Whole‐Genome <scp>mRNA</scp> Expression Profiling Identifies Functional and Prognostic Signatures in Patients with Mesenchymal Glioblastoma Multiforme

Bao, Zhaoshi; Zhang, Chuanbao; Wang, Hongjun; Yan, Wei; Liu, Yanwei; Li, Mingyang; Zhang, Wei

doi:10.1111/cns.12118

Cited by 28 publications

(40 citation statements)

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“…Since LHX6 involves in the developmental process of nervous system, it may influence the development of gliomas. Bao et al () identified that LHX6 was significantly related to the overall survival of patients with mesenchymal glioblastoma multiforme, and predicted a better prognosis. However, the biological function of LHX6 and its interaction with miRNAs in gliomas have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

MiR‐1290 promotes proliferation, migration, and invasion of glioma cells by targeting LHX6

Liu

Cai

Sun

et al. 2018

Journal Cellular Physiology

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To investigate the interaction of miR-1290 and LHX6 in gliomas, and their influence on the propagation and metastasis of glioma cells. The differential expression of miR-1290 in glioma cells was identified by chip screening. The expression level of miR-1290 and LHX6 were determined by qRT-PCR and Western blot. The influence of miR-1290 on propagation of glioma cells were analyzed by MTT assay, EdU incorporation, and colony formation, while the impact of miR-1290 on metastasis was assessed by transwell assay. The relationship between LHX6 and miR-1290 was testified by luciferase reporter assay. The gliomas orthotopic implantation model of nude mice was established to investigate the influence of miR-1290 and LHX6 on tumor growth. Tumor volumes were evaluated by photon density, and the expression of Ki67 protein was analyzed by immunohistochemistry. MiR-1290 presented a higher expression in glioma cells and tissues. MiR-1290 overexpression significantly promoted propagation and metastasis of glioma cells, while miR-1290 knockdown inhibited glioma development. MiR-1290 suppressed LHX6 expression, facilitating development of glioma cells. The orthotopic implantation model showed that miR-1290 overexpression promoted tumor growth while LHX6 overexpression inhibited it. MiR-1290 could promote glioma cell propagation and metastasis by inhibiting LHX6.

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Section: Introductionmentioning

confidence: 99%

MiR‐1290 promotes proliferation, migration, and invasion of glioma cells by targeting LHX6

Liu

Cai

Sun

et al. 2018

Journal Cellular Physiology

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“…Gliomas are the most common primary tumors in the central nervous system. Malignant glioblastoma multiforme (GBM), World Health Organization (WHO) grade IV, has a median survival of only 12–15 months . Currently, resection of all enhancing tumor with adjuvant chemo‐ and radiotherapies is the standard of care but provides limited benefit.…”

Section: Introductionmentioning

confidence: 99%

AJAP1 is Dysregulated at an Early Stage of Gliomagenesis and Suppresses Invasion Through Cytoskeleton Reorganization

Han

Zhang

et al. 2014

CNS Neurosci Ther

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SUMMARY Aims Down-regulation of AJAP1 in glioblastoma multiforme (GBM) has been reported. However, the expression profiles of AJAP1 in gliomas and the underlying mechanisms of AJAP1 function on invasion are still poorly understood. Methods The gene profiles of AJAP1 in glioma patients were studied among four independent cohorts. Confocal imaging was used to analyze the AJAP1 localization. After AJAP1 overexpression in GBM cell lines, cellular polarity, cytoskeleton distribution, and antitumor effect were investigated in vitro and in vivo. Results AJAP1 expression was significantly decreased in gliomas compared with normal brain in REMBRANDT and CGCA cohorts. Additionally, low AJAP1 expression was associated with worse survival in GBMs in REMBRANDT and TCGA U133A cohorts and was significantly associated with classical and mesenchymal subtypes of GBMs among four cohorts. Confocal imaging indicated AJAP1 localized in cell membranes in low-grade gliomas and AJAP1-overexpressing GBM cells, but difficult to assess in high-grade gliomas due to its absence. AJAP1 overexpression altered the cytoskeleton and cellular polarity in vitro and inhibited the tumor growth in vivo. Conclusions AJAP1 is dysregulated at an early stage of gliomagenesis and may suppress glioma cell invasion and proliferation, which suggests that AJAP1 may be a potential diagnostic and prognostic marker for gliomas.

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“…Glioblastoma, the most common malignant primary brain tumor, carries an invariably poor prognosis (3, 5, 6). Targeting underlying biological foundations of the disease will be crucial to developing more effective treatment strategies (3, 5, 6).…”

Section: Introductionmentioning

confidence: 99%

“…Targeting underlying biological foundations of the disease will be crucial to developing more effective treatment strategies (3, 5, 6). Transcriptional profiling through microarray analysis and protein expression profiling through immunohistochemistry (IHC)-based microarrays represent vital resources for researchers seeking to accomplish these goals (3, 5, 6). …”

Section: Introductionmentioning

confidence: 99%

Microarray Analysis in Glioblastomas

Bhawe

Aghi

2015

Methods in Molecular Biology

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Microarray analysis in glioblastomas is done using either cell lines or patient samples as starting material. A survey of the current literature points to transcript-based microarrays and immunohistochemistry (IHC)-based tissue microarrays as being the preferred methods of choice in cancers of neurological origin. Microarray analysis may be carried out for various purposes including the following: To correlate gene expression signatures of glioblastoma cell lines or tumors with response to chemotherapy (DeLay et al., Clin Cancer Res 18(10):2930–2942, 2012)To correlate gene expression patterns with biological features like proliferation or invasiveness of the glioblastoma cells (Jiang et al., PLoS One 8(6):e66008, 2013)To discover new tumor classificatory systems based on gene expression signature, and to correlate therapeutic response and prognosis with these signatures (Huse et al., Annu Rev Med 64(1):59–70, 2013; Verhaak et al., Cancer Cell 17(1):98–110, 2010) While investigators can sometimes use archived tumor gene expression data available from repositories such as the NCBI Gene Expression Omnibus to answer their questions, new arrays must often be run to adequately answer specific questions. Here, we provide a detailed description of microarray methodologies, how to select the appropriate methodology for a given question, and analytical strategies that can be used. Experimental methodology for protein microarrays is outside the scope of this chapter, but basic sample preparation techniques for transcript-based microarrays are included here.

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Whole‐Genome mRNA Expression Profiling Identifies Functional and Prognostic Signatures in Patients with Mesenchymal Glioblastoma Multiforme

Abstract: The signatures were identified as risk predictors that patients who had a high-risk score tended to have unfavorable outcome, demonstrating their potential for personalizing cancer management.

Cited by 28 publications

References 28 publications

MiR‐1290 promotes proliferation, migration, and invasion of glioma cells by targeting LHX6

MiR‐1290 promotes proliferation, migration, and invasion of glioma cells by targeting LHX6

AJAP1 is Dysregulated at an Early Stage of Gliomagenesis and Suppresses Invasion Through Cytoskeleton Reorganization

Microarray Analysis in Glioblastomas

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