, Haiping; Zhang, Jinfeng; Lan, Ying; Ren, Chunmei; Zhou, Yijun

doi:10.1016/j.gene.2015.07.008

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…Further investigation showed that P7-1 binds tightly to the exon-10-encoded peptide and C-terminal 67 residues of RabGDIα in maize. Of 13 viral genes, S7-1 stands out as the only gene highly expressed at the earliest time point of viral infection 43 . P7-1 forms virus-containing tubules at plasmodesmata, presumably to assist viral intercellular movement and symptom development 32,33 .…”

Section: Discussionmentioning

confidence: 99%

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A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

et al. 2020

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Maize rough dwarf disease (MRDD), caused by various species of the genus Fijivirus, threatens maize production worldwide. We previously identified a quantitative locus qMrdd1 conferring recessive resistance to one causal species, rice black-streaked dwarf virus (RBSDV). Here, we show that Rab GDP dissociation inhibitor alpha (RabGDIα) is the host susceptibility factor for RBSDV. The viral P7-1 protein binds tightly to the exon-10 and C-terminal regions of RabGDIα to recruit it for viral infection. Insertion of a helitron transposon into RabGDIα intron 10 creates alternative splicing to replace the wild-type exon 10 with a helitron-derived exon 10. The resultant splicing variant RabGDIα-hel has difficulty being recruited by P7-1, thus leading to quantitative recessive resistance to MRDD. All naturally occurring resistance alleles may have arisen from a recent single helitron insertion event. These resistance alleles are valuable to improve maize resistance to MRDD and potentially to engineer RBSDV resistance in other crops.

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Section: Discussionmentioning

confidence: 99%

“…6a). Thus, it is natural for RBSDV to produce abundant P7-1 to recruit RabGDIα for successful viral infection 43 . Coincidentally, RBSDV-induced damage of the host transport system results in the MRDD syndrome, such as severe plant stunting, shortened internodes, malformed tassels, ears, and among others.…”

Section: Discussionmentioning

confidence: 99%

A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

et al. 2020

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“…Rice black-streaked dwarf virus (RBSDV; genus Fijivirus , family Reoviridae ), transmitted by the small brown planthopper (SBPH; Laodelphax striatellus Fallén), causes serious maize and rice losses worldwide, particularly in China [1,2]. Previous studies demonstrated the unique RBSDV S5 among ten ds-RNA segments with two partially overlapping ORFs but in a different reading frame [3].…”

Section: Introductionmentioning

confidence: 99%

Molecular characteristics of segment 5, a unique fragment encoding two partially overlapping ORFs in the genome of rice black-streaked dwarf virus

Zhang

Yao

et al. 2019

PLoS ONE

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Rice black-streaked dwarf virus (RBSDV), a ds-RNA virus in Fijivirus genus with family Reoviridae, which is transmitted by the small brown planthopper, is responsible for incidence of maize rough dwarf disease (MRDD) and rice black-streaked dwarf disease (RBSDD). To understand the variation and evolution of S5, a unique fragment in the genome of RBSDV which encodes two partially overlapping ORFs (ORF5-1 and ORF5-2), we analyzed 127 sequences from maize and rice exhibiting symptoms of dwarfism. The nucleotide diversity of both ORF5-1 (π = 0.039) and ORF5-2 (π = 0.027) was higher than that of the overlapping region (π = 0.011) (P < 0.05). ORF5-2 was under the greatest selection pressure based on codon bias analysis, and its activation was possibly influenced by the overlapping region. The recombinant fragments of three recombinant events (14NM23, 14BM20, and 14NM17) cross the overlapping region. Based on neighbor-joining tree analysis, the overlapping region could represent the evolutionary basis of the full-length S5, which was classified into three main groups. RBSDV populations were expanding and haplotype diversity resulted mainly from the overlapping region. The genetic differentiation of combinations (T127-B35, T127-J34, A58-B35, A58-J34, and B35-J34) reached significant or extremely significant levels. Gene flow was most frequent between subpopulations A58 and B35, with the smallest |Fst| (0.02930). We investigated interactions between 13 RBSDV proteins by two-hybrid screening assays and identified interactions between P5-1/P6, P6/P9-1, and P3/P6. We also observed self-interactive effects of P3, P6, P7-1, and P10. In short, we have proven that RBSDV populations were expanding and the overlapping region plays an important role in the genetic variation and evolution of RBSDV S5. Our results enable ongoing research into the evolutionary history of RBSDV-S5 with two partly overlapping ORFs.

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“…Accurate phenotypic evaluation is critical for marker-trait association analyses 39 . As with all viral diseases, MRDD requires interactions among an effective vector, the virulent pathogen, a susceptible host, and an appropriate environment 40 . Wheat is one of the preferred hosts for the planthopper insect vector ( L .…”

Section: Discussionmentioning

confidence: 99%

Identification of a locus conferring dominant resistance to maize rough dwarf disease in maize

Song

Wang

et al. 2018

Sci Rep

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Maize rough dwarf disease (MRDD) is a severe viral disease of maize that occurs worldwide, particularly in the summer maize-growing areas in China, resulting in yield losses and quality deterioration in susceptible maize varieties. An effective solution to control MRDD is to use resistance genes to improve the behavior of susceptible genotypes. Here, we employed maize F2 populations derived from a cross between susceptible line S221 and resistant line K36 for the deep sequencing of the two DNA pools containing extremely resistant and susceptible F2 individuals, and used traditional linkage analysis to locate the resistance-related genomic region. The results showed that MRDD resistance in K36 was controlled by a single dominant locus, and an associated region was identified within the genomic interval of 68,396,487 bp and 69,523,478 bp on chromosome 6. Two simple sequence repeat (SSR) markers 6F29R29 and 6F34R34 were tightly linked to the MRDD resistance locus. The findings of the present study improve our understanding of the inheritance patterns of MRDD resistance, and should inform MRDD-resistant maize breeding programs.

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Whole-genome expression analysis of Rice black-streaked dwarf virus in different plant hosts and small brown planthopper

Cited by 10 publications

References 33 publications

A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

Molecular characteristics of segment 5, a unique fragment encoding two partially overlapping ORFs in the genome of rice black-streaked dwarf virus

Identification of a locus conferring dominant resistance to maize rough dwarf disease in maize

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